A5101971244- Microfluidic and Bio-sensing Technologies
- Pluripotent Stem Cells Research
- 3D Printing in Biomedical Research
- Orbital Angular Momentum in Optics
- CRISPR and Genetic Engineering
- Neuroscience and Neural Engineering
- Renal and related cancers
- MicroRNA in disease regulation
- Hepatitis B Virus Studies
- CAR-T cell therapy research
- Chronic Lymphocytic Leukemia Research
- Wireless Body Area Networks
- Periodontal Regeneration and Treatments
- Advanced biosensing and bioanalysis techniques
- Cellular Mechanics and Interactions
- Cervical Cancer and HPV Research
- Mesenchymal stem cell research
- Microfluidic and Capillary Electrophoresis Applications
- Single-cell and spatial transcriptomics
- Hepatitis C virus research
- Liver Diseases and Immunity
- Multiple Myeloma Research and Treatments
- Planarian Biology and Electrostimulation
- dental development and anomalies
City University of Hong Kong
2015-2018
Guangzhou Institutes of Biomedicine and Health
2013-2016
Chinese Academy of Sciences
2013-2016
University of Science and Technology of China
2013
Stomatology Hospital
2007
Peking University
2007
Induced pluripotent stem cell (iPS cell) holds great potential for applications in regenerative medicine, drug discovery, and disease modeling. We describe here a practical method to generate human iPS cells from urine-derived (UCs) under feeder-free, virus-free, serum-free condition without oncogene c-MYC. showed that this approach could be applied large population with different genetic backgrounds. UCs are easily accessible exhibit high reprogramming efficiency, offering advantages over...
Single cell surgery such as manipulation or removal of subcellular components or/and organelles from single cells is increasingly used for the study diseases and their causes in precision medicine. This paper presents a robotic system to achieve automated organelle biopsy with dimensions less than 20 μm diameter. The complexity spatial detection position reduced by patterning using microfluidic chip device. A sliding mode nonlinear controller developed enable extraction organelles,...
Generation of induced pluripotent stem cells (iPSCs) from human urine-derived (hUCs) provides a convenient and non-invasive way to obtain patient-specific iPSCs. However, many isolated hUCs exhibit very poor proliferation are difficult reprogram. In this study, we optimized reprogramming approaches for with proliferation. We report here that compound cocktail containing cyclic pifithrin-a (a P53 inhibitor), A-83-01, CHIR99021, thiazovivin, NaB, PD0325901 significantly improves the efficiency...
Differentiation of neural lineages from human pluripotent stem cells (hPSCs) raises the hope generating functional for treatment diseases. However, current protocols differentiating hPSCs into remain inefficient and largely variable between different hPSC lines. We report that microRNA 376c (miR-376c) significantly enhanced differentiation in a defined condition by suppressing SMAD4, co-SMAD TGF-β signaling. Downstream, SMAD4 directly bound suppressed PAX6, critical lineage specification...
Estrogen has been shown to up-regulate the expression of osteoblastic phenotypes human periodontal ligament cells via binding estrogen receptors and may also help tissue regeneration. However, which subtype receptor (alpha or beta) is predominately expressed in cells, how regulated during osteogenic differentiation still unclear. This study aimed explore regulation subtypes their differentiation.Human derived from 10 individual age-matched donors (five male five female donors) were cultured....
Cell-to-cell interactions are complex processes that involve physical interactions, chemical binding, and biological signaling pathways. Identification of the functions special pathway in cell-to-cell interaction from very first contact will help characterize mechanism underlying advance new drug discovery. This paper reported a case study characterizing initial between leukemia cancer cells bone marrow stromal cells, through use an optical tweezers-based cell manipulation tool. Optical...
Single-cell culture represents the most straightforward method for investigating cellular heterogeneity. In this paper, we present a novel microfluidic platform that can individually array and hundreds of cells under chemical electrical stimuli week-scale characterization. Single be deterministically gently captured in microchamber on proposed platform. The size adjusted to fit different cell times, characteristic enables remarkable scalability. Transparent indium tin oxide microelectrodes...
One of the greatest challenges in acute myeloid leukemia (AML) treatment is preventing relapse. Leukemia cells can hide bone marrow niche or vascular niche. Hence, many chemical drugs cannot kill these cells. To characterize migration and adhesion properties specific niches, CXCR4/SDF-1α signal pathway has been widely used for investigation. AMD3100 treated as one most common that inhibit this signal. In current study, we particularly investigate effect on property stromal by using...
Mesenchymal stromal/stem cells (MSCs) are easily obtained multipotent that widely applied in regenerative medicine. Electrical stimulation (ES) has a promoting effect on bone healing and osteogenic differentiation of MSCs. Direct alternating currents (AC) extensively used to promote the MSCs vivo vitro. However, information conducting effective remains scarce. In this paper, we propose method optimize ES parameters based calcium spike patterns Calcium frequency decreases as MSC progresses....
This paper presents a microarray platform that allows high-throughput single cell capture in large microwells to support single-cell culture. work will facilitate the study of heterogeneity as well high efficient stimulation parameters (chemical and physical) screening. An U-shaped trap, combination two streamline focusing arms, is placed at bottom each microwell unit. The has 512 units. whole device fabricated by standard soft-lithography technology. ability this structure for...