A5012504037- Listeria monocytogenes in Food Safety
- Salmonella and Campylobacter epidemiology
- Microbial Inactivation Methods
- Vibrio bacteria research studies
- Essential Oils and Antimicrobial Activity
- Immune Response and Inflammation
- interferon and immune responses
- Bacterial biofilms and quorum sensing
- Viral Infectious Diseases and Gene Expression in Insects
- Immunotherapy and Immune Responses
- Escherichia coli research studies
- Yersinia bacterium, plague, ectoparasites research
- Bacteriophages and microbial interactions
- Probiotics and Fermented Foods
- Bacterial Genetics and Biotechnology
- Cancer Research and Treatments
- Bacillus and Francisella bacterial research
- Endoplasmic Reticulum Stress and Disease
- Cytokine Signaling Pathways and Interactions
- Inflammasome and immune disorders
- Cellular Mechanics and Interactions
- Autophagy in Disease and Therapy
- Microbial Fuel Cells and Bioremediation
- Nanoplatforms for cancer theranostics
- Viral gastroenteritis research and epidemiology
University of California, Berkeley
2016-2025
Atara Biotherapeutics (United States)
2025
Berkeley College
2020
Berkeley Public Health Division
2003-2017
University of Pennsylvania
1990-2014
Cornell University
2009
Institute of Molecular and Cell Biology
2000-2008
Cerus (United States)
2004-2007
Sidney Kimmel Cancer Center
2007
University of California, San Francisco
1998-2005
Listeria monocytogenes was used as a model intracellular parasite to study stages in the entry, growth, movement, and spread of bacteria macrophage cell line. The first step infection is phagocytosis Listeria, followed by dissolution membrane surrounding phagosome presumably mediated hemolysin secreted nonhemolytic mutants remain intact vacuoles. Within 2 h after infection, each now cytoplasmic becomes encapsulated actin filaments, identified such decoration filaments with subfragment 1...
Intracellular bacterial pathogens, such as Listeria monocytogenes, are detected in the cytosol of host immune cells. Induction this response is often dependent on microbial secretion systems and, L. multidrug efflux pumps (MDRs). Using monocytogenes mutants that overexpressed MDRs, we identified cyclic diadenosine monophosphate (c-di-AMP) a secreted molecule able to trigger cytosolic response. Overexpression di-adenylate cyclase, dacA (lmo2120), resulted elevated levels during infection....
Cyclic dinucleotide formulated cancer vaccine combined with PD-1 blockade can induce regression of tumors that do not express PD-L1 constitutively.
Yersinia enterocolitica isolates harboring a particular species of plasmid deoxyribonucleic acid showed high degree lethality for gerbils and caused the detachment HEp-2 tissue cell monolayers. Strains cured their loss these properties. However, invasiveness cells was shown not to be plasmid-mediated property. The expression plasmid-associated properties, including at least three major outer membrane polypeptides, occurred during growth 37 but 25 degrees C related concentration calcium in...
Type I interferons (IFNs) are central regulators of the innate and adaptive immune responses to viral bacterial infections. IFNs induced upon cytosolic detection microbial nucleic acids, including DNA, RNA, second messenger cyclic-di-GMP (c-di-GMP). In addition, a recent study demonstrated that intracellular pathogen Listeria monocytogenes stimulates type IFN response due bacterially secreted c-di-AMP. The transmembrane signaling adaptor Sting (Tmem173, Mita, Mpys, Eris) has recently been...
Actin filament assembly at the cell surface of pathogenic bacterium Listeria monocytogenes requires bacterial ActA protein and host Arp2/3 complex. Purified complex accelerated nucleation actin polymerization in vitro, but pure had no effect. However, when combined, synergistically stimulated filaments. This mechanism activating L. may be similar to strategy used by cells control activity hence spatial temporal distribution polymerization.
Two site-specific shuttle integration vectors were developed with two different chromosomal bacteriophage sites to facilitate strain construction in Listeria monocytogenes. The first vector, pPL1, utilizes the listeriophage U153 integrase and attachment site within comK gene for insertion. pPL1 contains a useful polylinker, can be directly conjugated from Escherichia coli into L. monocytogenes, forms stable, single-copy integrants at frequency of approximately 10(-4) per donor cell, used...
Summary The plcA gene of Listeria monocytogenes encodes a secreted phosphatidylinositol‐speciftc phospholi‐pase C (PI‐PLC). Recent studies have established that transposon mutations within result in avirulence for mice and pleiotropic effects when examined tissue‐culture models infection. Genetic analysis reveals many the insertions are due to loss readthrough transcription from into downstream prfA , which an essential factor numerous L. virulence genes. Construction in‐frame deletion had...
Listeria monocytogenes is a facultative intracellular pathogen that induces cytosolic signaling cascade resulting in expression of interferon (IFN)-β. Although type I IFNs are critical viral defense, their role immunity to bacterial pathogens much less clear. In this study, we addressed the by examining infection L. BALB/c mice lacking IFN receptor (IFN-α/βR−/−). During first 24 h vivo, IFN-α/βR−/− and wild-type were similar terms survival. addition, fate macrophages cultured from was...
Listeria monocytogenes secretes two distinct phospholipases C, a phosphatidylinositol-specific phospholipase C (PI-PLC) and broad-range (PC-PLC). In this study, single in-frame deletion mutants with mutations in each PLC double mutant lacking both PLCs were characterized regard to virulence mice, escape from primary vacuole, cell-to-cell spread cell culture. The PI-PLC, previously shown be twofold less virulent than the wild type had minor defect vacuole but was not notably affected spread....
Binding of antibodies to effector cells by way receptors their constant regions (Fc receptors) is central the pathway that leads clearance antigens immune system. The structure and function this important class on addressed through molecular characterization Fc (FcR) specific for murine immunoglobulin G isotype. Structural diversity encoded two genes alternative splicing result in expression molecules with highly conserved extracellular domains different transmembrane intracytoplasmic...
ABSTRACT For nearly 3 decades, listeriologists and immunologists have used mainly three strains of the same serovar (1/2a) to analyze virulence bacterial pathogen Listeria monocytogenes . The genomes two these strains, EGD-e 10403S, were released in 2001 2008, respectively. Here we report genome sequence third reference strain, EGD, extensive genomic phenotypic comparisons strains. Strikingly, is genetically highly distinct from EGD (29,016 single nucleotide polymorphisms [SNPs]) 10403S...