A5101027876- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Innovative Energy Harvesting Technologies
- Machine Learning in Bioinformatics
- Energy Harvesting in Wireless Networks
- Vibration Control and Rheological Fluids
- Fault Detection and Control Systems
- Biotin and Related Studies
- Protein Structure and Dynamics
- Advanced Sensor and Energy Harvesting Materials
- Metabolomics and Mass Spectrometry Studies
- Bioinformatics and Genomic Networks
- Magnetic Bearings and Levitation Dynamics
- Advanced machining processes and optimization
- Manufacturing Process and Optimization
- Machine Fault Diagnosis Techniques
- Wireless Power Transfer Systems
- Machine Learning and Data Classification
- Fluid Dynamics and Vibration Analysis
- Genomics and Phylogenetic Studies
- stochastic dynamics and bifurcation
- Enzyme Structure and Function
- Distributed and Parallel Computing Systems
- Advanced Measurement and Metrology Techniques
- Advanced Numerical Analysis Techniques
Tianjin University
2005-2025
Chinese Academy of Sciences
2012-2019
Institute of Computing Technology
2014-2019
University of Chinese Academy of Sciences
2014-2015
Chengdu University of Technology
2009
Abstract We describe pLink 2, a search engine with higher speed and reliability for proteome-scale identification of cross-linked peptides. With two-stage open strategy facilitated by fragment indexing, 2 is ~40 times faster than 1 3~10 Kojak. Furthermore, using simulated datasets, synthetic 15 N metabolically labeled entrapment databases, four analysis methods were designed to evaluate the credibility ten state-of-the-art engines. This systematic evaluation shows that outperforms these in...
To improve chemical cross-linking of proteins coupled with mass spectrometry (CXMS), we developed a lysine-targeted enrichable cross-linker containing biotin tag for affinity purification, cleavage site to separate cross-linked peptides away from after enrichment, and spacer arm that can be labeled stable isotopes quantitation. By locating the flexible on surface 70S ribosome, show this trifunctional is effective at attaining structural information not easily attainable by crystallography...
In relative protein abundance determination from peptide intensities recorded in full mass scans, a major complication that affects quantitation accuracy is signal interference coeluting ions of similar m/z values. Here, we present pQuant, software tool solves this problem. pQuant detects signals, identifies for each pair least interfered isotopic chromatograms: one the light and heavy isotope-labeled peptide. On basis these pairs, calculates heavy/light ratios along with their 99.75%...
Chemical cross-linking of proteins coupled with mass spectrometry (CXMS) is a powerful tool to study protein folding and map the interfaces between interacting proteins. The most commonly used cross-linkers in CXMS are BS(3) DSS, which have similar structures generate same linkages pairs lysine residues spatial proximity. However, there cases where no cross-linkable present at certain regions or interface two In order find that can best complement performance we tested seven additional...
Abstract pLink is a search engine for high‐throughput identification of cross‐linked peptides from their tandem mass spectra, which the data‐analysis step in chemical cross‐linking proteins coupled with spectrometry analysis. has accumulated more than 200 registered users all over world since its first release 2012. After 2 years continual development, new version been released, at least 40 times faster, versatile, and user‐friendly. Also, function expanded to identifying endogenous protein...
Mechanical fault diagnosis usually requires not only identification of the characteristic frequency, but also detection its second and/or higher harmonics. However, it is difficult to detect a multi-frequency signal through existing Stochastic Resonance (SR) methods, because frequency as well and harmonics frequencies tend be large parameters. To solve problem, this paper proposes method based on Frequency Exchange Re-scaling (FERSR). In method, exchange implemented using filtering technique...
A novel Nonlinear Eddy Current Damping (NL-ECD) design method for a Triple-magnet Magnetic Suspension Dynamic Vibration Absorber (TMSDVA) is proposed, modelled, and tested. The implementation of the NL-ECD completely passive does not require an external energy supply. First, mathematical model TMSDVA vibration reduction system established. Additionally, calculation also derived. coefficient can be designed adjusted by changing dimensional parameters damping tube. Then correctness derivation...
Disulfide bonds are vital for protein functions, but locating the linkage sites has been a challenge in chemistry, especially when quantity of sample is small or complexity high. In 2015, our laboratory developed sensitive and efficient method mapping disulfide from simple complex samples (Lu et al. Nat Methods 12:329, 2015). This based on liquid chromatography-mass spectrometry (LC-MS) powerful data analysis software tool named pLink. To facilitate application this method, we present...
Abstract This paper presents a mechanical-free method for providing and tailoring the nonlinear force in bistable piezoelectric energy harvesters (BPEHs). The can be tailored to obtain lower threshold inter-well motions, or harvester operate at various excitation levels frequencies without changing mechanical structure overall assembly. In BPEHs, is match specific level frequency, designed achieve higher strain (and thus output power). design of nonlinearity separated from by using magnetic...
Focused on the problems that development of grid application is difficult and type discovered resources restricted when directly using globus toolkit API, some technologies such as container, plug-in, object pool, resource adapter subgrid communication protocol for computing are introduced, kernel methods hiberarchy distributed middleware (CGDM) discussed in this paper. By applying these technologies, realized, which independent portal application. The real a campus shows can make it easy to...
A novel Triple-magnet Magnetic Suspension Dynamic Vibration Absorber is designed and modeled in this paper. The equivalent dynamic model of the vibration reduction system established. Meanwhile, calculation nonlinear magnetic suspension force derived. Based on model, it found that stiffness feature can be adjusted by changing magnet distance Absorber. Then equations are solved using Complex Variable Average method. And correctness derivation procedure verified through Runge–Kutta From...
Weak fault detection is crucial to incipient mechanical diagnosis. In order extract weak signals, a method named improved Re-scaling Frequency Stochastic Resonance (IRFSR) proposed in this paper. The consists of four steps: (i) Information Exchange (FIE); (ii) Amplitude Coefficient Adjustment; (iii) (RFSR); and (iv) Recovery. By means the exchange frequency information, high-frequency information target signal accordingly transferred low band which can be rescaled satisfy small-parameter...
In the study of nonlinear bi-stable piezoelectric cantilever energy harvesting system, accuracy magnetic force’s calculation on which potential function and dynamics system depend is essential to predict output response effect. this paper, we built a shape calculate trace end beam with integral entire beam’s slope, force consequently derived from achieved magnets’ real-time position posture using magnetizing currents method. With comprehensive consideration axial lateral force, change both...
With the rapid development of high throughput DNA sequencing,genomes more and species have been sequenced.Identifying determining structures coding genes are basic tasks genome annotation.To understand sequenced precisely,it is necessary to integrate muilti-omics data annotate genomes.However,the annotation methods developed in past decade mainly based on transcriptome data.Recently,mass spectrometry proteomics has come age,which can cover proteomes nearly completely make it possible use...