A5057047486- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- RNA Research and Splicing
- DNA Repair Mechanisms
- Mitochondrial Function and Pathology
- Tissue Engineering and Regenerative Medicine
- Neonatal Respiratory Health Research
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- 3D Printing in Biomedical Research
- Congenital heart defects research
- RNA Interference and Gene Delivery
- Wound Healing and Treatments
- Mesenchymal stem cell research
- Cancer Cells and Metastasis
- Animal Genetics and Reproduction
- Developmental Biology and Gene Regulation
- Chromatin Remodeling and Cancer
- FOXO transcription factor regulation
- Graphene and Nanomaterials Applications
- Genetics and Neurodevelopmental Disorders
- Bone Tissue Engineering Materials
- Healthcare and Venom Research
Guangzhou Medical University
2017-2025
Guangzhou Experimental Station
2024
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2020-2023
Hainan Medical University
2023
Chinese Academy of Sciences
2015-2022
Guangzhou Institutes of Biomedicine and Health
2015-2021
University of Chinese Academy of Sciences
2017-2019
Abstract The lung is the primary respiratory organ in human, which proximal airway and distal alveoli are responsible for air conduction gas exchange, respectively. However, regulation of proximal–distal patterning at embryonic stage human development largely unknown. Here we investigated early embryos weeks 4–8 post fertilization (Carnegie stages 12–21) using single-cell RNA sequencing, obtained a transcriptomic atlas 169,686 cells. We observed discernible gene expression patterns epithelia...
Highlights•Jdp2, Jhdm1b, Mkk6, Glis1, Nanog, Esrrb, and Sall4 (7F) convert MEFs into iPSCs•RNA-seq ATAC-seq reveal a distinct path for 7F reprogramming•7F cooperate to open close chromatin during activate TF network induce pluripotencySummaryReprogramming somatic cells pluripotency by Oct4, Sox2, Klf4, Myc represent paradigm cell fate determination. Here, we report combination of Jdp2, Essrb, that reprogram mouse embryonic fibroblasts or chimera competent induced pluripotent stem (iPSCs)...
Abstract Peripheral blood mesenchymal stem cells (PBMSCs) may be easily harvested from patients, permitting autologous grafts for bone tissue engineering in the future. However, PBMSC’s capabilities of survival, osteogenesis and production new matrix defect area are still unclear. Herein, PBMSCs were seeded into a nanofiber scaffold self-assembling peptide (SAP) cultured osteogenic medium. The results indicated SAP can serve as promising survival differentiation 3D conditions. Furthermore,...
Polycomb repressive complex 1 (PRC1) plays essential roles in cell-fate determination. Recent studies have found that the composition of mammalian PRC1 is particularly varied and complex; however, little known about functional consequences these variant complexes on Here, we show Kdm2b promotes Oct4-induced somatic reprogramming through recruitment a (PRC1.1) to CpG islands (CGIs). Furthermore, find bone morphogenetic protein (BMP) represses Oct4/Kdm2b-induced selectively. Mechanistically,...
The transition from pluripotent to somatic states marks a critical event in mammalian development, but remains largely unresolved. Here we report the identification of SS18 as regulator for or PST by CRISPR-based whole genome screens. Mechanistically, forms microscopic condensates nuclei through C-terminal intrinsically disordered region (IDR) rich tyrosine, which, once mutated, no longer form nor rescue SS18-/- defect PST. Yet, IDR alone is not sufficient even though it can...
Abstract Forkhead box (Fox) transcription factors play important roles in mammalian development and disease. However, their function mouse somatic cell reprogramming remains unclear. Here, we report that FoxD subfamily FoxG1 accelerate induced pluripotent stem cells (iPSCs) generation from fibroblasts as early day4 while FoxA FoxO impede this process obviously. More importantly, FoxD3, FoxD4 can replace Oct4 respectively generate iPSCs with germline transmission together Sox2 Klf4. On the...
Abstract Multiple pluripotent states have been described in mouse and human stem cells. Here, we apply single-cell RNA-seq to a newly established BMP4 induced primed naïve transition (BiPNT) system show that the reset is not direct reversal of cell fate but goes through primordial germ cell-like cells (PGCLCs) state. We first epiblast bifurcate into c-Kit + − trophoblast-like cells, among which, branch undergoes further PGCLCs intermediate capable spermatogenesis vivo. Mechanistically, DOT1L...
Transposable elements (TEs) are the major sources of lineage-specific genomic innovation and comprise nearly half human genome, but most their functions remain unclear. Here, we identify that a series endogenous retroviruses (ERVs), TE subclass, regulate transcriptome at definitive endoderm stage with in vitro differentiation model from embryonic stem cell. Notably, these ERVs perform as enhancers containing binding sites for critical transcription factors lineage specification. Genome-wide...
Generation of intestinal organoids from human somatic cells by reprogramming would enable regeneration, disease modeling, and drug screening in a personalized pattern. Here, we report direct protocol for the generation urine induced (U-iIOs) under defined medium. U-iIOs expressed multiple specific genes showed resembling gene expression profiles to primary small intestines. can be stably long-term expanded further differentiated into more mature lineage with high metallothionein cytochrome...
Numerous studies have shown that somite development is a necessary stage of myogenesis chondrogenesis and osteogenesis. Our previous study has established stable presomitic mesoderm progenitor cell line (UiPSM) in vitro. Naturally, we wanted to explore whether UiPSM can develop bone myogenic differentiation.
Abstract Background The kidneys require vast amounts of mitochondria to provide ample energy reabsorb nutrients and regulate electrolyte, fluid, blood pressure homeostasis. lack the human model hinders investigation homeostasis related kidney physiology disease. Results Here, we report generation mitochondria-rich organoids via partial reprogramming urine cells (hUCs) under defined medium. First, reprogrammed hUCs into expandable intermediate mesoderm progenitor like (U-iIMPLCs), which in...
Reprogramming somatic cells to pluripotency represents a paradigm for cell fate determination. A binary logic of closing and opening chromatin provides simple way understand iPSC reprogramming driven by both Yamanaka factors or chemicals. Here we apply this design combination Jdp2, Jhdm1b, Mkk6, Glis1, Nanog, Essrb Sall4 (7F) that reprogram MEFs chimera competent iPSCs efficiently. RNA- ATAC-seq reveal distinct mechanisms 7F induction pluripotency. Dropout experiments further highly...