A5051729484- Genomics and Chromatin Dynamics
- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- RNA Research and Splicing
- RNA modifications and cancer
- Cancer-related gene regulation
- Renal and related cancers
- Chromosomal and Genetic Variations
- Plant Pathogens and Fungal Diseases
- Protein Degradation and Inhibitors
- Plant-Microbe Interactions and Immunity
- Ubiquitin and proteasome pathways
- Chromatin Remodeling and Cancer
- Cancer-related molecular mechanisms research
- Fungal Plant Pathogen Control
- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
- Congenital heart defects research
- Neuroblastoma Research and Treatments
- Genomic variations and chromosomal abnormalities
- Metabolism, Diabetes, and Cancer
- Genetics and Neurodevelopmental Disorders
- PARP inhibition in cancer therapy
- Plant nutrient uptake and metabolism
Guangzhou Institutes of Biomedicine and Health
2015-2025
Guangzhou Medical University
2017-2025
First Affiliated Hospital of Guangzhou Medical University
2023-2025
State Key Laboratory of Respiratory Disease
2020-2025
Chinese Academy of Sciences
2014-2024
University of Chinese Academy of Sciences
2004-2024
Center for Excellence in Molecular Cell Science
2017-2023
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2019-2022
University of Hong Kong
2021-2022
Pennsylvania State University
2008-2012
CCCTC-binding factor (CTCF) is a DNA-binding protein that plays important roles in chromatin organization, although the mechanism by which CTCF carries out these functions not fully understood. Recent studies show recruits cohesin complex to insulator sites and required for activity. Here we showed DEAD-box RNA helicase p68 (DDX5) its associated noncoding RNA, steroid receptor activator (SRA), form with essential function. was detected at IGF2/H19 imprinted control region (ICR) as well other...
Brd4 is a bromodomain protein that binds to acetylated chromatin. It regulates cell growth, although the underlying mechanism has remained elusive. also been shown control transcription of viral genes, whereas its role in cellular genes not fully elucidated. Here we addressed growth and using small hairpin (sh) RNA approach. The shRNA vector stably knocked down expression by ∼90% NIH3T3 cells mouse embryonic fibroblasts. knockdown were impaired grew more slowly than cells. When synchronized...
To investigate effects of application 200 micromol l(-1) methyl jasmonate [MeJA (200)] and Cryptococcus laurentii alone or in combination against postharvest diseases (Monilinia fructicola Penicillium expansum) peach fruit stored at 25 0 degrees C, to evaluate the possible mechanisms involved.The efficacy controlling by resistance induced treated with MeJA (200) C. relationship between activities defence-related enzymes lesions caused M. P. expansum were examined. At same time, on population...
Histone Arg methylation has been correlated with transcriptional activation of p53 target genes. However, whether this modification is reversed to repress the expression genes unclear. Here, we report that peptidylarginine deiminase 4, a histone citrullination enzyme, involved in repression Inhibition or depletion PAD4 elevated subset genes, including p21/CIP1/WAF1, leading cell cycle arrest and apoptosis. Moreover, induction p21, arrest, apoptosis by dependent. Protein-protein interaction...
Protein Arg methyltransferases function as coactivators of the tumor suppressor p53 to regulate gene expression. Peptidylarginine deiminase 4 (PAD4/PADI4) counteracts functions protein in regulation by deimination and demethylimination. Here we show that expression a gene, OKL38, is activated inhibition PAD4 or activation following DNA damage. Chromatin immunoprecipitation assays showed dynamic change occupancy histone modifications at OKL38 promoter during damage, suggesting direct role...
Polycomb repressive complex 2 and the epigenetic mark that it deposits, H3K27me3, are evolutionarily conserved play critical roles in development cancer. However, their cell fate decisions early embryonic remain poorly understood. Here we report knockout of polycomb genes human stem cells causes pluripotency loss spontaneous differentiation toward a meso-endoderm fate, owing to de-repression BMP signalling. Moreover, with deletion EZH1 or EZH2 fail differentiate into ectoderm lineages. We...
Abstract Polycomb repressive complex 1 (PRC1) is an important regulator of gene expression and development. PRC1 contains the E3 ligases RING1A/B, which monoubiquitinate lysine 119 at histone H2A (H2AK119ub1), has been sub-classified into six major complexes based on presence a PCGF subunit. Here, we report that PCGF5, one paralogs, requirement in differentiation mouse embryonic stem cells (mESCs) towards neural cell fate. Although PCGF5 not required for mESC self-renewal, its loss blocks by...
R-loops modulate genome stability and regulate gene expression, but the functions regulatory mechanisms of in stem cell biology are still unclear. Here, we profiled during somatic reprogramming found that dynamic changes essential for occurred before expression. Disrupting homeostasis by depleting RNaseH1 or catalytic inactivation at D209 (RNaseH1D209N) blocks reprogramming. Sox2, not any other factor Yamanaka cocktail, overcomes inhibitory effects activity loss on Sox2 interacts with...
CTCF mediates chromatin insulation and long-distance enhancer-promoter (EP) interactions; however, little is known about how these regulatory functions are partitioned among target genes in key biological processes. Here, we show that Ctcf expression progressively increased during induced pluripotency. In this process, first as a insulator responsible for direct silencing of the somatic gene program and, interestingly, elevated next ensures accessibility contributes to EP interactions...
Super-enhancers (SEs) are cis-regulatory elements enriching lineage specific key transcription factors (TFs) to form hotspots. A paucity of identification and functional dissection promoted us investigate SEs during myoblast differentiation. ChIP-seq analysis histone marks leads the uncovering which remodel progressively course Further analyses TF enable definition SE hotspots co-bound by master TF, MyoD other TFs, among we perform in-depth for MyoD/FoxO3 interaction in driving formation...
CTCF plays key roles in gene regulation, chromatin insulation, imprinting, X chromosome inactivation and organizing the higher-order architecture of mammalian genomes. Previous studies have mainly focused on canonical isoform. Here, we explore functions an alternatively spliced human isoform which exons 3 4 are skipped, producing a shorter (CTCF-s). Functionally, find that CTCF-s competes with genome binding binds similar DNA sequence. disrupts CTCF/cohesin binding, alters CTCF-mediated...
CTCF plays a pivotal role in mediating chromatin interactions, but it does not do so alone. A number of factors have been reported to co-localize with and regulate loops, no comprehensive analysis binding partners has performed. This prompted us identify loop participants regulators by co-localization CTCF. We screened all that had ChIP-seq data humans human super conserved (hscCTCF) sites, identified many new overlapped hscCTCF sites. Combined information, we observed clustered could...
Abstract Although extended pluripotent stem cells (EPSCs) have the potential to form both embryonic and extraembryonic lineages, how their transcriptional regulatory mechanism differs from that of (ESCs) remains unclear. Here, we discovered YY1 binds specific open chromatin regions in EPSCs. Yy1 depletion EPSCs leads a gene expression pattern more similar ESCs than control Moreover, triggers series epigenetic crosstalk activities, including changes DNA methylation, histone modifications...
CCCTC-binding factor (CTCF) is a ubiquitously expressed "master weaver" and plays multiple functions in the genome, including transcriptional activation/repression, chromatin insulation, imprinting, X chromosome inactivation, high-order organization. It has been shown that CTCF facilitates recruitment of upstream binding onto ribosomal DNA (rDNA) regulates local epigenetic state rDNA repeats. However, mechanism by which modulates rRNA gene transcription not well understood. Here we found...
Ankyrin Repeat Domain-containing Protein 11 ( ANKRD11 ) is a causative gene for KBG syndrome, significant risk factor Cornelia de Lange syndrome (CdLS), and highly confident autism spectrum disorder gene. Mutations of lead to developmental abnormalities in multiple organs/tissues including the brain, craniofacial skeletal bones, tooth structures with unknown mechanism(s). Here, we find that ANKRD11, via short peptide fragment its N-terminal region, binds cohesin complex high affinity,...
R-loops are prevalent triplex nucleic strands found across various organisms, involved in numerous biological processes. However, the physiological and pathological functions of remain largely unknown due to a lack effective high-resolution detection methods. Here, using nuclease P1, T5 exonuclease, lambda exonuclease mediated digestion ssRNA, ssDNA, dsDNA while preserving RNA:DNA hybrid, we report method named R-loop identification assisted by high-throughput sequencing (RIAN-seq) for...