A5066333550- Monoclonal and Polyclonal Antibodies Research
- Proteoglycans and glycosaminoglycans research
- 3D Printing in Biomedical Research
- Hydrogels: synthesis, properties, applications
- Electrospun Nanofibers in Biomedical Applications
- Glycosylation and Glycoproteins Research
- Viral Infectious Diseases and Gene Expression in Insects
- Supramolecular Self-Assembly in Materials
- Silk-based biomaterials and applications
- Biochemical and Structural Characterization
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Cellular Mechanics and Interactions
- Additive Manufacturing and 3D Printing Technologies
- Signaling Pathways in Disease
- Polymer Surface Interaction Studies
- Receptor Mechanisms and Signaling
- Mass Spectrometry Techniques and Applications
- Plant Pathogenic Bacteria Studies
- Bone Tissue Engineering Materials
- Cell Adhesion Molecules Research
- Toxin Mechanisms and Immunotoxins
- Connective tissue disorders research
- Advanced Biosensing Techniques and Applications
- Protein purification and stability
TU Dresden
2013-2022
Leipzig University
2019-2021
University of Oxford
2018
Martin Luther University Halle-Wittenberg
2009
Abstract Synthetic conductive biopolymers have gained increasing interest in tissue engineering, as they can provide a chemically defined electroconductive and biomimetic microenvironment for cells. In addition to low cytotoxicity high biocompatibility, injectability adhesiveness are important many biomedical applications but proven be very challenging. Recent results show that fascinating material properties realized with bioinspired hybrid network, especially through the synergy between...
The Gram-negative phytopathogenic bacterium Xanthomonas campestris pv. vesicatoria (Xcv) employs a type III secretion system to translocate effector proteins into plant cells where they modulate host signaling pathways the pathogen's benefit. protein AvrBs3 acts as eukaryotic transcription factor and induces expression of genes termed UPA (up-regulated by AvrBs3). Here, we describe 11 new from bell pepper that are induced early after infection with Xcv. Sequence comparisons revealed presence...
Reduction of complexity the extracellular matrix (ECM) to a non-covalent structure with minimal chemically defined components represents an attractive avenue for understanding biology ECM. The resulting system could lead design tailor-made biomaterials that incorporate varying functionalities. Negatively charged glycosaminoglycans are major Their interaction positively proteins is important dynamic three-dimensional scaffold formation and function. We designed screened peptide motifs whose...
Hydrogel beads as microcarriers could have many applications in biotechnology. However, bead formation by noncovalent cross-linking to achieve high cell compatibility avoiding chemical reactions remains challenging because of rapid gelation rates and/or low stability. Here we report the preparation homogeneous, tunable, and robust hydrogel from peptide-polyethylene glycol conjugates oligosaccharides under mild, cell-compatible conditions using a crosslinking mechanism. Large proteins can be...
Hydrogels can serve as matrices to mimic natural tissue function and be used for wide‐ranging applications such regeneration drug delivery. Injectable hydrogels are particularly favorable because their uses minimally invasive. However, creating moldable substance injection often results in compromised stability. This study reports an injectable hydrogel system crosslinked by peptide–oligosaccharide noncovalent interaction. The dynamic network shows fast self‐healing, a property essential...
Abstract Interactions with the extracellular matrix (ECM) dictate cell fates. However, complexity of dense ECM network and cell-surface molecules prevent study their dynamic interaction at molecular level on living cells. Here, we focus peptidyl prolyl cis/trans isomerases (PPIases) to dissect isomerization from other events. We reveal contribution PPIase mechanical properties various materials cell–ECM interaction. To avoid complications associated existing spectroscopy-based methods such...
A repertoire of conjugable tags for controlling the release drugs from biomaterials is highly interesting development combinatorial drug administration techniques. This paper describes such a system 11 peptide derived our previous work on physical hydrogel cross-linked through peptide-heparin interactions. The kinetics correlate well with their affinity to heparin and obey Fick's second law diffusion, exception ATIII peptide, which displays stable profile close zero-order reaction....
Abstract Combinatorial screening represents a promising strategy to discover biomaterials for tailored cell culture applications. Although libraries incorporating different biochemical cues have been investigated, few simultaneously recapitulate relevant biochemical, physical, and dynamic features of the extracellular matrix (ECM). Here, noncovalent system based on liquid–liquid phase separation (coacervation) gelation mediated by glycosaminoglycan (GAG)‐peptide interactions is reported....
Reducing the complex extracellular matrices (ECM) to chemically defined polymer networks represents an attractive avenue for understanding and recapitulating biology of ECM. However, while lipids are also important components ECM, it remains challenging investigate as a modular functionality in synthetic biomaterials, especially self-assembled network. In this study, order establish amphiphilic system, lipid-modifications introduced into noncovalent hydrogel system cross-linked by...
We investigated the utility of a heparin/peptide-polyethylene glycol conjugate system to build layer-by-layer (LbL) structures, assemble tailored multilayer-biomatrices for cell culture. The LbL assembly balances advantages polyelectrolyte systems and protein-based systems. Human umbilical vein endothelial cells showed distinct responses film thickness structure; presence, density, spatial arrangement adhesion ligand within nanothin film; pretreatment with morphogens. technique presents...
Abstract Hydrogel beads as microcarriers could have many applications in biotechnology. However, bead formation by noncovalent cross‐linking to achieve high cell compatibility avoiding chemical reactions remains challenging because of rapid gelation rates and/or low stability. Here we report the preparation homogeneous, tunable, and robust hydrogel from peptide–polyethylene glycol conjugates oligosaccharides under mild, cell‐compatible conditions using a crosslinking mechanism. Large...
Abstract Stem cell bioengineering and therapy require different model systems materials in stages of development. If a chemically defined biomatrix system can fulfill most tasks, it minimize the discrepancy among various setups. By screening biomaterials synthesized through coacervation‐mediated self‐assembling mechanism, optimal for 2D human mesenchymal stromal (hMSC) culture osteogenesis is identified. Its utility hMSC further demonstrated coating porous bioactive glass scaffolds...
In Abbildung 2 c dieser Zuschrift wurde die horizontale Achse fälschlicherweise mit “t/h” anstelle richtig “t/min” beschriftet. Die korrigierte Version von 1 ist daher hier gezeigt. Autoren bitten, diesen Fehler zu entschuldigen. […] c) Release of fluorescence-labeled IgG antibodies from hydrogel beads (10 mg mL−1).