In this study, we report the cloning and expression of lipase gene from Pseudomonas fluorescens B52, a psychrotrophic spoilage bacterium isolated refrigerated raw milk. Sequence analysis revealed one major open reading frame 1,428 nucleotides that was predicted to encode protein with molecular weight 50,241. The enzyme found contain an amino acid sequence highly homologous putative substrate-binding domain present within all lipases examined date.

The GP64 and F proteins were previously identified as the sole functional envelope fusion in Baculoviridae. F-like proteins, present only group I nucleopolyhedroviruses (NPVs), are remnant, nonfunctional proteins. In this report, we describe effect of presence or absence protein Ac23 a gp64-null Autographa californica multinucleocapsid NPV pseudotyped with from Spodoptera exigua multicapsid (SeF). We found that elevates infectivity virus. This is contrast to results Lung et al. (J. Virol....

The entry of baculovirus budded virus into host cells is mediated by two distinct types envelope fusion proteins (EFPs), GP64 and F protein. Phylogenetic analysis suggested that were ancestral EFPs, whereas was acquired progenitor group I alphabaculovirus more recently may have stimulated the formation lineage. This study designed to experimentally recapitulate a possible major step in evolution baculoviruses. We demonstrated infectivity an F-null II (Helicoverpa armigera...

Aminobenzotriazole (ABT) is commonly used as a non-selective inhibitor of cytochrome P450 (CYP) enzymes to assign contributions CYP versus non-CYP pathways the metabolism new chemical entities. Despite widespread use, systematic review drug-drug interaction (DDI) potential for ABT has not been published nor have implications using it in plated hepatocyte models low clearance reaction phenotyping. The goal being investigate utility pan-CYP phenotyping compounds by evaluating stability over...

ABSTRACT Two distinct envelope fusion proteins (EFPs) (GP64 and F) have been identified in members of the Baculoviridae family viruses. F are found group II nucleopolyhedroviruses (NPVs) alphabaculoviruses beta- deltabaculoviruses, while GP64 occurs only I NPVs alphabaculoviruses. It was proposed that an ancestral baculovirus acquired gp64 gene conferred a selective advantage allowed it to evolve into NPVs. The protein is functional analogue GP64, as evidenced from rescue -null Autographa...

To cope with unrepaired DNA lesions, cells are equipped damage tolerance mechanisms, including translesion synthesis (TLS). While TLS polymerases well documented in facilitating replication across damaged templates, it remains unknown whether participate transcriptional bypass of lesions cells. Herein, we employed the competitive transcription and adduct assay to examine efficiencies fidelities N2-alkyl-2′-deoxyguanosine (N2-alkyl-dG, alkyl = methyl, ethyl, n-propyl, or n-butyl) HEK293T We...

The envelope fusion protein F of Plutella xylostella granulovirus is a computational analogue the GP64 Autographa californica nucleopolyhedrovirus (AcMNPV). Granulovirus (GV) proteins were thought to be unable functionally replace in AcMNPV pseudotyping system. In present study Agrotis segetum GV (AgseGV) was identified experimentally as first functional from GVs. AgseF can rescue virion propagation and infectivity gp64-null AcMNPV. AgseF-pseudotyped also induced syncytium formation...

F proteins of group II nucleopolyhedroviruses (NPVs) are envelope fusion essential for virus entry and egress. An F-null Helicoverpa armigera single nucleocapsid NPV (HearNPV) bacmid, HaBacDeltaF, was constructed. This bacmid could not produce infectious budded (BV) when transfected into HzAM1 cells, showing that protein is cell-to-cell transmission BVs. When HaBacDeltaF pseudotyped with the homologous (HaBacDeltaF-HaF, positive control) or heterologous from Spodoptera exigua...

Quantitative measurement of DNA adducts in carcinogen-exposed cells provides the information about frequency formation and rate removal lesions vivo, which yields insights into initial events mutagenesis. Metabolic activation tobacco-specific nitrosamines, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) its reduction product 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL), leads to pyridyloxobutylation pyridylhydroxybutylation DNA. In this study, we employed a highly robust...

2,6-Diaminopurine (Z) is a naturally occurring adenine (A) analog that bacteriophages employ in place of A their genetic alphabet. Recent discoveries biogenesis pathways Z have stimulated substantial research interest this DNA modification. Here, we systematically examined the effects on efficiency and fidelity transcription. Our results showed exhibited no mutagenic yet inhibitory transcription mediated by purified T7 RNA polymerase human II HeLa nuclear extracts cells. structurally related...

Understanding the occurrence, repair, and biological consequences of DNA damage is important in environmental toxicology risk assessment. The most common way to assess elicited by exogenous sources a laboratory setting expose cells or experimental animals with chemicals that modify DNA. Owing lack reaction specificities damaging agents, approach frequently does not allow for induction specific lesion. Herein, we employed metabolic labeling selectively incorporate N2-methyl-dG (N2-MedG)...

Environmental, endogenous and therapeutic alkylating agents can react with internucleotide phosphate groups in DNA to yield alkyl phosphotriester (PTE) adducts. Alkyl-PTEs are induced at relatively high frequencies persistent mammalian tissues; however, their biological consequences cells have not been examined. Herein, we assessed how alkyl-PTEs different group sizes stereochemical configurations (SP RP diastereomers of Me nPr) affect the efficiency fidelity transcription cells. We found...

Binary expression systems have revolutionised genetic research by enabling delivery of loss-of-function and gain-of-function transgenes with precise spatial-temporal resolution in vivo. However, at present, each existing platform relies on a defined exogenous transcription activator capable binding unique recognition sequence. Consequently, none these technologies alone can be used to simultaneously target different tissues or cell types the same organism. Here, we report modular system...

ABSTRACT The entry of enveloped viruses into cells is normally mediated by fusion between viral and cellular membranes, in which the peptide plays a crucial role. peptides group II nucleopolyhedrovirus (NPV) F proteins are quite conserved, with hydrophobic region located at N terminal 1 fragment. For this report, we used mutagenesis nuclear magnetic resonance (NMR) to study structure function Helicoverpa armigera single-nucleocapsid NPV (HearNPV) protein (HaF). Five mutations HaF, G, L, I 2...

Recent advances in bipartite consensus on matrix-weighted networks, where agents are divided into two disjoint sets with those the same set agreeing a certain value and different converging to opposite values, have highlighted its potential applications across various fields. Traditional approaches often depend existence of positive-negative spanning tree networks achieve consensus, which greatly restricts use these engineering applications. This study relaxes that assumption by allowing...

Puberulic acid (PA) is a mycotoxin produced by species of Penicillium. It has received widespread attention as significant contributor to the reported fatalities associated with red yeast rice dietary supplements. However, detection PA, especially at low concentration levels, poses considerable challenge, no methods thus far. Here, we present simple and sensitive derivatization-based LC-MS/MS method, requiring purification processes, for determination PA in rice. The methylating...

The major envelope fusion protein F of the budded virus baculoviruses consists two disulfide-linked subunits: an N-terminal 2 subunit and a C-terminal, membrane-anchored 1 subunit. There is one cysteine in there are 15 cysteines , but their role disulfide linking largely unknown. In this study, inter- intra-subunit bonds Helicoverpa armigera single nucleocapsid nucleopolyhedrovirus (HearNPV) were analysed by site-directed mutagenesis. Results indicated that functional protein, inter-subunit...


 Objectives: The popularity of organic diets continues to increase even without sound evidence that these are healthier than conventional diets. As its increases, foods become more readily available and accessible the public, genuinity comes into question as farmers retailers find ways profit from this trend. Although produce will never be completely free pesticides, they expected have considerably lower amounts. In recent years, pesticide residues in apples been found at levels higher...

Objective To construct and identify replication deficient recombinant adenovirus expressing human c-Jun N-terminal kinase(JNK)by homologous recombination dominant-negative type JNK(Ad-DN-JNK).Methods The linearized shuttle vector pAdTrack-CMV-DN-JNK Was co-transformed with backbone pAdEasy-l into bacteria BJ5183 for adenoviral vector.The was transfected HEK293 packing cells tO adenovirus,and then the edenovirns WaS detected by PCR DNA sequencing.Western blot analysis utilized to detect...