A5027493675- Liver physiology and pathology
- Pluripotent Stem Cells Research
- Pancreatic function and diabetes
- CRISPR and Genetic Engineering
- Liver Disease Diagnosis and Treatment
- Hedgehog Signaling Pathway Studies
- Epigenetics and DNA Methylation
- Congenital heart defects research
- COVID-19 Clinical Research Studies
- Vitamin C and Antioxidants Research
- Cancer-related molecular mechanisms research
- Liver Disease and Transplantation
- 3D Printing in Biomedical Research
- Congenital Heart Disease Studies
- RNA Research and Splicing
- MicroRNA in disease regulation
- Organ Transplantation Techniques and Outcomes
- Renal and related cancers
- Drug-Induced Hepatotoxicity and Protection
- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Pediatric Hepatobiliary Diseases and Treatments
- Phagocytosis and Immune Regulation
- Pharmacological Receptor Mechanisms and Effects
- Genetics and Neurodevelopmental Disorders
Guangzhou Institutes of Biomedicine and Health
2014-2024
University of Chinese Academy of Sciences
2017-2024
State Key Laboratory of Respiratory Disease
2020-2024
Chinese Academy of Sciences
2014-2024
Guangzhou Medical University
2017-2024
Third Affiliated Hospital of Guangzhou Medical University
2024
Jinan University
2024
University of Science and Technology of China
2024
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2019-2022
Key Laboratory of Guangdong Province
2020-2021
Abstract Reprogramming has been shown to involve EMT–MET; however, its role in cell differentiation is unclear. We report here that vitro of hESCs hepatic lineage undergoes a sequential EMT–MET with an obligatory intermediate mesenchymal phase. Gene expression analysis reveals Activin A-induced formation definitive endoderm (DE) accompanies synchronous EMT mediated by autocrine TGFβ signalling followed MET process. Pharmacological inhibition blocks the as well DE formation. then identify...
Indian Hedgehog (Ihh) regulates tissue morphogenesis. (Hh) activity has been demonstrated in human cholangiocarcinoma and hepatocellular carcinoma lines, myofibroblasts progenitors from adult rodent livers. We evaluated Hh pathway involvement the response to biliary injury primary cirrhosis (PBC). Liver sections 3 PBC patients controls without liver disease were studied. Immunohistochemistry was used determine if cells that accumulate livers express Ihh or Hh-target genes including...
Both myofibroblastic hepatic stellate cells (HSC) and epithelial progenitors accumulate in damaged livers. In some injured organs, the ability to distinguish between fibroblastic is sometimes difficult because undergo epithelial-mesenchymal transitions (EMT). During EMT, coexpress mesenchymal cell markers. To determine whether EMT occurs adult liver cells, we analyzed expression profile of primary HSC, two HSC lines, progenitors. As expected, all expressed Surprisingly, these markers were...
Myocardial dysfunction is evident within hours after ablation of the cardiac neural crest in chick embryos, suggesting a role for myocardial maturation that separate from its outflow septation. This could be conserved an animal does not have divided systemic and pulmonary circulation, such as zebrafish. To test this hypothesis, we used cell marking to identify axial level migrates heart zebrafish embryos. Unlike mouse, originate somites. The region cranial somite 1 was found contribute cells...
β-arrestins are multifunctional proteins that act as scaffolds and transducers of intracellular signals from heptahelical transmembrane-spanning receptors (7TMR). Hedgehog (Hh) signaling, which uses the putative 7TMR, Smoothened, is established a fundamental pathway in development, unregulated Hh signaling associated with certain malignancies. Here, we show functional knockdown β-arrestin 2 zebrafish embryos recapitulates many phenotypes mutants. Expression wild-type 2, or constitutive...
The class III PI3-kinase (PIK3C3) is an enzyme responsible for the generation of phosphatidylinositol 3-phosphate (PI3P), a critical component vesicular membrane. Here, we report that PIK3C3 deficiency in zebrafish results intestinal injury and inflammation. In pik3c3 mutants, gut tube forms but fails to be maintained. Gene expression analysis reveals barrier-function-related inflammatory bowel disease (IBD) susceptibility genes (e-cadherin, hnf4a, ttc7a) are suppressed, while response...
Background: The pandemic of coronavirus disease 2019 (COVID-19) resulted in grave morbidity and mortality worldwide. There is currently no effective drug to cure COVID-19. Based on analyses available data, we deduced that excessive prostaglandin E 2 (PGE ) produced by cyclooxygenase-2 was a key pathological event Methods: A prospective clinical study conducted one hospital for COVID-19 treatment with Celebrex suppress the PGE production. total 44 cases were enrolled, 37 experimental group...
Liver fibrosis biomarker, Type IV collagen, may function as hepatocarcinogenesis niche. However, among the six isoforms, isoforms providing tumor microenvironment and their regulatory network are still unclarified. Based on bioinformatics analysis of hundreds HCC transcriptome datasets from public databases, we found that COL4A1/2 expressions were significantly correlated with hepatocarcinogenesis, progression, prognosis. The upregulated in preneoplastic tissues compared normal tissues....
Abstract Acute liver failure (ALF) involves extensive necrosis of cells and severe impairment function. Hepatocyte transplantation holds promise for treating ALF by swiftly supporting functions promoting regeneration. However, the scarcity suitable cell sources requires strategies to obtain enough functional hepatocyte‐like (HLCs) optimize their in vivo efficiency. A DNA hexahedral nanostructure (DHN) is developed loaded with microRNA‐122 efficiently induce hepatic differentiation human...
Abstract Liver fibrosis is a chronic pathological condition lacking specific clinical treatments. Stem cells, with notable potential in regenerative medicine, offer promise treating liver fibrosis. However, stem cell therapy hindered by immunological rejection, carcinogenesis risk, efficacy variation, and high cost. secretome‐based cell‐free offers solutions to address these challenges, but it limited low delivery efficiency rapid clearance. Herein, an innovative approach for situ...
A population genetic study identified that the asialoglycoprotein receptor 1 (ASGR1) mutation carriers had substantially lower non–HDL-cholesterol (non–HDL-c) levels and reduced risks of cardiovascular diseases. However, mechanism behind this phenomenon remained unclear. Here, we established Asgr1-knockout mice represented a plasma lipid profile with significantly non–HDL-c triglyceride (TG) caused by decreased secretion increased uptake VLDL/LDL. These 2 phenotypes were linked expression...
Background: Insulin resistance (IR) is the key pathological basis of many metabolic disorders. Lack asialoglycoprotein receptor 1 (ASGR1) decreased serum lipid levels and reduced risk coronary artery disease. However, whether ASGR1 also participates in regulatory network insulin sensitivity glucose metabolism remains unknown.Methods: The constructed knockout mice ASGR1-/- HepG2 cell lines were used to establish animal model syndrome IR by high-fat diet (HFD) or drug induction, respectively....
Abstract Transdifferentiation of other cell type into human neuronal cells (hNCs) provides a platform for neural disease modeling, drug screening and potential cell-based therapies. Among all the donor sources, urine (hUCs) are convenient to obtain without invasive harvest procedure. Here, we report novel approach transdifferentiation hUCs hNCs. Our study demonstrated that combination seven small molecules (CAYTFVB) cocktail induced These chemical-induced (CiNCs) exhibited typical...
Promoting liver regeneration while inhibiting fibrogenesis represented an attractive strategy for treating diseases, with hepatic stellate cells (HSCs) being crucial to both processes. This study aimed identify specific targets in HSCs that simultaneously facilitated and suppressed fibrosis, elucidated their molecular mechanisms. Through comparing acute chronic injury mouse models induced by CCl4 injections, we revealed exhibited dual functionality, expressing pro-regenerative pro-fibrogenic...
Heat stress-induced biochemical alterations in ovarian follicles compromise the function of granulosa cells (GCs) and developmental competence oocytes. Summer heat stress can have a far-reaching negative impact on overall fertility reproductive success. Together with stress, rise assisted technologies (ART), potential confounding hazards vitro handling absence systemic body support ART makes it imperative to study ameliorative effects vitamin C under conditions. Using treatment 43 °C for two...