A5101528616- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Cancer, Hypoxia, and Metabolism
- interferon and immune responses
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- RNA regulation and disease
- Renal and related cancers
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Atherosclerosis and Cardiovascular Diseases
- Cancer Immunotherapy and Biomarkers
- Metabolomics and Mass Spectrometry Studies
- RNA Research and Splicing
- Liver Disease Diagnosis and Treatment
- Natural product bioactivities and synthesis
- Liver Disease and Transplantation
- Cytokine Signaling Pathways and Interactions
- Immune cells in cancer
- Lipid metabolism and disorders
- Genomics and Phylogenetic Studies
- Traditional Chinese Medicine Studies
- Genomics and Rare Diseases
Shenyang Agricultural University
2025
Sun Yat-sen University
2017-2024
Chinese PLA General Hospital
2024
Worcester Polytechnic Institute
2023-2024
China Medical University
2024
Tianjin Medical University
2024
Zhejiang University
2023
First Affiliated Hospital Zhejiang University
2023
University Health Network
2021-2022
Toronto General Hospital
2021-2022
Viruses evade the innate immune response by suppressing production or activity of cytokines such as type I interferons (IFNs). Here we report discovery a mechanism which SARS-CoV-2 virus coopts an intrinsic cellular machinery to suppress key immunostimulatory cytokine IFN-β. We reveal that encoded nonstructural protein 2 (NSP2) directly interacts with GIGYF2 protein. This interaction enhances binding mRNA cap-binding 4EHP, thereby repressing translation Ifnb1 mRNA. Depletion 4EHP...
Abstract Human ribosomes have long been thought to be uniform factories with little regulatory function. Accumulating evidence emphasizes the heterogeneity of ribosomal protein (RP) expression in specific cellular functions and development. However, a systematic understanding functional relevance RPs is lacking. Here, we surveyed translational transcriptional changes after individual knockdown 75 RPs, 44 from large subunit (60S) 31 small (40S), by Ribo-seq RNA-seq analyses. Deficiency...
Abstract A complete characterization of genetic variation is a fundamental goal human genome research. Long-read sequencing has improved the sensitivity structural variant discovery. Here, we conduct long-read sequencing-based analysis for 405 unrelated Chinese individuals, with 68 phenotypic and clinical measurements. We discover landscape 132,312 nonredundant variants, which 45.2% are novel. The identified variants high-quality, an estimated false discovery rate 3.2%. concatenated length...
Retinoblastoma (RB) is the most common primary intraocular malignancy of childhood. It known that tumor microenvironment (TME) regulates tumorigenesis and metastasis. However, how malignant progression in RB determined by heterogeneity cells TME remains uncharacterized. Here, we conducted integrative single-cell transcriptome whole-exome sequencing analysis patients with detailed pathological clinical measurements. By transcriptomic sequencing, profiled around 70,000 from samples seven...
Generation of induced pluripotent stem cells from somatic using defined factors has potential relevant applications in regenerative medicine and biology. However, this promising technology remains inefficient time consuming. We have devised a serum free culture medium termed iSF1 that facilitates the generation mouse cells. This optimization is sensitive to presence Myc reprogramming factors. Moreover, we could reprogram meningeal only two Oct4/Klf4. Therefore, represents basal may be used...
The current understanding of how overall principles translational control govern the embryo-to-adult transition in mammals is still far from comprehensive. Herein we profiled translatomes and transcriptomes six tissues mice at embryonic adult stages presented first report tissue- stage-specific landscape mice. We quantified extent gene expression divergence among different layers, stages, detected significant changes composition function underlying these divergences revealed changing...
The gut bacterial community plays many important roles in the production of nutrients and digestion. Antheraea pernyi A. yamamai (Lepidoptera: Saturniidae) are two traditional sources human food, as well being silk-producing insects. In present study, influences rearing season (spring autumn), silkworm species (A. yamamai), host plant (Quercus wutaishanica Salix viminalis) on microbiota diversity were tested using Illumina MiSeq technology. We found that composition larvae reared autumn...
Increasing evidence has highlighted the critical functions of immunogenic cell death (ICD) within many tumors. However, therapeutic possibilities and mechanism utilizing ICD in melanoma are still not well investigated. Melanoma samples involved our study were acquired from The Cancer Genome Atlas (TCGA) Gene Expression Omnibus (GEO) databases. First, pan-cancer analysis systematically revealed its expression characteristics, prognostic values, mutation information, methylation level, pathway...
Serum creatinine (Scr) may be not suited to timely and accurately reflect kidney injury related chronic liver disease. Currently, the ability of arterial spin labeling (ASL) blood oxygen level-dependent (BOLD) sequences evaluate renal flow (RBF) in disease remains verified.
Background Despite the comparatively low prevalence of osteosarcoma (OS) compared to other cancer types, metastatic OS has a poor overall survival rate fewer than 30%. Accumulating data shown crucial functions immunogenic cell death (ICD) in various cancers; nevertheless, relationship between ICD and was not previously well understood. This research aims determine function construct an ICD-based prognostic panel. Methods Single RNA sequencing from GSE162454 dataset distinguished malignant...
Retinal pigment epithelium (RPE) cells transfer oxygen and nutrients from choroid to the neural retina. Reduced RPE perturbs development functions of blood vessels in Previous efforts genome-wide studies have been largely focused on transcriptional changes response hypoxia. Recently developed ribosome profiling provides an opportunity study translational changes. To gain systemic insights into regulation cellular hypoxic stress, we used simultaneous RNA sequencing line, ARPE-19, under...
The significance of A-to-I RNA editing in nervous system development is widely recognized; however, its influence on retina remains to be thoroughly understood.
Abstract Atherosclerosis is a chronic inflammatory condition in which macrophages play major role. Janus kinase 2 (JAK2) pivotal molecule and metabolic signaling, Jak2 V617F activating mutation has recently been implicated with enhancing clonal hematopoiesis atherosclerosis. To determine the essential vivo role of macrophage (M)-Jak2 atherosclerosis, we generate atherosclerosis-prone ApoE-null mice deficient M-Jak2. Contrary to our expectation, these exhibit increased plaque burden no...
Autophagy is a physiological self-eating process that can promote cell survival or activate death in eukaryotic cells. In skeletal muscle, it important for maintaining muscle mass and function critical to sustain mobility regulate metabolism. The UV radiation resistance-associated gene (UVRAG) regulates the early stages of autophagy autophagosome maturation plays key role endosomal trafficking. This study investigated essential vivo UVRAG biology. To determine vivo, we generated...