The current status of multi-mycotoxin contamination in edible and medicinal plants demands urgent development high-throughput analytical methods for mycotoxin detection. In this study, a reliable sensitive method the simultaneous analysis 73 mycotoxins was established successfully applied to detect 260 samples four dual-purpose (lotus seed, coix licorice root, dried tangerine peel). Sample preparation involved optimized QuEChERS (Quick, Easy, Cheap, Effective, Rugged, Safe) extraction...

Red yeast rice is a nutraceutical fermented product used worldwide for the symptomatic relief of dyslipidemia and cardiovascular disease. However, fermentation-induced lipid transformation from to red remains unclear. Herein, an ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry method was developed comprehensive analysis during fermentation. A total 246 lipids fall in 21 subclasses were annotated rice, including 37 lysophospholipids, 14...

Cadmium (Cd), a heavy metal element has strong toxicity to living organisms. Excessive Cd accumulation directly affects the absorption of mineral elements, inhibits plant tissue development, and even induces mortality. Populus × canadensis 'Neva', main afforestation variety planted widely in northern China, was candidate for phytoremediation. However, genes relieving increasing tolerance this species were still unclear. In study, we employed transcriptome sequencing on two Cd–treated...

In this study, a residue analysis method for the simultaneous determination of 107 pesticides in traditional Chinese medicines (TCMs), Angelica sinensis, A. dahurica, Leonurus heterophyllus Sweet, Pogostemon cablin and Lonicera japonica Thunb., was developed using gas chromatography coupled with tandem mass spectrometry negative chemical ionisation mode (GC-NCI-MS/MS). NCI has advantages high sensitivity selectivity to chemicals electron-withdrawing groups, yields low background...

Abstract Ferroptosis is a novel form of iron‐dependent cell death that involved in arsenic‐induced toxicity. Realgar an arsenic‐containing Chinese medicine, which can result nephrotoxicity because long‐term exposure. However, it remains scientifically unknown whether inducer ferroptosis the kidney. This study investigated role Realgar‐induced kidney toxicity mice. ICR mice were exposed to for 28 days, and HK2 cells presence or absence treatment with ferrostatin‐1, inhibitor. The...

PIN-FORMED (PIN) proteins, which function as efflux transporters, play many crucial roles in the polar transportation of auxin within plants. In this study, exogenous applications IAA and TIBA were found to significantly prolong shorten florescence tree peony (Paeonia suffruticosa Andr.) flowers. This finding suggests that has some regulatory influence petal senescence abscission. Further analysis revealed a total 8 PsPINs distributed across three chromosomes, could be categorized into two...

Continuous veno-venous hemofiltration (CVVH) in the treatment of severe acute pancreatitis (SAP) was investigated. A total 60 patients with SAP treated Affiliated Dongtai Hospital Nantong University from October 2015 to 2017 were enrolled this retrospective study. Among them, 32 received CVVH and routine internal medicine therapy (the group), 28 control group). Then biochemical indexes severity after detected investigate roles treating SAP. Inflammatory factors both groups significantly...

The present study described the development and validation of a sensitive selective analytical method for simultaneous determination 23 triazines in herbal plants. Pesticides were extracted using modified QuEChERS procedure, extracts cleaned up with gel permeation chromatography (GPC) molecularly imprinted solid-phase extraction (MISPE) followed by UHPLC-MS/MS. polymers (MIPs) prepared traditional precipitation N-butylmelamine as template methacrylic acid (MAA) functional monomer. linearity...

Realgar has been used as a type of mineral drug that contains arsenic for thousands years. Previous studies have shown Realgar-induced acute kidney injury is associated with abnormal metabolism, but the underlying mechanism poorly understood. The aim this study to investigate metabolic changes in serum and tissues mice exposed by using metabolomic approach explore molecular mechanisms induced Realgar. Forty were randomly divided into four groups: Control group, 0.5-, 1.0, 2.0 g/kg group....

Recently, serious concerns have been raised about potential safety risk posed by exogenous pesticide contaminants in herbal medicines. It is highly desirable to reliable detection and determination of suspected residues for understanding their overall quality. However, inherent metabolites abundant medicines typically make screening trace-level arduous. Taking Lonicerae Japonicae Flos (LJF) as an example, the current study investigated capability a three-dimensional separation system, ultra...

Platycodon root, a medicinal food homology species which has been used in Asian countries for hundreds of years, is now widely cultivated China. Treatment with paclobutrazol, typical plant growth retardant, raised uncertainties regarding the quality have rarely investigated. In present study, metabolomic and lipidomic differences were revealed by ultra-high performance liquid chromatography coupled to ion mobility-quadrupole time flight mass spectrometry (UPLC-IM-QTOF-MS). A significant...

The presence of illegally adulterated sildenafil and related compounds in dietary supplements was qualitatively analyzed by employing ultra-high performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS). Samples were extracted ultrasonic method using methanol. analysis performed on an Agilent Poroshell 120 SB-C18 column (75 mm×3.0 mm, 2.7 μm) gradient elution with a mixture aqueous formic acid solution (0.1%, v/v) acetonitrile as the mobile phase at flow...

Platycodon grandiflorus (Jacq.) A. DC. is widely cultivated across the south and north of China. Its root, Platycodonis radix, commonly used as a vegetable, functional food, traditional herbal medicine with various biological benefits. It critical to fully clarify chemical composition radix for sake food industry herb markets. In this study, strategy metabolome lipidome profiling based on ultra-high performance liquid chromatography coupled ion mobility-quadrupole time flight mass...

Quality control of animal-derived traditional Chinese medicines has improved dramatically as proteomics research advanced in the past few decades. However, it remains challenging to identify quality attributes with routine approaches since protein fibrinolytic activity is rarely reported pheretima, a typical medicine. A novel strategy based on bioinformatics combined parallel reaction monitoring (PRM) was developed here rapidly discover marker peptides associated effect. Potential were found...