A5047125151- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Epigenetics and DNA Methylation
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Circular RNAs in diseases
- Osteoarthritis Treatment and Mechanisms
- Zebrafish Biomedical Research Applications
- RNA Interference and Gene Delivery
- 3D Printing in Biomedical Research
- Pancreatic function and diabetes
- Biomedical Ethics and Regulation
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Neurogenesis and neuroplasticity mechanisms
- Acute Myeloid Leukemia Research
- Cancer-related gene regulation
- Developmental Biology and Gene Regulation
- Ocular Surface and Contact Lens
- Metabolism, Diabetes, and Cancer
- Protein Degradation and Inhibitors
- Diabetes Treatment and Management
- Advancements in Transdermal Drug Delivery
- Genomics and Chromatin Dynamics
Guangzhou Medical University
2017-2025
Guangzhou Institutes of Biomedicine and Health
2013-2024
Chinese Academy of Sciences
2012-2024
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2011-2023
University of Chinese Academy of Sciences
2023
Third Affiliated Hospital of Guangzhou Medical University
2022
University of Hong Kong
2017
Hefei National Center for Physical Sciences at Nanoscale
2011
University of Science and Technology of China
2011
MicroRNAs (miRNAs) are emerging critical regulators of cell function that frequently reside in clusters throughout the genome. They influence a myriad functions, including generation induced pluripotent stem cells, also termed reprogramming. Here, we have successfully delivered entire miRNA into reprogramming fibroblasts using retroviral vectors. This strategy avoids caveats associated with transient transfection chemically synthesized mimics. Overexpression 2 clusters, 106a-363 and...
Induced pluripotent stem cell (iPS cell) holds great potential for applications in regenerative medicine, drug discovery, and disease modeling. We describe here a practical method to generate human iPS cells from urine-derived (UCs) under feeder-free, virus-free, serum-free condition without oncogene c-MYC. showed that this approach could be applied large population with different genetic backgrounds. UCs are easily accessible exhibit high reprogramming efficiency, offering advantages over...
Abstract Reprogramming has been shown to involve EMT–MET; however, its role in cell differentiation is unclear. We report here that vitro of hESCs hepatic lineage undergoes a sequential EMT–MET with an obligatory intermediate mesenchymal phase. Gene expression analysis reveals Activin A-induced formation definitive endoderm (DE) accompanies synchronous EMT mediated by autocrine TGFβ signalling followed MET process. Pharmacological inhibition blocks the as well DE formation. then identify...
Polycomb repressive complex 2 and the epigenetic mark that it deposits, H3K27me3, are evolutionarily conserved play critical roles in development cancer. However, their cell fate decisions early embryonic remain poorly understood. Here we report knockout of polycomb genes human stem cells causes pluripotency loss spontaneous differentiation toward a meso-endoderm fate, owing to de-repression BMP signalling. Moreover, with deletion EZH1 or EZH2 fail differentiate into ectoderm lineages. We...
Neurogenesis, a highly orchestrated process, entails the transition from pluripotent to neural state and involves progenitor cells (NPCs) neuronal/glial subtypes. However, precise epigenetic mechanisms underlying fate decision remain poorly understood. Here, we delete KDM6s (JMJD3 and/or UTX), H3K27me3 demethylases, in human embryonic stem (hESCs) show that their deletion does not impede NPC generation hESCs. KDM6-deficient NPCs exhibit poor proliferation failure differentiate into neurons...
Osteoarthritis (OA) is one of the most common joint diseases and a major global public health concern. Mesenchymal stem cells (MSCs) have been widely used for treatment OA owing to their paracrine secretion trophic factors, phenomenon in which exosomes may play role. Here, we investigate potential from human umbilical cord-derived MSCs (hUC-MSCs-Exos) alleviating OA.The hUC-MSCs-Exos were harvested hUC-MSC-conditioned medium using ultracentrifugation. Rats with surgically-induced...
Human pluripotent stem cells (hPSCs) exhibit very limited contribution to interspecies chimeras. One explanation is that the conventional hPSCs are in a primed state and so unable form chimeras pre-implantation embryos. Here, we show undergo rapid apoptosis when injected into mouse While, forced-expression of BMI1, polycomb factor overcomes enables integrate embryos subsequently contribute with both embryonic extra-embryonic tissues. In addition, BMI1 also other species, such as rabbit pig....
Hematopoietic stem cells (HSCs)/progenitor (HPCs) are generated from hemogenic endothelial (HECs) during the endothelial-to-hematopoietic transition (EHT); however, underlying mechanism remains poorly understood. Here, using an array of approaches, including CRSPR/Cas9 gene knockouts, RNA-Seq, ChIP-Seq, ATAC-Seq etc., we report that vitamin C (Vc) is essential in HPC generation human pluripotent cell (hPSC) differentiation defined culture conditions. Mechanistically, found absence Vc fail to...
Inflammation of chondrocytes plays a critical role in the occurrence and development osteoarthritis (OA). Recent evidence indicated exosomes derived from mesenchymal stem cells (MSCs-Exos) exhibit excellent anti-inflammatory ability many troublesome inflammatory diseases including OA. In present study, we aimed to explore human umbilical cord-derived MSCs-Exos (hUC-MSCs-Exos) treating inflammation its related mechanisms. Ultracentrifugation was applied isolate hUC-MSCs-Exos culture...
As a minimally invasive drug delivery platform, microneedles (MNs) overcome many drawbacks of the conventional transdermal systems, therefore are favorable in biomedical applications. Microneedles with combined burst and sustained release profile maintained therapeutic molecular bioactivity could further broaden its applications as therapeutics. Here, we developed double-network (DN MNs) based on gelatin methacrylate acellular neural matrix (GelMA-ACNM). ACNM function an early matrix,...
During neurogenesis, neural patterning is a critical step during which progenitor cells differentiate into neurons with distinct functions. However, the molecular determinants that regulate remain poorly understood. Here we optimized "dual SMAD inhibition" method to specifically promote differentiation of human pluripotent stem (hPSCs) forebrain and hindbrain along rostral–caudal axis. We report determination occurs at very early stage in this differentiation. Undifferentiated hPSCs...
The transition from hemogenic endothelial cells (HECs) to hematopoietic stem/progenitor (HS/PCs), or (EHT), is a critical step during hematopoiesis. However, little known about the molecular determinants of HECs due challenge in defining HECs. We report here generation GATA2w/eGFP reporter human embryonic stem (hESCs) mark expressing GATA2, gene for EHT. show that differentiation, functional are almost exclusively GATA2/eGFP+. then constructed regulatory network HEC determination and also...
Dissociation-induced apoptosis is a striking phenomenon in human embryonic stem cells (hESCs), but not naive mouse ESCs. Rho-associated kinase–dependent actin-myosin hyperactivation an underlying mechanism that triggers dissociated hESCs; however, this study, we show the Ink4A-ARF–mediated senescence pathway another to cause individualized hESCs. We P16INK4A and P14ARF are immediately induced hESCs upon dissociation, Overexpression of BMI1, suppressor for Ink4A-ARF, greatly promotes survival...
The widely used lipid-lowering drug niacin was reported to increase blood glucose in diabetes. How does regulate β Cell function diabetic patients remains unclear. This study aimed investigate the effect of on cell lipotoxicity vitro and vivo. Niacin treatment sensitized palmitate-induced cytotoxicity apoptosis INS-1 cells. In addition, palmitate significantly increased receptor GPR109A PPARγ2 levels, which could be further boosted by co-treatment, creating a vicious cycle. contrast,...
More and more evidence showed that circRNA/miRNA/mRNA axis played a vital role in the pathogenesis of some diseases. However, partial bladder outlet obstruction (pBOO) remains unknown. Our study aimed to explore complex regulatory mechanism pBOO.The pBOO rat model was established, tissues were collected for mRNA sequencing. The differentially expressed mRNAs analyzed by high-throughput sequencing, GO KEGG analysis performed. Competing endogenous RNAs (ceRNAs) identified potential regulation...
Somatic cells can be reprogrammed into pluripotent stem (iPSCs) by overexpressing defined transcription factors. Specifically, overexpression of OCT4 alone has been demonstrated to reprogram mouse fibroblasts iPSCs. However, it remains unclear whether any other single factor induce iPSCs formation. Here, we report that SALL4 alone, under an optimized reprogramming medium iCD4, is capable Mechanistically, facilitates inhibiting somatic genes and activating genes, such as Esrrb Tfap2c....
ZBTB7A, a transcription factor containing tandem array of four Cys2-His2 zinc fingers (ZFs), is vital for multiple physiological events through directional binding to different genomic loci. Our previously determined crystal structure ZBTB7A in complex with GCCCCTTCCCC sequence revealed that all ZFs (ZF1-4) are involved γ-globin -200 gene element repress fetal haemoglobin expression. Recently, it has been reported drives primed-to-naïve transition (PNT) pluripotent stem cells 12-bp consensus...