A5052166596- Zebrafish Biomedical Research Applications
- Hematopoietic Stem Cell Transplantation
- Pluripotent Stem Cells Research
- Immune cells in cancer
- Acute Myeloid Leukemia Research
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- CAR-T cell therapy research
- Single-cell and spatial transcriptomics
- Cancer, Hypoxia, and Metabolism
- RNA Research and Splicing
- Hippo pathway signaling and YAP/TAZ
- Cytokine Signaling Pathways and Interactions
- Neuroinflammation and Neurodegeneration Mechanisms
- Genomics and Chromatin Dynamics
- TGF-β signaling in diseases
- Axon Guidance and Neuronal Signaling
- Cancer-related gene regulation
- Nanoplatforms for cancer theranostics
- Advanced Photocatalysis Techniques
- Phagocytosis and Immune Regulation
- Erythrocyte Function and Pathophysiology
- Biomedical Ethics and Regulation
- Neurogenetic and Muscular Disorders Research
Guangzhou Institutes of Biomedicine and Health
2012-2023
Chinese Academy of Sciences
2012-2023
V.I. Vernadsky Crimean Federal University
2023
Vavilov Institute of General Genetics
2021
University of Chinese Academy of Sciences
2019-2020
RIKEN
2007-2012
University of Edinburgh
2006
European Molecular Biology Laboratory
2005
Kyoto Prefectural University of Medicine
2005
Russian Academy of Sciences
1997
Microglia are the resident macrophages of central nervous system and associated with pathogenesis many neurodegenerative brain inflammatory diseases; however, origin adult microglia remains controversial. We show that postnatal hematopoietic progenitors do not significantly contribute to homeostasis in brain. In contrast macrophage populations, we develop mice lack colony stimulating factor-1 (CSF-1) but absent CSF-1 receptor-deficient mice. vivo lineage tracing studies established derive...
Langerhans cells (LCs) are the dendritic (DCs) of epidermis, forming one first hematopoietic lines defense against skin pathogens. In contrast to other DCs, LCs arise from precursors that seed before birth. However, origin these embryonic remains unclear. Using in vivo lineage tracing, we identify a wave yolk sac (YS)-derived primitive myeloid progenitors onset fetal liver hematopoiesis. YS migrate embryo proper, including prospective skin, where they give rise LC precursors, and brain...
The origin of the mammalian lymphatic vasculature has been debated for more than 100 years. Whether endothelial cells have a single or dual, venous mesenchymal remains controversial. To resolve this debate, we performed Cre/loxP -based lineage-tracing studies using mouse strains expressing Cre recombinase under control Tie2 , Runx1 Prox1 promoter elements. These studies, together with analysis -mutant embryos lacking definitive hematopoiesis, conclusively determined that from venous-derived...
Several lines of evidence suggest that the adult hematopoietic system has multiple developmental origins, but ontogenic relationship between nascent populations under this scheme is poorly understood. In an alternative theory, earliest definitive blood precursors arise from a single anatomical location, which constitutes cellular source for subsequent populations. To deconvolute ontogeny, we designed embryo-rescue in key factor Runx1 reactivated Runx1-null conceptuses at specific stages....
Nociceptors in peripheral ganglia display a remarkable functional heterogeneity. They can be divided into the following two major classes: peptidergic and nonpeptidergic neurons. Although RUNX1 has been shown to play pivotal role specification of neurons, mechanisms driving differentiation remain elusive. Here, we show that hepatocyte growth factor (HGF)-Met signaling acts synergistically with nerve factor-tyrosine kinase receptor A promote identity subset prospective nociceptors. We provide...
Mice deficient in the runt homology domain transcription factor Runx1 die of severe anemia utero by embryonic day (E)12.5. A reactivatable knockout stem cell (ESC) and mouse systems were generated targeted insertion a loxP-flanked multipartite gene stop/trap cassette designed to simultaneously ablate expression report on activity its promoters. The cassette's in-frame LacZ reporter enabled activities proximal distal promoters be differentially monitored. Although Runx1-null ESCs capable...
To improve the recapitulative quality of human pluripotent stem cell (hPSC) differentiation, we removed exogenous haematopoietic cytokines from defined differentiation system. Here, show that endogenous stimuli and VEGF are sufficient to induce robust hPSC-derived haematopoiesis, intensive generation progenitors, maturation blood cells emergence definitive precursor including those phenotypically identical early embryonic (HSCs). Moreover, cytokine-free system produces significantly higher...
MYB is a key regulator of definitive hematopoiesis and it dispensable for the development primitive hematopoietic cells in vertebrates. To delineate versus during differentiation human embryonic stem cells, we have introduced reporters into locus inactivated gene by bi-allelic targeting. recapitulate early developmental events more adequately, mutant wild type cell lines were differentiated defined culture conditions without addition cytokines. The reporter demonstrated that specifically...
Abstract Non‐receptor tyrosine kinase Chk has been implicated in hematopoietic development. To study the function of vivo, we have generated chk‐deficient mice using gene targeting. Overall development homozygous for this mutation was apparently normal. Blood counts, FACS analysis cell populations, CFU‐C and CAFC assays showed no significant difference between wild type mutant animals. Thus, dispensability mouse hematopoiesis suggests that its may be redundant most likely compensated by...
кО-кУЛЬтИВИРОВАНИЕ С РАкОВЫмИ кЛЕткАмИ АктИВИРУЕт ДЕНДРИтНЫЕ кЛЕткИ, пОЛУЧЕННЫЕ ИЗ пЛЮРИпОтЕНтНЫХ СтВОЛОВЫХ кЛЕтОк ЧЕЛОВЕкА И. м.Самохвалов 1, 2 , к. Д. малый 1 Е. С. Агеева Э. т.Дегирменджи А. к.Гуртовая Р. Н. Аблаева п. трофимов В. кубышкин медицинский институт им.С. Георгиевского, крымский федеральный университет им.В. Вернадского, Симферополь, Российская Федерация Инжиниринговый центр генетических и клеточных биотехнологий, им
MYB is a key regulator of definitive hematopoiesis that plays critical role in the maintenance and multilineage differentiation hematopoietic stem cells (HSCs). In vertebrate developmental models, thought to be dispensable for primitive hematopoiesis. To explore human development, we have subjected pluripotent (hPSCs) mono- bi-allelic gene targeting followed by defined culture conditions. Here show central development blood cells. expression was hematopoietic-specific its induction coincided...
RUNX1/AML1/CBFA2 (runt-related transcription factor 1/acute myeloid leukemia 1 protein/core-binding subunit alpha-2), is a that plays critical role in the development of normal hematopoiesis. RUNX1 also essential for immune cells and sensory neurons. Chromosomal translocations involving gene have been associated with several types leukemia. To investigate human hematopoietic we generated RUNX1-null embryonic stem cell reporter line GIBHe008-A by TALEN mediated homologous recombination. This...
Systemic lupus erythematosus (SLE) is a heterogeneous, autoimmune disease that can affect multiple organs and systems such as skin, joints, kidneys, hematologic system or central nervous system. Women of childbearing age are the predominate population affected by SLE. In this study, we generated an iPS cell line from 30-year-old female who was pregnant with gestational 27 weeks diagnosed severe preeclampsia, SLE psoriasis. This patient-specific iPSC will be useful to create specific model...
Recently, we and others have cloned cDNAs encoding a second member of the Csk family inhibitory protein kinases, which termed Bhk [M.A. Ershler et al. (1994) Dokl. Akad. Nauk. 339, 679–683]. In present study, two new distinct types bhk mRNA were found in addition to third form described previously. Analysis genomic structure established that three exons participate alternative splicing mRNA.
Background: Osteogenesis imperfecta (OI) is a rare genetic disorder characterized by recurring bone fractures. Some OI patients have other clinical manifestations such as growth retardation, dental abnormalities, blue sclera, and hearing loss. The relationship between the phenotype genotype of indistinct, there no cure for OI. Therefore, an appropriate disease model urgently needed to understand pathophysiology Induced pluripotent stem cells (iPSCs) are capable developing into three germ...