A5077970037- Pluripotent Stem Cells Research
- RNA modifications and cancer
- RNA Research and Splicing
- Reproductive Biology and Fertility
- Renal and related cancers
- Liver physiology and pathology
- Single-cell and spatial transcriptomics
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Sperm and Testicular Function
- Cancer-related gene regulation
Chinese Academy of Sciences
2015-2024
Kunming Institute of Zoology
2019-2024
Genetic Analysis (Norway)
2024
Institute of Zoology
2015-2019
University of Chinese Academy of Sciences
2015
In vitro development of monkey embryos Owing to technical and ethical limitations, the molecular cellular mechanisms underlying primate gastrulation are far from clear (see Perspective by Tam). Two independent studies used an in culture system study cynomolgus embryo postimplantation up beyond (day 9 day 20). Niu et al. observed vivo morphogenetic events single-cell RNA sequencing chromatin accessibility distinct cell lineages developing embryos. Ma also that key early were recapitulated...
Breast cancer amplified sequence 2 (BCAS2) is involved in multiple biological processes, including pre-mRNA splicing. However, the physiological roles of BCAS2 are still largely unclear. Here we report that specifically enriched spermatogonia mouse testes. Conditional disruption Bcas2 male germ cells impairs spermatogenesis and leads to infertility. Although appear grossly normal, spermatocytes meiosis prophase I events (recombination synapsis) rarely observed BCAS2-depleted testis. In null...
Eras encodes a Ras-like GTPase protein that was originally identified as an embryonic stem cell-specific Ras. ERAS has been known to be required for the growth of cells and stimulates somatic cell reprogramming, suggesting its roles on mouse early development. We now report dynamic expression pattern during peri-implantation development: increases at blastocyst stage, specifically decreases in E7.5 mesoderm. In accordance with pattern, increased promotes proliferation through controlling AKT...
Maintaining genomic stability is crucial for embryonic stem cells (ESCs). ESCs with unrepaired DNA damage are eliminated through differentiation and apoptosis. To date, only tumor suppressor p53 known to be implicated in this quality control process. Here, we identified a p53-independent factor lncRNA NONMMUT028956 (Lnc956 short) mouse ESCs. Lnc956 prevalently expressed regulates the of after damage. Mechanistically, Ataxia telangiectasia mutated (ATM) activation drives m6A methylation...
Abstract Spermatogonial stem cells (SSCs) play crucial roles in the preservation of male fertility. However, successful ex vivo expansion authentic human SSCs remains elusive due to inadequate understanding SSC homeostasis regulation. Using rhesus monkeys (Macaca mulatta) as a representative model, we characterized and progenitor subsets through single-cell RNA sequencing using cell-specific network approach. We also profiled chromatin status major histone modifications (H3K4me1, H3K4me3,...