A5087258668- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Renal and related cancers
- Microbial Metabolic Engineering and Bioproduction
- RNA Research and Splicing
- Chronic Kidney Disease and Diabetes
- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Single-cell and spatial transcriptomics
- Traumatic Brain Injury and Neurovascular Disturbances
- Renal Diseases and Glomerulopathies
- DNA Repair Mechanisms
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
- Dementia and Cognitive Impairment Research
- RNA Interference and Gene Delivery
- Cerebrovascular and Carotid Artery Diseases
- Pancreatic function and diabetes
- Plant biochemistry and biosynthesis
- Algal biology and biofuel production
- Gene expression and cancer classification
- Anesthesia and Neurotoxicity Research
- Neurological Disease Mechanisms and Treatments
- Microbial Metabolites in Food Biotechnology
- Cancer-related molecular mechanisms research
Soochow University
2020-2025
Northeast Agricultural University
2024-2025
Shaoguan University
2025
Guangzhou Institutes of Biomedicine and Health
2017-2024
Chinese Academy of Sciences
2016-2024
Microbiology Institute of Shaanxi
2024
Institute of Microbiology
2024
Duke-NUS Medical School
2017-2024
Hebei Normal University of Science and Technology
2024
Shanghai University of Engineering Science
2024
Our understanding of human early development is severely hampered by limited access to embryonic tissues. Due their close evolutionary relationship with humans, nonhuman primates are often used as surrogates understand but currently suffer from a lack in vivo datasets, especially gastrulation organogenesis during which the major cell types dynamically specified. To fill this gap, we collected six Carnegie stage 8-11 cynomolgus monkey (Macaca fascicularis) embryos and performed in-depth...
Spatially resolved transcriptomics provides the opportunity to investigate gene expression profiles and spatial context of cells in naive state, but at low transcript detection sensitivity or with limited throughput. Comprehensive annotating cell types spatially understand biological processes single level remains challenging. Here we propose Spatial-ID, a supervision-based typing method, that combines existing knowledge reference single-cell RNA-seq data information data. We present series...
Development of physiologically relevant cellular models with strong translatability to human pathophysiology is critical for identification and validation novel therapeutic targets. Herein we describe a detailed protocol generation an advanced 3-dimensional kidney model using induced pluripotent stem cells, where differentiation maturation progenitors podocytes can be monitored in live cells due CRISPR/Cas9-mediated fluorescent tagging lineage markers (SIX2 NPHS1). Utilizing these cell...
Highlights•Jdp2, Jhdm1b, Mkk6, Glis1, Nanog, Esrrb, and Sall4 (7F) convert MEFs into iPSCs•RNA-seq ATAC-seq reveal a distinct path for 7F reprogramming•7F cooperate to open close chromatin during activate TF network induce pluripotencySummaryReprogramming somatic cells pluripotency by Oct4, Sox2, Klf4, Myc represent paradigm cell fate determination. Here, we report combination of Jdp2, Essrb, that reprogram mouse embryonic fibroblasts or chimera competent induced pluripotent stem (iPSCs)...
Background: Ovarian cancer (OC) is one of the most malignant tumors in female reproductive system, with a poor prognosis. Various responses to treatments including chemotherapy and immunotherapy are observed among patients due their individual characteristics. Applicable prognostic markers could make it easier refine risk stratification for OC patients. Autophagy closely implicated occurrence development tumors, OC. Whether autophagy -related genes can be used as remains unclear. Methods:...
Cell fate decision involves rewiring of the genome, but remains poorly understood at chromatin level. Here, we report that remodeling complex NuRD participates in closing open early phase somatic reprogramming. Sall4, Jdp2, Glis1 and Esrrb can reprogram MEFs to iPSCs efficiently, only Sall4 is indispensable capable recruiting endogenous components NuRD. Yet knocking down reduces reprogramming modestly, contrast disrupting known Sall4-NuRD interaction by mutating or deleting interacting motif...
Key Points WWP2 expression is elevated in the tubulointerstitium of fibrotic kidneys and contributes to CKD pathogenesis progression. uncouples profibrotic activation cell proliferation renal myofibroblasts. controls mitochondrial respiration myofibroblasts through metabolic regulator peroxisome proliferator-activated receptor gamma coactivator 1-alpha. Background Renal fibrosis a common pathologic end point that challenging reverse, are responsible for accumulation fibrillar collagen–rich...
Abstract Background MUC16 (CA125) is a commonly used tumor marker for ovarian cancer screening and reported to be an immunosuppressive factor by acting on the sialic acid-binding immunoglobulin-like lectin-9 (Siglec-9) surface of natural killer cells (NK cells), B cells, monocytes. However, role neutrophils in microenvironment remains further explored. Methods The correlation between proportion count peripheral blood serum inflammatory-related factors level patients was constructed based...
Type I CRISPR-Cas systems are widespread and have exhibited high versatility efficiency in genome editing gene regulation prokaryotes. However, due to the multi-subunit composition large size, their application eukaryotes has not been thoroughly investigated. Here, we demonstrate that type I-F2 Cascade, most compact among systems, with a total size smaller than of SpCas9, can be developed for transcriptional activation human cells. The engineered tool match or surpass dCas9. Additionally,...
This study evaluated the reliability and validity of In-Out-Test for detecting episodic memory deficits in stroke patients explored its potential as a clinical test. A total 75 120 healthy controls underwent tests, including Mini-Mental State Examination (MMSE), Montreal Cognitive Assessment (MoCA), Picture-Based Memory Impairment Screen (PMIS), In-Out-Test. Reliability metrics (Cronbach's α, inter-scorer reliability, test-retest reliability), criterion validity, corrected item-total...
The mitochondrial succinate dehydrogenase (SDH) is a tetrameric iron-sulfur flavoprotein of the Krebs cycle and respiratory chain. A number mutations in human SDH genes are responsible for development paragangliomas, cancers head neck region. mev-1 mutation Caenorhabditis elegans gene encoding homolog SDHC subunit results premature aging hypersensitivity to oxidative stress. It also increases production superoxide radicals by enzyme. In this work, we used yeast investigate molecular...
CRISPR/dCas9 is a versatile tool that can be used to recruit various effectors and fluorescent molecules defined genome regions where it modulate genetic epigenetic markers, or track the chromatin dynamics in live cells. In vivo applications of animals have been challenged by delivery issues. We generate characterize mouse strain with dCas9-EGFP ubiquitously expressed tissues. Studying telomere these reveals surprising results different from those observed cultured cell lines. The knock-in...
Abstract Class II HDACs, such as HDAC4, are critical regulators of the immune response in various cells; however, its role innate immunity remains largely unknown. Here, we report that overexpression HDAC4 suppresses production type I interferons triggered by pattern-recognition receptors (PRRs). repressed translocation transcription factor IRF3 to nucleus, thereby decreasing IRF3-mediated IFN-β expression. In particular, also determined can be phosphorylated and simultaneously block...
The purpose of this study was to investigate the influence education level on MMSE and MoCA scores elderly inpatients.This is a cross-sectional study. A total 260 inpatients were evaluated by sequentially.The highly correlated with (r = 0.7629, p < 0.0001), length 0.2723, 0.0001). also 0.3323, Meanwhile, used reevaluate normal at different levels. There no significant differences levels (χ2=1.351, 0.5090). Finally, we analyzed diagnostic consistency levels, results showed that closely...
Abstract Background Owing to recent advances in resolution and field-of-view, spatially resolved transcriptomics sequencing, such as Stereo-seq, has emerged a cutting-edge technology for the interpretation of large tissues at single-cell level. To generate accurate spatial gene expression profiles from high-resolution omics data, powerful computational tool is required. Findings We present CellBin, an image-facilitated one-stop pipeline field-of-view transcriptomic data Stereo-seq. CellBin...
The fuel ethanol industry has made tremendous progress in the last decades. Ethanol can be obtained by fermentation using a variety of biomass materials as feedstocks. However, few studies have been conducted on utilization microorganisms. price petroleum-derived ethanol, easily hydrolysis ethylene, is even lower than that bioethanol. If metabolized microorganisms to produce value-added chemicals, it will open new door for inexpensive resources. We constructed an engineered Escherichia coli...