A5037588517- Neonatal Respiratory Health Research
- Renal and related cancers
- Immune cells in cancer
- COVID-19 Clinical Research Studies
- Chromosomal and Genetic Variations
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Congenital Diaphragmatic Hernia Studies
- CRISPR and Genetic Engineering
- Gene expression and cancer classification
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Immune responses and vaccinations
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Respiratory Support and Mechanisms
Guangzhou Institutes of Biomedicine and Health
2020-2024
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2020-2024
Chinese Academy of Sciences
2020-2023
University of Chinese Academy of Sciences
2020-2023
Abstract The lung is the primary respiratory organ in human, which proximal airway and distal alveoli are responsible for air conduction gas exchange, respectively. However, regulation of proximal–distal patterning at embryonic stage human development largely unknown. Here we investigated early embryos weeks 4–8 post fertilization (Carnegie stages 12–21) using single-cell RNA sequencing, obtained a transcriptomic atlas 169,686 cells. We observed discernible gene expression patterns epithelia...
Cell fate decision involves rewiring of the genome, but remains poorly understood at chromatin level. Here, we report that remodeling complex NuRD participates in closing open early phase somatic reprogramming. Sall4, Jdp2, Glis1 and Esrrb can reprogram MEFs to iPSCs efficiently, only Sall4 is indispensable capable recruiting endogenous components NuRD. Yet knocking down reduces reprogramming modestly, contrast disrupting known Sall4-NuRD interaction by mutating or deleting interacting motif...
Abstract Background Single-cell RNA sequencing is becoming a powerful tool to identify cell states, reconstruct developmental trajectories, and deconvolute spatial expression. The rapid development of computational methods promotes the insight heterogeneous single-cell data. An increasing number tools have been provided for biological analysts, which two programming languages- R Python are widely used among researchers. complementary, as many implemented specifically in or Python. However,...
The vastly spreading COVID-19 pneumonia is caused by SARS-CoV-2. Lymphopenia and cytokine levels are tightly associated with disease severity. However, virus-induced immune dysregulation at cellular molecular remains largely undefined. Here, the leukocytes in pleural effusion, sputum, peripheral blood biopsies from severe mild patients were analyzed single-cell resolution. Drastic T cell hyperactivation accompanying elevated exhaustion was observed, predominantly effusion. mechanistic...
Abstract Deficiency of the histone H3K9 methyltransferase SETDB1 induces RIPK3-dependent necroptosis in mouse embryonic stem cells (mESCs). However, how pathway is activated this process remains elusive. Here we report that reactivation transposable elements (TEs) upon knockout responsible for RIPK3 regulation through both cis and trans mechanisms. IAPLTR2_Mm MMERVK10c-int, which are suppressed by SETDB1-dependent H3K9me3, act as enhancer-like cis-regulatory their nearby members enhance...