This study set out to assess the mitigative effects of curcumin on AFB1-induced necroptosis and inflammation in chicken liver. Ninety-six one-day-old AA broiler chickens were separated into four groups, including control group, AFB1 (1 mg/kg) (300 + group group. After 28 days treatment, livers collected for different experimental analyses. The morphological observation results showed obvious necrotic characteristics, cell swelling, rupture mitochondrial membranes livers. exposure increased...

Abstract Sepsis-induced acute lung injury (ALI) is a serious sepsis complication and the prevailing cause of death. Circulating plasma exosomes might exert key role in regulating intercellular communication between immunological structural cells, as well contributing to sepsis-related organ damage. However, molecular mechanisms by which exosome-mediated signaling exacerbate ALI septic infection remains undefined. Therefore, we investigated effect macrophage-derived exosomal APN/CD13 on...

Mitophagy is involved in sepsis-induced acute lung injury (ALI). Bcl-2 family proteins play an important role mitochondrial homeostasis. However, whether targeting (Bcl-2 and Bad) could influence mitophagy ALI remains unclear. In this study, lipopolysaccharide (LPS) was used to induce A549 cells mice. LPS treatment resulted elevated cell apoptosis, enhanced mitophagy, decreased expression, increased Bad activation of PINK1/Parkin signaling tissues. Both overexpression knockdown attenuated...

Aflatoxin B1 (AFB1) is a potent hepatotoxic and carcinogenic agent. Curcumin possesses potential anti-inflammatory, anti-oxidative hepatoprotective effects. However, the role of LncRNAs in protective mechanisms curcumin against AFB1-induced liver damage still elusive. Experimental broilers were randomly divided into 1) control group, 2) AFB1 group (1 mg/kg feed), 3) cur + plus 300 diet) 4) (300 diet). Liver transcriptome analyses qPCR performed to identify shifts genes expression. In...

There are increasing evidence that left ventricle diastolic dysfunction is the initial functional alteration in diabetic myocardium. In this study, we hypothesized alogliptin prevents and preserves ventricular mitochondrial function structure rabbits. A total of 30 rabbits were randomized into control group (CON, n = 10), alloxan-induced (DM, 10) alogliptin-treated (12.5 mg/kd/day for 12 weeks) (DM-A, 10). Echocardiographic hemodynamic studies performed vivo. Mitochondrial morphology,...

Mitophagy removes dysfunctional mitochondria and is known to play an important role in the pathogenesis of several diseases; however, mitophagy acute respiratory distress syndrome (ARDS) remains poorly understood. While we have previously demonstrated that polydatin (PD) improves lipopolysaccharide (LPS)-induced ARDS, specific mechanism unclear. In present study, aimed determine whether PD activates Parkin-dependent protect against LPS-induced mitochondria-dependent apoptosis lung injury. To...

In this study, we examined the protective effects of curcumin against AFB1-induced immune response and pathological changes in broilers. Histopathology examinations showed that at day 28, AFB1 (5 mg/kg) exposure leads to severe histological spleen, thymus bursa Fabricius with a decrease number karyoplasmic area ratio plasma cells. Curcumin alleviated organs’ damage as well cells dose-dependent manner. RT-PCR data significantly downregulated IL-2 IFN-γ mRNA expression levels thymus, spleen...

Colistin is the last line of defense for treatment multidrug-resistant gram-negative bacterial infections. However, colistin resistance gradually increasing worldwide, with commonly regulated by two-component system and mcr gene. Thus, this study aimed to investigate molecular epidemiology colistin-resistant mechanism mcr-positive mcr-negative Escherichia coli isolates from animal in Sichuan Province, China. In study, a total 101 E. strains were isolated 300 fecal samples six farms Province....

Recent evidence indicates that peroxisome proliferator-activated receptor (PPAR)-γ activators exert anti-inflammatory and antioxidant actions. However, the underlying mechanisms by which these agents prevent atrial remodeling in diabetes are not completely elucidated. We sought to investigate potential effects of pioglitazone, a PPAR-γ activator, on fibrillation (AF) inducibility diabetic rabbits.Alloxan-induced rabbits were randomly divided into three groups: only, treated with low-dose...

The regulation of intracellular mitochondria degradation is mediated by mitophagy. While studies have shown that mitophagy can lead to mitochondrial dysfunction and cell damage, the role Mdivi-1 remains unclear in acute lung injury (ALI) pathogenesis. In this study, we demonstrated Mdivi-1, which widely used as an inhibitor mitophagy, ameliorated assessed HE staining, pulmonary microvascular permeability assay, measurement wet/dry weight (W/D) ratio, oxygenation index (PaO2/FiO2) analysis....

The purpose of this study was to investigate the role epigenetic DNA methylation and CYPs expression in AFB1-exposed broiler liver protective effect curcumin. Sixty-four one-day-old AA broilers were randomly divided into four groups, including control group, AFB1 group (1 mg/kg AFB1), curcumin + curcumin) (300 curcumin). Histological observation, CYP450 enzyme activities, levels methyltransferases enzymes, overall level investigated. Dietary found induce severe injury broilers, upregulate...

Aflatoxin B1 (AFB1) is extremely carcinogenic and can cause liver cancer in humans animals with continued ingestion. As a natural compound, curcumin (Cur) exhibits excellent anti-inflammatory, anti-cancer properties few side effects. In this study, total of 60 male mice (6-week-olds, 15 per group). After one week acclimatization feeding, the were divided into control group (Con), AFB1 group, (Cur), AF+Cur group. The gavaged (Cur, 100 mg/kg) and/or (0.75 mg/kg). To identify new therapeutic...

This retrospective study was conducted to determine the prevalence and molecular epidemiology characteristics of carbapenem-resistant Escherichia coli (CRE).A total 593 (E. coli) isolates were recovered from pigs urban river 2009 2014 in Heilongjiang Province China. Forty CRE including 22 strains isolated fecal samples 18 water selected. PCR detection resistance determinants, multi-locus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), phylogenetic groups performed...

Atrial remodeling in the form of fibrosis is considered as substrate for development atrial fibrillation (AF). The aim this study was to investigate effects tolvaptan on chronic intermittent hypoxia (CIH) induced and mechanisms underlying such changes.A total 45 Sprague-Dawley rats were randomized into three groups: Control group, CIH with treatment (CIH-T) group (n = 15). subjected 6 hour/d 30 days, CIH-T administrated while they received CIH. After echocardiography examination, sacrificed...

Improper use of antimicrobials results in poor treatment and severe bacterial resistance. Breakpoints are routinely used the clinical laboratory setting to guide decision making. Therefore, objective this study was establish antimicrobial susceptibility breakpoints for danofloxacin against Escherichia coli (E.coli), which is an important pathogen digestive tract infections.The minimum inhibitory concentrations (MICs) 1233 E. isolates were determined by microdilution broth method accordance...

Abstract Improper use of antibiotics results in poor treatment and severe bacterial resistance. In this study, ultrafiltration probes were successfully placed the ileum piglets with aid anesthetic. After fluoroquinolone antimicrobial drug danofloxacin (DAN) was intramuscularly administered, blood ultrafiltrate collected at different time points then determined by High Performance Liquid Chromatography (HPLC). Pharmacokinetics (PK) parameters for plasma calculated WinNonlin software. The DAN...

Acute lung injury (ALI) is an inflammatory pulmonary disease that can easily develop into serious acute respiratory distress syndrome, which has high morbidity and mortality. However, the molecular mechanism of ALI remains unclear, few biomarkers for diagnosis treatment have been identified. In this study, we aimed to identify novel using a bioinformatics approach. Gene expression data were obtained from Expression Omnibus database, co-expressed differentially expressed genes (CoDEGs)...

Aflatoxin B1 (AFB1) is an unavoidable environmental toxin. The accumulation of AFB1 and its metabolites in the liver poses a threat to both human animal health. Curcumin exhibits anti-oxidative, anti-tumor anti-inflammatory properties. There no report on mechanism regarding how curcumin relived necroptosis chickens induced by based regulatory network ceRNA. To explore this, we performed transmission electron microscopy sequenced lncRNA mRNA chicken livers treated with and/or for 28 days...

Abstract Background Apramycin is used exclusively for the treatment of Escherichia coli ( E.coli ) infections in swine around world since early 1980s. Recently, many research papers have demonstrated that apramycin has significant vitro activity against multidrug-resistant isolated hospitals. Therefore, ensuring proper use veterinary clinics great significance public health. The objectives this study were to develop a wild-type cutoff using statistical method recommended by Clinical and...

To investigate the potential effects and mechanism on peroxisome proliferator-activated receptor γ-toll-like 4-tumor necrosis factor-α (PPARγ-TLR4-TNF-α) targeted pathway hyperglycemia induced myocardium inflammation oxidative stress.Thirty-two Japanese healthy adult rabbits were randomly divided into four groups with 8 in each group: normal control group (NC group), diabetes mellitus (DM + pioglitazone 4 mg×kg-1×d-1 (DM+PGZ mg groups). DM model was reproduced by alloxan of 150 mg/kg through...

Abstract Background： Apramycin is used exclusively for the treatment of Escherichia coli ( E.coli ) infections in swine around world since early 1980s. Recently, many research papers have demonstrated that apramycin has significant vitro activity against multidrug-resistant isolated hospitals. Therefore, ensuring proper use veterinary clinics great significance public health. The objectives this study were to develop a wild-type cutoff using statistical method recommended by Clinical and...

Abstract Background： Apramycin is used exclusively for the treatment of Escherichia coli ( E.coli ) infections in swine around world since early 1980s. Recently, many research papers have demonstrated that apramycin has significant vitro activity against multidrug-resistant isolated hospitals. Therefore, ensuring proper use veterinary clinics great significance public health. The objectives this study were to develop a wild-type cutoff using statistical method recommended by Clinical and...