A5016534711- Liver physiology and pathology
- Liver Disease Diagnosis and Treatment
- Pluripotent Stem Cells Research
- Metabolism, Diabetes, and Cancer
- Drug-Induced Hepatotoxicity and Protection
- Vitamin C and Antioxidants Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Organ Transplantation Techniques and Outcomes
- Single-cell and spatial transcriptomics
- Gene Regulatory Network Analysis
- COVID-19 Clinical Research Studies
- Liver Disease and Transplantation
- Pharmacological Receptor Mechanisms and Effects
- CRISPR and Genetic Engineering
- Metabolomics and Mass Spectrometry Studies
- Hedgehog Signaling Pathway Studies
- Lipid metabolism and biosynthesis
- Vitamin D Research Studies
- Cancer-related molecular mechanisms research
- Gene expression and cancer classification
- Lipid metabolism and disorders
- Medicinal Plant Pharmacodynamics Research
- Adipokines, Inflammation, and Metabolic Diseases
- Cancer, Lipids, and Metabolism
- Pancreatitis Pathology and Treatment
Chinese Academy of Sciences
2017-2024
Guangzhou Institutes of Biomedicine and Health
2017-2024
University of Chinese Academy of Sciences
2017-2022
Key Laboratory of Guangdong Province
2021
Background: The pandemic of coronavirus disease 2019 (COVID-19) resulted in grave morbidity and mortality worldwide. There is currently no effective drug to cure COVID-19. Based on analyses available data, we deduced that excessive prostaglandin E 2 (PGE ) produced by cyclooxygenase-2 was a key pathological event Methods: A prospective clinical study conducted one hospital for COVID-19 treatment with Celebrex suppress the PGE production. total 44 cases were enrolled, 37 experimental group...
Recently, single-cell RNA sequencing (scRNA-seq) and assay for transposase-accessible chromatin using (scATAC-seq) have been developed to separately measure transcriptomes accessibility profiles at the resolution. However, few methods can reliably integrate these data perform regulatory network analysis. Here, we integrated analysis (IReNA) inference through of scRNA-seq scATAC-seq data, modularization, transcription factor enrichment, construction simplified intermodular networks. Using...
Background: Insulin resistance (IR) is the key pathological basis of many metabolic disorders. Lack asialoglycoprotein receptor 1 (ASGR1) decreased serum lipid levels and reduced risk coronary artery disease. However, whether ASGR1 also participates in regulatory network insulin sensitivity glucose metabolism remains unknown.Methods: The constructed knockout mice ASGR1-/- HepG2 cell lines were used to establish animal model syndrome IR by high-fat diet (HFD) or drug induction, respectively....
A population genetic study identified that the asialoglycoprotein receptor 1 (ASGR1) mutation carriers had substantially lower non–HDL-cholesterol (non–HDL-c) levels and reduced risks of cardiovascular diseases. However, mechanism behind this phenomenon remained unclear. Here, we established Asgr1-knockout mice represented a plasma lipid profile with significantly non–HDL-c triglyceride (TG) caused by decreased secretion increased uptake VLDL/LDL. These 2 phenotypes were linked expression...
Abstract Secreted phospholipase A2s are involved in the development of obesity, type 2 diabetes mellitus (T2DM) and cardiovascular disease, which have become serious growing health concerns worldwide. Integration genome‐wide association study gene co‐expression networks analysis showed that secreted A2 group XIIA (PLA2G12A) may participate hepatic lipids metabolism. Nevertheless, role PLA2G12A lipid metabolism its potential mechanism remain elusive. Here, we used AAV9 vector carrying human...
Promoting liver regeneration while inhibiting fibrogenesis represented an attractive strategy for treating diseases, with hepatic stellate cells (HSCs) being crucial to both processes. This study aimed identify specific targets in HSCs that simultaneously facilitated and suppressed fibrosis, elucidated their molecular mechanisms. Through comparing acute chronic injury mouse models induced by CCl4 injections, we revealed exhibited dual functionality, expressing pro-regenerative pro-fibrogenic...
Abstract Background The world is under serious threat with the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes disease 2019 (COVID-19). However, there no effective drug for treatment COVID-19. Based on analyses available data, we deduced that excessive prostaglandins E (PGE ) accumulation mediated by cyclooxygenase-2 (COX-2) was key pathological basis Methods urine PGE levels were measured mass spectrometry. An experimental study about Celebrex to treat...
Abstract Background The limited proliferative ability of hepatocytes is a major limitation to meet their demand for cell-based therapy, bio-artificial liver device, and drug tests. One strategy amplify cells at the hepatoblast (HB) stage. However, expansion HBs with bipotency preserved challenging. Most HB methods hardly maintain also lack functional confirmation. Methods On basis analyzing manipulating related signaling pathways during (derived from human induced pluripotent stem cells,...
Chemically strategies to generate hepatic cells from human pluripotent stem (hPSCs) for the potential clinical application have been improved. However, producing high quality and large quantities of remain challenging, especially in terms step-wise efficacy cost-effective production requires more improvements. Here, we systematically evaluated chemical compounds hepatoblast (HB) expansion maturation establish a robust, cost-effective, reproducible methodology self-renewal HBs functional...
Hepatic stellate cells (HSCs) are crucial for liver injury repair and cirrhosis. However, the mechanism of chemotactic recruitment HSCs into loci is still largely unknown. Here, we demonstrate that serum amyloid A1 (SAA1) acts as a chemokine recruiting toward signaling via TLR2, finding proven by gene manipulation studies in cell mice models. The mechanistic investigations revealed SAA1/TLR2 axis stimulates Rac GTPases through PI3K-dependent pathways induces phosphorylation MLC (pSer19)....
Fatty liver is a reversible status, but also an origin stage to develop other metabolic syndromes, such as diabetes and heart disease that threatens public health worldwide. Ascorbate deficiency reported be correlated with increasing risks for whether ascorbate has therapeutic effect unknown. Here, we investigated if treatment alone could work on protecting from the development of steatosis mechanisms beyond.Guinea pigs were fed chow diet or high palm oil (HPD) respectively. HPD induced...
Abstract Background Acetaminophen (APAP) is the most commonly used non-prescription antipyretic and analgesic drugs. Overuse of APAP can cause hepatotoxicity. Liver sinusoidal endothelial cells (LSECs) damage an important early event in APAP-induced liver injury. Serum amyloid A (SAA) acute phase protein that mainly produced by hepatocytes, promotes dysfunction via a pro-inflammatory pro-thrombotic effect atherosclerosis renal disease. However, role SAA injury remains unclear. Methods In...
miR-122 is the most abundant miRNA in human liver, accounting for 52% of entire hepatic miRNome. Previous studies have demonstrated that plays key roles hepatocyte growth, metabolism, and homeostasis. Here, we created three knockout embryonic stem cell line lines, WAe001-A-7, WAe001-A-8, WAe001-A-9, using CRISPR/Cas9 technique. These mutated lines retained their pluripotency, vitro differentiation potential, normal morphology, karyotype.
MiR-122 is the most abundant miRNA in human liver accounting for 52% of entire hepatic miRNome. Previous studies have demonstrated that miR-122 a valuable therapeutic target diseases, including viral hepatitis, fibrosis, steatosis, and hepatocarcinoma. Here, we constructed doxycycline-inducible expression embryonic stem cell line WAe001-A-15 using piggyBac transposon system. The retained its pluripotency, vitro differentiation potential, normal morphology, karyotype.
Abstract Background: Chemically strategies to generate hepatic cells from human pluripotent stem (hPSCs) for the potential clinical application have been improved. However, producing high quality and large quantities of remain challenging, especially in terms step-wise efficacy cost-effective production requires more improvements. Methods: Here, we systematically evaluated chemical compounds hepatoblasts (HBs) expansion maturation establish a robust, reproducible methodology self-renewal HBs...
ABSTRACT As rapid progress in sequencing technology since last decade, numerous mechanisms underlying cell functions and developmental processes have been revealed as complex regulations of gene expressions. Since single-cell RNA (scRNA-seq) made high-resolution transcriptomic view increasingly accessible, precise identification regulatory network (GRN) describing types states became achievable. However, extracting key elements, including pathways (GRPs), transcription factors (TFs),...
Hepatic stellate cells (HSCs) are crucial for liver injury repair and cirrhosis. However, the mechanism of chemotactic recruitment HSCs into loci is still largely unknown. Here, by using CCl4- cryoinjury-induced acute models in C57/BL6 mice, we demonstrate that injury-induced hepatocytes secreted an phase protein, serum amyloid A (SAA) a chemokine attracting loci. The mRNA-based screening antagonist administration indicated toll-like receptor 2 (TLR2) was on responding to SAA, further...
Abstract Background Acute phase protein serum amyloid A (SAA) is one of abundantly expressed in the liver following injury or other traumas. However, relative contribution during inflammatory response remains largely elusive. Method SAA1 genetic knockdown was performed by injecting siRNA/invivofectamine3.0 complex CCl 4 -treated C57BL/6 mice. Parts excised were then processed for histological analyses. Primary human hepatocytes isolated from surgical specimens, and evaluated cell death,...