A5103116537- Reproductive System and Pregnancy
- Renal and related cancers
- Pregnancy and preeclampsia studies
- Single-cell and spatial transcriptomics
- Pluripotent Stem Cells Research
- Magnetic Properties of Alloys
- COVID-19 Impact on Reproduction
- Metallic Glasses and Amorphous Alloys
- HIV Research and Treatment
- Mesenchymal stem cell research
- Reproductive Biology and Fertility
- Shape Memory Alloy Transformations
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Astronomy and Astrophysical Research
- Prenatal Screening and Diagnostics
- Animal Genetics and Reproduction
- Pharmacology and Obesity Treatment
- Animal Disease Management and Epidemiology
- Heusler alloys: electronic and magnetic properties
- Immune Cell Function and Interaction
- RNA Research and Splicing
- Adaptive optics and wavefront sensing
- Tissue Engineering and Regenerative Medicine
- Genetics, Aging, and Longevity in Model Organisms
Hebei University of Technology
2023-2024
Anhui Provincial Hospital
2024
Anhui Medical University
2022-2024
Ministry of Education of the People's Republic of China
2024
First Affiliated Hospital of Anhui Medical University
2023-2024
Beijing Forestry University
2024
Research Institute of Wood Industry
2024
Chinese Academy of Forestry
2024
Institute of Zoology
2018-2023
Chinese Academy of Sciences
2008-2023
In vitro development of monkey embryos Owing to technical and ethical limitations, the molecular cellular mechanisms underlying primate gastrulation are far from clear (see Perspective by Tam). Two independent studies used an in culture system study cynomolgus embryo postimplantation up beyond (day 9 day 20). Niu et al. observed vivo morphogenetic events single-cell RNA sequencing chromatin accessibility distinct cell lineages developing embryos. Ma also that key early were recapitulated...
Mesenchymal stem cells (MSCs) have emerged as a promising regenerative tool, owing mainly to their multi-differentiation potential and immunosuppressive capacity. When compared with MSCs classically derived from the adult bone marrow (BM), of neonatal origins exhibit superior proliferation ability, lower immunogenicity, possible incorporated mutation; hence, they are considered an alternative source for clinical use. Several researches focused on biological differences among some cultured in...
Abstract The pluripotency of mammalian early and late epiblast could be recapitulated by naïve embryonic stem cells (ESCs) primed (EpiSCs), respectively. However, these two states may not sufficient to reflect the full complexity developmental potency during development. Here we report establishment self-renewing formative pluripotent (fPSCs) which manifest features poised for gastrulation. fPSCs can established from different mouse ESCs, pre-/early-gastrula epiblasts induced PSCs. Similar...
Our understanding of human early development is severely hampered by limited access to embryonic tissues. Due their close evolutionary relationship with humans, nonhuman primates are often used as surrogates understand but currently suffer from a lack in vivo datasets, especially gastrulation organogenesis during which the major cell types dynamically specified. To fill this gap, we collected six Carnegie stage 8-11 cynomolgus monkey (Macaca fascicularis) embryos and performed in-depth...
The placenta is one of the most important yet least understood organs. Due to limitations conventional research approaches, we are still far from a comprehensive understanding mouse placentation, especially regarding differentiation trophoblast lineages at early developmental stage. To decipher cell compositions and processes, systematically profile single-cell transcriptomes cells extraembryonic tissues (embryonic day 7.5 (E7.5) E8.5) placentae (E9.5-E14.5) one-day intervals. We identify...
ABSTRACT Proper differentiation of trophoblast cells in the human placenta is a prerequisite for successful pregnancy, and dysregulation this process may lead to malignant pregnancy outcomes, such as preeclampsia. Finding specific markers different types essential understanding differentiation. Here, we report that placenta-specific protein 8 (PLAC8) specifically expressed interstitial extravillous (iEVTs) on fetomaternal interface. Using model systems, including placental villi-decidua...
To examine the characteristics and distribution of possible severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) target cells in human trophectoderm (TE) placenta.Bioinformatics analysis was performed based on published single-cell transcriptomic datasets early TE first- second-trimester placentae. We conducted 4198 cells, 1260 first-trimester placental 189 extravillous trophoblast (EVTs) from 24-week placentae (EVT_24W) using SMART-Seq2 method. In addition, to confirm bioinformatic...
Gonad somatic cells acquire sex-specific fates during sex determination. In XX gonad, a subset of expresses Foxl2 after determination which is considered the progenitor granulosa cells. However, whether these also contribute to other cell types at later developmental stages unknown. present study, fate Foxl2-expressing in fetal ovaries was analyzed by lineage tracing and single-cell transcriptomics. We found that gave rise three stages, including cells, theca-interstitial stromal Series RNA...
C-C chemokine receptor type 5 (CCR5) is the main co-receptor for HIV entry into target CD4+ cells, and homozygous CCR5Δ32/Δ32 cells are resistant to CCR5-tropic infection. However, donors in populations rare. Here we developed a simple approach induce homozygotes through CRISPR-Cas9 genome-editing technology. Designing pair of single-guide RNA targeting flank region CCR5Δ32 mutation locus, applied lentiviral packaging system successfully convert wild-type CCR5 human Jurkat cell line primary...
Abstract Objectives NCOA6 is a transcription coactivator; its deletion in mice results growth retardation and lethality between 8.5 12.5 dpc with defects the placenta. However, factor(s) mechanism(s) involved function of placentation have not been elucidated. Here, roles human cytotrophoblast invasion migration were studied. Materials Methods Human placenta tissues collected from normal pregnancies complicated by early‐onset severe preeclampsia (sPE). Immunofluorescence, RT‐qPCR Western...
Upon implantation, the trophectoderm differentiates into multi-nucleated primitive syncytiotrophoblast (pSTB) through a process called primary syncytialization to facilitate maternal-fetal interactions and establish pregnancy. However, ethical issues limited access human embryos around time of embryo implantation hinder investigation detailed molecular mechanisms underpinning this event in humans. Here we established trophoblast stem cells (hTSCs) from blastocysts. We characterized nuclear...
Abstract Normally, HIV-1 enters into CD4+ cells through membrane fusion, and newly synthesized viral proteins assemble on the plasma to form particles bud out. In previous study, we found host factor coiled-coil domain containing protein 8 (CCDC8) can strongly inhibit production, but underline mechanism is not clear. Here show that overexpression of CCDC8 reverses normal production process, causes assembled Gag be endocytosed membrane, rather than budding Live-cell imaging system captured...
Implantation of the human blastocyst is a milestone event in embryonic development. The trophoblast first cell lineage to differentiate during implantation. Failures differentiation implantation are correlated defects pregnancy and growth. However, many gaps remain knowledge development, especially regarding morphogenesis function. Herein, we performed single-cell RNA sequencing (scRNA-seq) analysis on post-implantation embryos cultured vitro. A hierarchical model was established, which...
Single-phase Mn50Ni40In10−xCux (x = 0, 1, 2, 4, 6, 8, and 10) Heusler alloys were synthesized by melt-spinning method. Martensitic transformation is retained within the whole composition range studied. The substitution of Cu for In leads to a drastic increase in martensitic temperature TM from below room above 750 K, enhances antiferromagnetic character martensite. related increasing electron concentration e/a decreasing cell volume V with doping. energy difference between martensite...
Abstract The placenta, which originates from the trophectoderm (TE), is first organ to form during mammalian embryogenesis. Recent studies based on bioinformatics analysis have revealed that heterogeneous gene expression initiates cell-fate decisions and directs two distinct cell fates by modulating balance of pluripotency differentiation as early four-cell stage. However, direct developmental evidence support this still lacking. To address at stage fate TE inner mass (ICM) determined, in...
African swine fever (ASF) is a contagious viral disease that affects domestic pigs and wild boars, causing significant economic losses globally. After the first Nigerian outbreak in 1997, there have been frequent reports of ASF pig-producing regions country. To facilitate control, it important to understand genotype phylogenetic relationship viruses (ASFVs). Recent genetic analysis ASFV isolates has revealed presence both genotypes I II; this based on few selected genes. Phylogenetic whole...