A5100444793- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Biosensors and Analytical Detection
- MicroRNA in disease regulation
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- RNA modifications and cancer
- Extracellular vesicles in disease
- Gold and Silver Nanoparticles Synthesis and Applications
- CRISPR and Genetic Engineering
- Cancer-related gene regulation
- Protein Degradation and Inhibitors
- Natural product bioactivities and synthesis
- Cancer Research and Treatments
- Genomics and Chromatin Dynamics
- Renal and related cancers
- Biochemical and Molecular Research
- Bacteriophages and microbial interactions
- Dendrimers and Hyperbranched Polymers
- DNA and Nucleic Acid Chemistry
- Galectins and Cancer Biology
- Histone Deacetylase Inhibitors Research
- Plant biochemistry and biosynthesis
- Mitochondrial Function and Pathology
- Oral microbiology and periodontitis research
Shenyang Pharmaceutical University
2024
Shanghai Jiao Tong University
2024
Hunan University
2018-2024
Qingdao University of Science and Technology
2023-2024
Nankai University
2018-2024
State Key Laboratory of Medicinal Chemical Biology
2024
Chinese PLA General Hospital
2024
Huazhong Agricultural University
2024
Shanghai Xuhui Central Hospital
2024
Fudan University
2024
Abstract Accurate detection of trace tetracyclines (TCs) in complex matrices is great significance for food and environmental safety monitoring. However, traditional recognition amplification tools exhibit poor specificity sensitivity. Herein, a novel dual‐machine linkage nanodevice (DMLD) proposed the first time to achieve high‐performance analysis TC, with padlock aptamer component as initiation command center, nucleic acid‐encoded multispike virus‐like Au nanoparticles (nMVANs) signal...
Abstract Molecular profiling of protein markers on small extracellular vesicles (sEVs) is a promising strategy for the precise detection and classification ovarian cancers. However, this challenging owing to lack simple practical methods. In work, using an aptamer‐based nanoflow cytometry (nFCM) strategy, rapid method molecular multiple sEVs was developed. The can be easily labeled with aptamer probes then rapidly profiled by nFCM. Seven cancer‐associated markers, including CA125, STIP1,...
Abnormal activation of fibroblast growth factor receptors (FGFRs) results in the development and progression human cancers. FGFR2 is frequently amplified or mutated cancers; therefore, it an attractive target for tumor therapy. Despite several pan-FGFR inhibitors, their long-term therapeutic efficacy hindered by acquired mutations low isoform selectivity. Herein, we report discovery efficient selective proteolysis-targeting chimeric molecule, LC-MB12, that incorporates essential rigid...
There has been growing interest in utilizing highly photostable iridium(III) complexes as new luminescent probes for biotechnology and life science. Herein, complex with carboxyl group was synthesized activated N-hydroxysuccinimide, followed by tagging to the amino terminate of single-stranded DNA (ssDNA). The Ir-ssDNA probe further combined graphene oxide (GO) nanosheets develop a GO-based biosensor target ssDNA detection. quenching efficiency GO, photostability GO-Ir-ssDNA biosensor, were...
Alzheimer's disease (AD) is a neurodegenerative disorder recognized as global public health priority. Although available treatments temporarily relieve the symptoms, they could not prevent progression of cognitive decline. Natural compounds have been rich sources for drug discovery. Dendrobium nobile Lindl. alkaloid (DNLA) main active compound in Lindl, traditional Chinese herbal medicine. Recent studies indicated that DNLA produced neuroprotection. However, mechanisms underlying...
As information messengers for cell-to-cell communication, exosomes, typically small membrane vesicles (30-150 nm), play an imperative role in the physiological and pathological processes of living systems. Accumulating studies have demonstrated that exosomes are potential biological candidates theranostics, including liquid biopsy-based diagnosis drug delivery. However, their clinical applications hindered by several issues, especially unspecific detection insufficient targeting ability. How...
An accelerated DNAzyme-based fluorescent nanoprobe was developed for rapid and highly sensitive detection of microRNA in live cells.
The interplay between the Yamanaka factors (OCT4, SOX2, KLF4 and c-MYC) transcriptional/epigenetic co-regulators in somatic cell reprogramming is incompletely understood. Here, we demonstrate that histone H3 lysine 27 trimethylation (H3K27me3) demethylase JMJD3 plays conflicting roles mouse reprogramming. On one side, induces pro-senescence factor Ink4a degrades pluripotency regulator PHF20 a factor-independent manner. other specifically recruited by to reduce H3K27me3 at both enhancers...
Current action mechanisms for aptamer-based therapeutics depend on occupancy-driven pharmacology to mediate protein functions. We report a new mechanism where aptamers leverage cellular proteasomal degradation system degrade proteins cancer treatment. A DNA aptamer (hereinafter referred as c-Met-Ap) binds the extracellular domain of mesenchymal-epithelial transition factor (c-Met) and selectively induces c-Met phosphorylation at Y1003 Y1349. The recruits E3 ubiquitin ligase casitas B-lineage...
Liver fibrosis is characterised by the activation of hepatic stellate cells (HSCs) and matrix deposition. Accumulating evidence has revealed that oncogenic protein tyrosine phosphatase Src homology 2 domain-containing (SHP2) acts as a therapeutic target fibrosis. Although several SHP2 inhibitors have reached early clinical trials, there are currently no FDA-approved drugs SHP2. In this study, we aimed to identify novel from an in-house natural product library treat liver Out screened 800...
The methodological development of nucleic acids detection is a rapidly growing research field. Here, we report powerful method to detect by an integration surface-enhanced Raman scattering and exonuclease III-assisted probe amplification. With unique signal-on strategy, have demonstrated that the target DNA MnSOD gene in concentrations as low 1 aM can reproducibly be detected, which offers limit several orders magnitude better than previous reports literature. new biosensor exhibits...