A5100357154- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- MicroRNA in disease regulation
- Pancreatic and Hepatic Oncology Research
- Cancer, Hypoxia, and Metabolism
- Circular RNAs in diseases
- Photodynamic Therapy Research Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Colorectal Cancer Treatments and Studies
- Nonmelanoma Skin Cancer Studies
- Ferroptosis and cancer prognosis
- Bioinformatics and Genomic Networks
- Cancer Genomics and Diagnostics
- Dark Matter and Cosmic Phenomena
- Autophagy in Disease and Therapy
- Gene expression and cancer classification
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Genetic factors in colorectal cancer
- Cancer Research and Treatments
- Cytokine Signaling Pathways and Interactions
- Meningioma and schwannoma management
- Genomics and Phylogenetic Studies
- Particle physics theoretical and experimental studies
Sun Yat-sen University
2015-2025
Longgang Central Hospital
2025
Hangzhou City University
2024-2025
Harbin Medical University
2012-2024
Northwest Normal University
2024
Tianjin Medical University Cancer Institute and Hospital
2016-2024
Shanxi Medical University
2012-2024
Tsinghua University
2013-2024
Zhejiang Sci-Tech University
2024
Peking University
2009-2024
The long-term integrity of an articulating joint is dependent upon the nourishment its cartilage component and protection surface from friction-induced wear. Loss-of-function mutations in lubricin (a secreted glycoprotein encoded by gene PRG4) cause human autosomal recessive disorder camptodactyly-arthropathy-coxa vara-pericarditis syndrome (CACP). A major feature CACP precocious failure. In order to delineate mechanism which protects joints, we studied expression Prg4 mRNA during mouse...
Patients with newly diagnosed multiple myeloma (NDMM) high-risk disease are in need of new treatment strategies to improve the outcomes. Multiple clinical, cytogenetic, or gene expression features have been used identify patients, each which has significant weaknesses. Inclusion molecular into risk stratification could resolve current challenges. In a genome-wide analysis largest set and clinical data established date from NDMM, as part Myeloma Genome Project, we defined DNA drivers...
Evidence for transcriptional activation of the viral oncoproteins E6 and E7 is regarded as gold standard presence clinically relevant human papillomavirus (HPV), but detection E6/E7 mRNA requires RNA extraction polymerase chain reaction amplification-a challenging technique that restricted to research laboratory. The development in situ hybridization (ISH) probes complementary permits direct visualization transcripts routinely processed tissues has opened door accurate HPV clinical care...
Summary Impaired local cellular immunity contributes to the pathogenesis of persistent high‐risk human papillomavirus ( HR ‐ HPV ) infection and related cervical intraepithelial neoplasia CIN ), but underlying molecular mechanisms remain unclear. Recently, programmed death 1/programmed 1 ligand PD ‐1/ L 1; CD 279/ 274) pathway was demonstrated play a critical role in attenuating T ‐cell responses promoting tolerance during chronic viral infections. In this study, we examined expression ‐1 on...
Although Rho-associated kinase (ROCK) activity has been implicated in cardiovascular diseases, the tissue- and isoform-specific roles of ROCKs vascular response to injury are not known. To address role this process, we generated haploinsufficient Rock1 (Rock1(+/-)) Rock2 (Rock2(+/-)) mice performed carotid artery ligations. Following intervention, found reduced neointima formation Rock1(+/-) compared with that WT or Rock2(+/-) mice. This correlated decreased smooth muscle cell proliferation...
Abstract Motivation: The differential expression analysis focusing on inter-group comparison can capture only differentially expressed genes (DE genes) at the population level, which may mask heterogeneity of in individuals. Thus, to provide patient-specific information for personalized medicine, it is necessary conduct individual level. Results: We proposed a method detect DE disease samples by using disrupted ordering samples. In both simulated data and real paired cancer-normal sample...
To improve chemical cross-linking of proteins coupled with mass spectrometry (CXMS), we developed a lysine-targeted enrichable cross-linker containing biotin tag for affinity purification, cleavage site to separate cross-linked peptides away from after enrichment, and spacer arm that can be labeled stable isotopes quantitation. By locating the flexible on surface 70S ribosome, show this trifunctional is effective at attaining structural information not easily attainable by crystallography...
PtdIns3P signaling is critical for dynamic membrane remodeling during autophagosome formation. Proteins in the Atg18/WIPI family are PtdIns3P-binding effectors which can form complexes with proteins Atg2 family, and both families essential macroautophagy/autophagy. However, little known about biophysical properties biological functions of Atg2-Atg18/WIPI complex as a whole. Here, we demonstrate that an ortholog yeast Atg18, mammalian WDR45/WIPI4 has stronger binding capacity ATG2A or ATG2B...
Most of current gene expression signatures for cancer prognosis are based on risk scores, usually calculated as some summaries levels the signature genes, whose applications require presetting score thresholds and data normalization. In this study, we demonstrate critical limitations such type that scores samples will change greatly when they normalized together with different samples, which would induce spurious classification difficulty in clinical settings, independent incomparable if...
Background and Purpose: The benefit of endovascular treatment (EVT) for large vessel occlusion in clinical practice developing countries like China needs to be confirmed. aim the study was determine whether EVT acute ischemic stroke randomized trials could generalized Chinese population. Methods: We conducted a prospective registry at 111 centers China. Patients with caused by imaging-confirmed intracranial receiving were included. primary outcome functional independence 90 days defined as...
Abstract riboCIRC is a translatome data-oriented circRNA database specifically designed for hosting, exploring, analyzing, and visualizing translatable circRNAs from multi-species. The provides comprehensive repository of computationally predicted ribosome-associated circRNAs; manually curated collection experimentally verified translated an evaluation cross-species conservation systematic de novo annotation putative circRNA-encoded peptides, including sequence, structure, function; genome...
Background and Purpose: A recent randomized controlled trial DIRECT-MT (Direct Intra-Arterial Thrombectomy to Revascularize AIS Patients With Large Vessel Occlusion Efficiently in Chinese Tertiary Hospitals) compared the safety efficacy of mechanical thrombectomy (MT) versus combined intravenous thrombolysis (IVT) MT for acute large vessel occlusion. The current study utilized a prospective, nationwide registry validate results real-world practice setting. Methods: Subjects were selected...
Abstract Human ribosomes have long been thought to be uniform factories with little regulatory function. Accumulating evidence emphasizes the heterogeneity of ribosomal protein (RP) expression in specific cellular functions and development. However, a systematic understanding functional relevance RPs is lacking. Here, we surveyed translational transcriptional changes after individual knockdown 75 RPs, 44 from large subunit (60S) 31 small (40S), by Ribo-seq RNA-seq analyses. Deficiency...
The epigenomic abnormality of pancreatic ductal adenocarcinoma (PDAC) has rarely been investigated due to its strong heterogeneity. Here, we used single-cell multiomics sequencing simultaneously analyze the DNA methylome, chromatin accessibility and transcriptome in individual tumor cells PDAC patients. We identified normal epithelial lesion, which have euploid genomes, patterns methylation, accessibility. Using all these as controls, determined that demethylation cancer genome was strongly...
Abstract Molecular variation between geographical populations and subtypes indicate potential genomic heterogeneity novel features within CCA. Here, we analyze exome-sequencing data of 87 perihilar cholangiocarcinoma (pCCA) 261 intrahepatic (iCCA) cases from 3 Asian centers (including 43 pCCAs 24 iCCAs our center). iCCA tumours demonstrate a higher tumor mutation burden copy number alteration (CNAB) than pCCA tumours, high CNAB indicates poorer prognosis. We identify 12 significantly mutated...
Abstract As a common pathology of many ocular disorders such as diabetic retinopathy and glaucoma, retinal ischemia/reperfusion (IR) triggers inflammation microglia activation that lead to irreversible damage. The detailed molecular mechanism underlying IR injury, however, remains poorly understood at present. Here we report the bioinformatic identification lncRNA 1810058I24Rik ( 181-Rik ) was shown encode mitochondrion-located micropeptide Stmp1. Its deficiency in mice protected ganglion...
Abstract VEGF inhibitors are one of the most successful antiangiogenic drugs in treatment many solid tumors. Nevertheless, pancreatic adenocarcinoma (PAAD) cells can reinstate tumor angiogenesis via activation VEGF-independent pathways, thereby conferring resistance to inhibitors. Bioinformatic analysis showed that BICC1 was top genes involved specific process PAAD. The our own cohort confirmed overexpressed human PAAD tissues and correlated increased microvessel density growth, worse...
Abstract Plasmacytoid dendritic cells (pDC), as major producers of IFN-α, are thought not only to be pivotal in antiviral immunity, but also limit allergic inflammation. In this study, we delineate the role pDC a mouse model respiratory syncytial virus (RSV)-induced airway Bone marrow-derived generated high levels IFN-α upon RSV infection, and percentage expressing MHC class II maturation-associated costimulatory molecules was increased. However, their weak Ag-presenting capacity enhanced....
Head and neck squamous cell carcinoma (HNSCC) is characterized by significant genomic instability that could lead to clonal diversity. Intratumor heterogeneity has been proposed as a major attribute underlying tumor evolution, progression, resistance chemotherapy radiation. Understanding genetic treatments specific resistant metastatic cells. To characterize the degree of intratumor within single tumor, we performed whole-genome sequencing on three separate regions an human papillomavirus...
Many bacteria contain an ortholog of the Ro autoantigen, a ring-shaped protein that binds noncoding RNAs (ncRNAs) called Y RNAs. In only studied bacterium, Deinococcus radiodurans, Rsr functions in heat-stress-induced ribosomal RNA (rRNA) maturation and starvation-induced rRNA decay. However, mechanism by which this conserved its associated ncRNAs act has been obscure. We report exoribonuclease polynucleotide phosphorylase (PNPase) form degradation machine is scaffolded RNA. Single-particle...