A5101663171- Pluripotent Stem Cells Research
- Immune Cell Function and Interaction
- CRISPR and Genetic Engineering
- CAR-T cell therapy research
- IL-33, ST2, and ILC Pathways
- Hematopoietic Stem Cell Transplantation
- Immune cells in cancer
- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- Zebrafish Biomedical Research Applications
- Acute Myeloid Leukemia Research
- Immunotherapy and Immune Responses
- Nanoparticles: synthesis and applications
- Cancer Cells and Metastasis
- Liver physiology and pathology
- RNA modifications and cancer
- Cancer Immunotherapy and Biomarkers
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Erythrocyte Function and Pathophysiology
- Liver Disease Diagnosis and Treatment
- Advanced Photocatalysis Techniques
- Adenosine and Purinergic Signaling
- TiO2 Photocatalysis and Solar Cells
- Renal and related cancers
- Healthcare and Environmental Waste Management
Guangzhou Institutes of Biomedicine and Health
2015-2025
Chinese Academy of Sciences
2016-2025
City Of Hope National Medical Center
2023-2025
University of Chinese Academy of Sciences
2015-2021
Guangzhou Regenerative Medicine and Health Guangdong Laboratory
2021
Guangzhou Medical University
2021
Quaid-i-Azam University
2017
Abstract Oct4, along with Sox2 and Klf4 (SK), can induce pluripotency but structurally similar factors like Oct6 cannot. To decode why Oct4 has this unique ability, we compare Oct4-binding, accessibility patterns transcriptional waves an mutant defective in the dimerization (Oct4 defSox2 ). We find that initial silencing of somatic program proceeds indistinguishably or without Oct4. mitigates mesenchymal-to-epithelial transition derails reprogramming. These effects are a consequence...
NKp46 is a critical regulator of natural killer (NK) cell immunity, but its function in non-NK innate immune cells remains unclear. Here, we show that indispensable for expressing IL-2 receptor-α (IL-2Rα) by liver-resident type-1 lymphoid (ILC1s). Deletion reduces IL-2Rα on ILC1s downregulating NF-κB signaling, thus impairing ILC1 proliferation and cytotoxicity vitro vivo. The binding anti-NKp46 antibody to triggers the activation NF-κB, expression IL-2Rα, interferon-γ (IFN-γ), tumor...
The RUNX1/AML1 transcription factor is one of the key regulators definitive hematopoietic development in mice. However, its role early human hematopoiesis remains poorly investigated. In this study, we integrated a tdTomato reporter cassette into RUNX1 locus pluripotent stem cells (hPSCs) to monitor and block expression gene during hPSC differentiation. This approach demonstrated that starts mesodermal specification focusing later on hemogenic endothelium (HE) nascent cells. Lack halted...
To improve the recapitulative quality of human pluripotent stem cell (hPSC) differentiation, we removed exogenous haematopoietic cytokines from defined differentiation system. Here, show that endogenous stimuli and VEGF are sufficient to induce robust hPSC-derived haematopoiesis, intensive generation progenitors, maturation blood cells emergence definitive precursor including those phenotypically identical early embryonic (HSCs). Moreover, cytokine-free system produces significantly higher...
MYB is a key regulator of definitive hematopoiesis and it dispensable for the development primitive hematopoietic cells in vertebrates. To delineate versus during differentiation human embryonic stem cells, we have introduced reporters into locus inactivated gene by bi-allelic targeting. recapitulate early developmental events more adequately, mutant wild type cell lines were differentiated defined culture conditions without addition cytokines. The reporter demonstrated that specifically...
MYB is a key regulator of definitive hematopoiesis that plays critical role in the maintenance and multilineage differentiation hematopoietic stem cells (HSCs). In vertebrate developmental models, thought to be dispensable for primitive hematopoiesis. To explore human development, we have subjected pluripotent (hPSCs) mono- bi-allelic gene targeting followed by defined culture conditions. Here show central development blood cells. expression was hematopoietic-specific its induction coincided...
RUNX1/AML1/CBFA2 (runt-related transcription factor 1/acute myeloid leukemia 1 protein/core-binding subunit alpha-2), is a that plays critical role in the development of normal hematopoiesis. RUNX1 also essential for immune cells and sensory neurons. Chromosomal translocations involving gene have been associated with several types leukemia. To investigate human hematopoietic we generated RUNX1-null embryonic stem cell reporter line GIBHe008-A by TALEN mediated homologous recombination. This...
Background: Osteogenesis imperfecta (OI) is a rare genetic disorder characterized by recurring bone fractures. Some OI patients have other clinical manifestations such as growth retardation, dental abnormalities, blue sclera, and hearing loss. The relationship between the phenotype genotype of indistinct, there no cure for OI. Therefore, an appropriate disease model urgently needed to understand pathophysiology Induced pluripotent stem cells (iPSCs) are capable developing into three germ...
MYB/c-MYB is a proto-oncogene encoding helix-turn-helix transcription factor that plays critical role in controlling proliferation and multilineage differentiation of hematopoietic progenitor stem cells. Deregulation MYB expression associated with several types leukemias lymphomas. In an attempt to explore the gene early human hematopoiesis, we have achieved bi-allelic targeting embryonic cells (hESCs) by TALEN-mediated homologous recombination. Furthermore, introduced eYFP Venus reporter...