A5053152816- Endoplasmic Reticulum Stress and Disease
- Heme Oxygenase-1 and Carbon Monoxide
- Microtubule and mitosis dynamics
- Cellular transport and secretion
- Porphyrin Metabolism and Disorders
- CRISPR and Genetic Engineering
- Tryptophan and brain disorders
- DNA Repair Mechanisms
- Cancer, Hypoxia, and Metabolism
- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Treatment of Major Depression
- Advanced biosensing and bioanalysis techniques
- Immune Response and Inflammation
- Mental Health Research Topics
- Cancer-related Molecular Pathways
- Cancer Treatment and Pharmacology
- Enzyme Structure and Function
- Microbial Natural Products and Biosynthesis
- Phytochemistry and Bioactivity Studies
- Ubiquitin and proteasome pathways
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Neurological diseases and metabolism
- Biotin and Related Studies
Shanghai Jiao Tong University
2019-2025
Hunan University
2024
Columbia University
2014-2021
Ministry of Agriculture and Rural Affairs
2019
Guangdong Academy of Agricultural Sciences
2019
National Institute of Biological Sciences, Beijing
2011-2015
China Agricultural University
2012
Methodist Hospital
2012
Houston Methodist
2012
Capital Normal University
2012
Methylation of the N6 position selected internal adenines (m(6)A) in mRNAs and noncoding RNAs is widespread eukaryotes, YTH domain a collection proteins recognizes this modification. We report crystal structure splicing factor YT521-B homology (YTH) Zygosaccharomyces rouxii MRB1 complex with heptaribonucleotide an m(6)A residue center. The modification recognized by aromatic cage, being sandwiched between Trp Tyr methyl group pointed toward another residue. Mutations residues RNA binding...
Significance The small nucleotide cyclic di-3′,5′-adenosine monophosphate (c-di-AMP) recently emerged as a ubiquitous signaling molecule among bacteria, with essential roles in both bacterial physiology and host–pathogen interactions. Bacterial mutants abnormal c-di-AMP levels exhibit growth virulence defects, reflecting the importance of regulating synthesis degradation for normal signal transduction adaptation to changing environments. Previously documented phosphodiesterases hydrolyze via...
Technological advances in rare DNA mutations detection have revolutionized the diagnosis and monitoring of tumors, but they are still limited by lack supersensitive high-coverage procedures for identifying low-abundance mutations. Here, we describe a single-tube, multiplex PCR-based system, A-Star, that involves hyperthermophilic Argonaute from Pyrococcus furiosus (PfAgo) highly efficient beneficial its compatibility with polymerase. This novel technique uses specific guide design strategy...
The facultative intracellular pathogen Listeria monocytogenes, like many related Firmicutes, uses the nucleotide second messenger cyclic di-AMP (c-di-AMP) to adapt changes in nutrient availability, osmotic stress, and presence of cell wall-acting antibiotics. In rich medium, c-di-AMP is essential; however, mutations cbpB, gene encoding binding protein B, suppress essentiality. this study, we identified that reason for cbpB-dependent essentiality through induction stringent response by RelA....
Aromatic polyketides are renowned for their wide-ranging pharmaceutical activities. Their structural diversity is mainly produced via modification of limited types basic frameworks. In this study, we characterized the biosynthesis a unique aromatic framework, phenyldimethylanthrone (PDA) found in (+)/(−)-anthrabenzoxocinones (ABXs) and fasamycin (FAS). Its employs methyltransferase (Abx (+) M/Abx (−) M/FasT) an unusual TcmI-like aromatase/cyclase (ARO/CYC, Abx D/Abx D/FasL) as well...
MDC1 is a key mediator of the DNA-damage response in mammals with several phosphorylation-dependent protein interaction domains. The function its N-terminal forkhead-associated (FHA) domain remains elusive. Here, we show structural, biochemical and cellular data that FHA mediates dimerization to DNA damage. Crystal structures reveal face-to-face dimer pseudo-dyad symmetry. We found recognizes phosphothreonine 4 (pT4) at N-terminus determined crystal structure complex pT4 peptide. Biochemical...
Human indoleamine 2,3-dioxygenase 1 (IDO1) is a heme-dependent enzyme with important roles in many cellular processes and potential target for drug discovery against cancer other diseases. Crystal structures of IDO1 complex various inhibitors have been reported. Many these crystals belong to the same crystal form most reported resolutions range 3.2–2.3 Å. Here, three new forms human obtained by introducing surface mutation, K116A/K117A, distant from active site are One diffracted 1.5 Å...
Aromatic polyketides are an important class of natural products with various bioactivities, and their structural diversity arises from modifications to aromatic frameworks. In this study, we identify a stereoselective aromatase/cyclase (ARO/CYC) FasU, which is responsible for forming the axial chiral biphenyl framework in fasamycin. FasU catalyzes sequential decarboxylation cyclization/aromatization strict S-stereospecificity on previously unidentified biosynthetic intermediate. Through...
SUMMARY COP1 is a critical repressor of plant photomorphogenesis in darkness. However, plays distinct roles the photoreceptor UVR8 pathway Arabidopsis thaliana . interacts with ultraviolet B (UV‐B)‐activated monomers and promotes their retention accumulation nucleus. Moreover, has function UV‐B signaling, which involves binding its WD40 domain to HY5 via conserved Val‐Pro (VP) motifs these proteins. UV‐B‐activated both core VP motif, leading displacement from stabilization. it remains...
Abstract Pseudomonas species and other aerobic bacteria have a biotin-independent malonate decarboxylase that is crucial for their utilization of as the sole carbon energy source. The holoenzyme contains four subunits, having an acyl-carrier protein (MdcC subunit) with distinct prosthetic group, well (MdcD–MdcE) transferase (MdcA) catalytic activities. Here we report crystal structure hetero-tetramer, biochemical functional studies based on structural information. We observe molecule in...
C. elegans PUD-1 and PUD-2, two proteins up-regulated in daf-2(loss-of-function) (PUD), are homologous 17-kD with a large abundance increase long-lived daf-2 mutant animals of reduced insulin signaling. In this study, we show that both PUD-2 abundantly expressed the intestine hypodermis, form heterodimer. We have solved their crystal structure to 1.9-Å resolution found adopt similar β-sandwich folds V-shaped dimer. contrast, homologs PUD-3, PUD-4, PUDL-1 PUDL-2 all monomeric distinct...
Significance This work examines a fundamental question of how bacteria sense plant-released chemicals. We recently identified an effector one member plant-responsive PipR family transcription factors present in many plant-associated bacteria. compound (abbreviated HEHEAA) requires specific transporter for import into bacterial cells. have solved crystal structures component the free and bound to HEHEAA. discovered that close homolog protein cannot bind HEHEAA, implying there are other...
Gametogenesis is an essential step for malaria parasite transmission and activated in mosquito by signals including temperature drop, pH change, mosquito-derived xanthurenic acid (XA). Recently, a membrane protein gametogenesis 1 (GEP1) was found to be responsible sensing these interacting with giant guanylate cyclase α (GCα) activate the cGMP-PKG-Ca2+ signaling pathway gametogenesis. However, molecular mechanisms this process remain unclear. In study, we used AlphaFold2 predict structure of...
MU2 and bind by molecular sieving (View interaction)
A method for creating broadband ultrathin azo dye micropolarizers is introduced, utilizing photoalignment technology and liquid crystal polymers to organize dyes into a long-range ordered structure. Achievements include 3.63 μm thick polarizer with an extinction ratio over 140 polarization efficiency 99.3% across 400-650 nm, 5 × pixel pitch flexible checkerboard arrays 6 one-dimensional grating 0.45 duty through mask exposure technology. After substrate-transfer technique, the bending cycle...
Rabies virus is an enveloped negative-stranded RNA belonging to the family Rhabdoviridae.It can be successfully controlled by vaccination however, there are still tens of thousands deaths each year caused rabies due its mutations and complexity.A better understanding interaction between host might help solve this problem.Therefore, in study, we used two-dimensional electrophoresis investigate protein expression virus-infected mice.This us understand impact on during infection.For our...