A5075261088- Mass Spectrometry Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Protein purification and stability
- Analytical Chemistry and Chromatography
- Monoclonal and Polyclonal Antibodies Research
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Fungal and yeast genetics research
- Biofuel production and bioconversion
- Glycosylation and Glycoproteins Research
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial Metabolic Engineering and Bioproduction
- Parasite Biology and Host Interactions
- Mathematical Dynamics and Fractals
- Cardiomyopathy and Myosin Studies
- Biosimilars and Bioanalytical Methods
- Reproductive Biology and Fertility
- Pesticide Residue Analysis and Safety
- Ion-surface interactions and analysis
- Molecular Biology Techniques and Applications
- Bioinformatics and Genomic Networks
- Spectroscopy Techniques in Biomedical and Chemical Research
- Biotin and Related Studies
- Cardiovascular Function and Risk Factors
- Sperm and Testicular Function
Roche (United States)
2023
Thermo Fisher Scientific (United States)
2007-2020
Bioanalytical Systems (United States)
2015
Thermo Fisher Scientific (Germany)
2015
Center of Immunology Pierre Fabre
2015
Institut Pluridisciplinaire Hubert Curien
2015
Université de Strasbourg
2015
Institute for Systems Biology
2009
University of Zurich
2009
ETH Zurich
2009
We have expanded our recent on-line LC−MS platform for large peptide analysis to combine collision-induced dissociation (CID), electron-transfer (ETD), and CID of an isolated charge-reduced (CRCID) species derived from ETD determine sites phosphorylation glycosylation modifications, as well the sequence fragments (i.e., 2000−10 000 Da) complex proteins, such β-casein, epidermal growth factor receptor (EGFR), tissue plasminogen activator (t-PA) at low femtomol level. The incorporation...
Myofilament proteins are responsible for cardiac contraction. The myofilament subproteome, however, has not been comprehensively analyzed thus far. In the present study, cardiomyocytes were isolated from rodent hearts and stimulated with endothelin-1 isoproterenol, potent inducers of protein phosphorylation. Subsequently, "skinned," subproteome was using a high mass accuracy ion trap tandem spectrometer (LTQ Orbitrap XL) equipped electron transfer dissociation. As expected, small number...
Spermiogenesis is a series of poorly understood morphological, physiological and biochemical processes that occur during the transition immotile spermatids into motile, fertilization-competent spermatozoa. Here, we identified Serpin (serine protease inhibitor) family protein (As_SRP-1) secreted from nematode Ascaris suum spermiogenesis (also called sperm activation) showed As_SRP-1 has two major functions. First, functions in cis to support (MSP)-based cytoskeletal assembly spermatid...
Rapid release of biopharmaceutical products enables a more efficient drug manufacturing process. Multi-attribute methods that target several product quality attributes (PQAs) at one time are an essential pillar the rapid-release strategy. The novel, high-throughput, and nondestructive multi-attribute Raman spectroscopy (MARS) method combines spectroscopy, design experiments, multivariate data analysis (MVDA). MARS allows measurement multiple PQAs for formulated protein therapeutics without...
New peak detection (NPD), as part of the LC-MS-based multi-attribute method (MAM), allows for sensitive and unbiased new or changing site-specific attributes between a sample reference that is not possible with conventional UV fluorescence detection-based methods. MAM NPD can serve purity test establish whether are similar. The broad implementation in biopharmaceutical industry has been limited by potential presence false positives artifacts, which increase analysis time trigger unnecessary...
Ergodic optimization for beta-transformations $T_\beta(x)= \beta x \pmod 1$ is developed. If $\beta>1$ a beta-number, or such that the orbit-closure of $1$ not minimal, we show Typically Periodic Optimization Conjecture holds, establishing there exists an open dense set H\"{o}lder continuous functions each function in this set, unique maximizing measure, measure supported on periodic orbit, and locking property holds. It follows typical among class beta-transformations: it holds parameters...
ABSTRACT An Mn 2+ and Cd uptake gene, mntA , was cloned from Lactobacillus plantarum ATCC 14917 into Escherichia coli . Its expression conferred on E. cells increased sensitivity as well energy-dependent activity. Both transcription translation of were induced by starvation in L. indicated reverse transcriptase PCR immunoblotting. Two systems have been identified : one is a high-affinity system that expressed -starved cells, the other nonsaturable -sufficient (Z. Hao, H. R. Reiske, D. B....
The use of electron-transfer dissociation as an alternative peptide ion activation method for generation protein sequence information is examined here in comparison with the conventional choice, collisionally activated dissociation, using a linear trapping instrument. Direct comparability between and electron-transfer-activated product data were ensured by employing activation-switching during acquisition, sequentially activating precisely same precursor species each fragmentation turn....
ABSTRACT Two different Cd 2+ uptake systems were identified in Lactobacillus plantarum . One is a high-affinity, high-velocity Mn system which also takes up and induced by starvation. The calculated K m V max are 0.26 μM 3.6 μmol g of dry cell −1 min , respectively. Unlike uptake, facilitated citrate related tricarboxylic acids, weakly inhibited citrate. competitive inhibitors each other, the affinity for higher than that other expressed -sufficient cells, no can be it because nonsaturable....
Tandem mass spectrometry in combination with liquid chromatography has emerged as a powerful tool for characterization of complex protein mixtures high-throughput manner. One the bioinformatics challenges posed by spectral data analysis is determination precursor charge when unit resolution used detecting fragment ions. The charge-state information to filter database sequences before they are correlated experimental data. In absence accurate state, several states assumed. This dramatically...
Better understanding how cells are regulated and adapt to their environment based on the reversible phosphorylation of proteins is a key question current molecular systems biology research. In this study, an advanced mass spectrometry approach leveraging electron transfer dissociation (ETD) technique in combination with CID using linear ion trap spectrometer described. The was applied, for first time, identification phosphorylated peptides isolated from Drosophila melanogaster Kc167 cell...
Filamentous fungi are intensively used for producing industrial enzymes, including lignocellulases. Employing insoluble cellulose to induce the production of lignocellulases causes some drawbacks, e.g., a complex fermentation operation, which can be overcome by using soluble inducers such as cellobiose. Here, triple β-glucosidase mutant Neurospora crassa, prevents rapid turnover cellobiose and thus allows disaccharide lignocellulases, was applied profile proteome responses (Avicel). Our...
Peptide mapping with liquid chromatography-tandem mass spectrometry (LC-MS/MS) is an important analytical method for characterization of post-translational and chemical modifications in therapeutic proteins. Despite its importance, there currently no consensus on the statistical analysis resulting data. In this manuscript, we distinguish three goals protein characterization: (1) estimation site occupancy one condition, (2) detection differential between conditions, (3) combined across...