A5100351209- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Advanced biosensing and bioanalysis techniques
- Chemical Synthesis and Analysis
- RNA Interference and Gene Delivery
- Monoclonal and Polyclonal Antibodies Research
- Signaling Pathways in Disease
- Electrospun Nanofibers in Biomedical Applications
- MicroRNA in disease regulation
- Neurogenesis and neuroplasticity mechanisms
- Hydrogels: synthesis, properties, applications
- Click Chemistry and Applications
- Crystallography and molecular interactions
- Photochromic and Fluorescence Chemistry
- Polymer Surface Interaction Studies
- Cancer-related molecular mechanisms research
- 3D Printing in Biomedical Research
- Peptidase Inhibition and Analysis
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA and protein synthesis mechanisms
- Photoreceptor and optogenetics research
- RNA modifications and cancer
- Innovative Microfluidic and Catalytic Techniques Innovation
- Reconstructive Surgery and Microvascular Techniques
- Antibiotic Resistance in Bacteria
Huashan Hospital
2021-2025
Fudan University
2021-2025
Shanghai Ninth People's Hospital
2008-2025
State Key Laboratory of Oral Diseases
2017-2025
Peking University People's Hospital
2025
Sichuan University
2014-2025
Chinese Academy of Medical Sciences & Peking Union Medical College
2024-2025
Xijing Hospital
2025
Air Force Medical University
2025
Dalian University of Technology
2015-2025
FOXO1A and FOXO3A are two members of the FoxO family. has recently been linked to human longevity in Japanese, German Italian populations. Here we tested genetic contribution phenotype Han Chinese population. Six tagging SNPs from were selected genotyped 1817 centenarians younger individuals. Two found be associated with women (P = 0.01–0.005), whereas all three both genders 0.005–0.001). One SNP was not longevity. In haplotype association tests, OR (95% CI) for haplotypes TTG CCG female...
Periodontal ligament stem cells (PDLSCs) are considered as candidate for the regeneration of periodontal and alveolar bone tissues. Antisense to cerebellar degeneration-related protein 1 transcript (CDR1as), which is a newly discovered circular RNA (circRNA), has been reported act an miR-7 sponge be involved in many biological processes. Here, we investigated potential roles CDR1as osteogenic differentiation PDLSCs. The expression pattern PDLSCs during osteogenesis was detected by...
Abstract Many features of extracellular matrices, e.g., self-healing, adhesiveness, viscoelasticity, and conductivity, are associated with the intricate networks composed many different covalent non-covalent chemical bonds. Whereas a reductionism approach would have limitation to fully recapitulate various biological properties simple structures, mimicking such sophisticated by incorporating functional groups in macromolecular system is synthetically challenging. Herein, we propose strategy...
The conversion of excess carbon dioxide (CO2) into valuable chemicals is critical for achieving a sustainable society. Among various catalysts, molybdenum disulfide (MoS2) has demonstrated potential CO2 hydrogenation to methanol. However, its catalytic activity yet be fully optimized, and scalable, industrially viable production methods remain underdeveloped. In this work, chemical vapor deposition (CVD) approach introduced grow vertically oriented MoS2 crystals on an amorphous template....
SummaryBackgroundCarbapenem-resistant hypervirulent Klebsiella pneumoniae (CR-hvKp) has been increasingly reported worldwide, posing a severe challenge to public health; however, the mechanisms driving its emergence and global dissemination remain unclear.MethodsWe analysed CR-hvKp strains derived from canonical hvKp backgrounds, acquired carbapenemase-encoding gene. These were identified 485 CRKp isolates in CRACKLE-2 China cohort, 259 multi-centre study, 67,631 K. genomes available...
DNA encoding facilitates the construction and screening of large chemical libraries. Here, we describe general strategies for stepwise coupling coding fragments to nascent organic molecules throughout individual reaction steps as well first implementation high-throughput sequencing identification relative quantification library members. The methodology was exemplified in a DNA-encoded containing 4,000 compounds discovery binders streptavidin, matrix metalloproteinase 3, polyclonal human IgG.
Seeing eye to eye: Plasma-protein binding is effective in improving the pharmacokinetic properties of otherwise short-lived molecules. One compound a class small portable albumin binders can be used improve vivo circulatory half-life two widely contrast agents. It improves imaging performance fluorescein angiographic analysis retina mice (see picture). Supporting information for this article available on WWW under http://www.wiley-vch.de/contents/jc_2002/2008/z704936_s.pdf or from author....
NaYbF4:RE upconversion (UC) fluorescent nanoparticles (NPs) were synthesized with variable rare-earth dopants (RE = Er3+, Tm3+, or Ho3+, a combination of these ions) from stearate precursors in water−ethanol−oleic acid system by using two-phase solvothermal method. The NPs shown to emit visible light, such as orange, yellow, green, cyan, blue pink light response near-infrared (NIR) irradiation, and their emission colors could be simply tuned changing either the codopant concentration dopant...
The miR-34 family members are direct transcriptional targets of tumor suppressor p53, and loss function can impair p53-mediated cell cycle arrest apoptosis. A potentially functional SNP rs4938723 (T > C) was found in the promoter region pri-miR-34b/c (423 bp from transcription start site), located CpG island might affect factor GATA binding therefore expression. In our study, we hypothesized that SNPs miR-34b/c TP53 Arg72Pro may independently or jointly contribute to primary hepatocellular...
Human brains are prone to neurodegeneration, given that endogenous neural stem/progenitor cells (NSPCs) fail support neurogenesis. To investigate the molecular programs potentially mediating neurodegeneration-induced NSPC plasticity in regenerating organisms, we generated an Amyloid-β42 (Aβ42)-dependent neurotoxic model adult zebrafish brain through cerebroventricular microinjection of cell-penetrating Aβ42 derivatives. deposits neurons and causes phenotypes reminiscent amyloid...
Abstract We report a synthetic biology‐inspired approach for the engineering of amphipathic DNA origami structures as membrane‐scaffolding tools. The have flat membrane‐binding interface decorated with cholesterol‐derived anchors. Sticky oligonucleotide overhangs on their side facets enable lateral interactions leading to formation ordered arrays membrane. Such tight and regular arrangement makes our capable deforming free‐standing lipid membranes, mimicking biological activity coat‐forming...
The neural stem cell (NSC) reservoir can be harnessed for cell-based regenerative therapies. Zebrafish remarkably regenerate their brain by inducing NSC plasticity in a Amyloid-β-42 (Aβ42)-induced experimental Alzheimer's disease (AD) model. Interleukin-4 (IL-4) is also critical AD-induced proliferation. However, the mechanisms of this response have remained unknown. Using single-cell transcriptomics adult zebrafish brain, we identify distinct subtypes NSCs and neurons differentially...
Abstract Libraries of chemical compounds individually coupled to encoding DNA tags (DNA‐encoded libraries) hold promise facilitate exceptionally efficient ligand discovery. We constructed a high‐quality DNA‐encoded library comprising 30 000 drug‐like compounds; this was screened in 170 different affinity capture experiments. High‐throughput sequencing allowed the evaluation 120 million codes for systematic analysis selection strategies and statistically robust identification binding...
Abstract Synthetic conductive biopolymers have gained increasing interest in tissue engineering, as they can provide a chemically defined electroconductive and biomimetic microenvironment for cells. In addition to low cytotoxicity high biocompatibility, injectability adhesiveness are important many biomedical applications but proven be very challenging. Recent results show that fascinating material properties realized with bioinspired hybrid network, especially through the synergy between...
It was recently suggested that supplying the brain with new neurons could counteract Alzheimer's disease (AD). This provocative idea requires further testing in experimental models which molecular basis of disease-induced neuronal regeneration be investigated. We previously found zebrafish stimulates neural stem cell (NSC) plasticity and neurogenesis AD help to understand mechanisms harnessed for developing diseased mammalian brains. Here, by performing single-cell transcriptomics, we...
The study of cells responding to an electroconductive environment is impeded by the lack a method, which would allow encapsulation in extracellular matrix-like 3D electroactive matrix, and more challengingly, permit simple mechanism release for further characterization. Herein, we report polysaccharide-based conductive hydrogel system formed via β-cyclodextrin-adamantane host-guest interaction. Oxidative polymerization 3,4-ethylenedioxythiophene (EDOT) presence adamantyl-modified sulfated...
Abstract Electrically conductive materials that mimic physical and biological properties of tissues are urgently required for seamless brain–machine interfaces. Here, a multinetwork hydrogel combining electrical conductivity 26 S m −1 , stretchability 800%, tissue‐like elastic modulus 15 kPa with mimicry the extracellular matrix is reported. Engineering this unique set enabled by novel in‐scaffold polymerization approach. Colloidal hydrogels nanoclay Laponite employed as supports assembly...
This study set out to assess the mitigative effects of curcumin on AFB1-induced necroptosis and inflammation in chicken liver. Ninety-six one-day-old AA broiler chickens were separated into four groups, including control group, AFB1 (1 mg/kg) (300 + group group. After 28 days treatment, livers collected for different experimental analyses. The morphological observation results showed obvious necrotic characteristics, cell swelling, rupture mitochondrial membranes livers. exposure increased...
Abstract Functional segmental trachea reconstruction remains a remarkable challenge in the clinic. To date, functional regeneration with alternant cartilage‐fibrous tissue‐mimetic structure similar to that of native relying on three‐dimensional (3D) bioprinting technology has seen very limited breakthrough. This fact is mostly due lack tissue‐specific bioinks suitable for both cartilage and vascularized fibrous tissue regeneration, as well need firm interfacial integration between stiff soft...