A5039115975- Advanced biosensing and bioanalysis techniques
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- SARS-CoV-2 and COVID-19 Research
- RNA Research and Splicing
- RNA modifications and cancer
- Electrochemical sensors and biosensors
- Analytical chemistry methods development
- Advanced Photocatalysis Techniques
- Photodynamic Therapy Research Studies
- Luminescence and Fluorescent Materials
- Carbon and Quantum Dots Applications
- Monoclonal and Polyclonal Antibodies Research
- Mitochondrial Function and Pathology
- Calpain Protease Function and Regulation
- Electrochemical Analysis and Applications
- Analytical Chemistry and Sensors
- Infrastructure Maintenance and Monitoring
- Sulfur Compounds in Biology
- Traffic control and management
- RNA and protein synthesis mechanisms
- Nicotinic Acetylcholine Receptors Study
- Sarcoma Diagnosis and Treatment
- Acupuncture Treatment Research Studies
- Advanced Fiber Optic Sensors
Chongqing Institute of Green and Intelligent Technology
2012-2025
Nanjing University of Chinese Medicine
2022-2025
Chongqing Medical University
2025
Nankai University
2019-2024
Peking University
2020-2024
Beijing Academy of Artificial Intelligence
2024
China National Offshore Oil Corporation (China)
2024
Chinese University of Hong Kong, Shenzhen
2023
Northeast Forestry University
2021-2023
First Affiliated Hospital of Guangzhou Medical University
2020-2022
Reliable traffic prediction is critical to improve safety, stability, and efficiency of intelligent transportation systems. However, a very challenging problem because data are typical type spatio-temporal data, which simultaneously shows correlation heterogeneity both in space time. Most existing works can only capture the partial properties even assume that effect on globally invariable, resulting inadequate modeling unsatisfactory performance. In this paper, we propose novel end-to-end...
Protein self-assembly is one of the formation mechanisms biomolecular condensates. However, most phase-separating systems (PS) demand multiple partners in biological conditions. In this study, we divided PS proteins into two groups according to mechanism by which they undergo PS: PS-Self can self-assemble spontaneously form droplets, while PS-Part interact with PS. Analysis amino acid composition revealed differences sequence pattern between protein groups. Existing predictors, when...
Drug-induced liver injury (DILI) is the most common clinical adverse drug reaction, which closely associated with oxidative stress caused by overproduced reactive oxygen species. Hepatic H2O2, as an important biomarker of DILI, plays a crucial role in progression DILI. However, there remains challenge to develop H2O2-activatable second near-infrared (NIR-II, 1000–1700 nm) small molecular probes both large Stokes shift and long emission wavelength beyond 950 nm. Herein, we developed...
Lipid nanoparticles (LNPs) represent the most clinically advanced nonviral mRNA delivery vehicles; however, full potential of LNP platform is greatly hampered by inadequate endosomal escape capability. Herein, we rationally introduce a disulfide bond-bridged ester linker to modularly synthesize library 96 linker-degradable ionizable lipids (LDILs) for improved in vivo. The top-performing LDILs are composed one 4A3 amino headgroup, four linkers, and 10-carbon tail chains, whose unique...
Abstract It is challenging to develop a near‐infrared (NIR) small molecular photosensitizer for synergistic phototherapy in deep tissues. Herein, first, heavy‐atom‐free NIR hemicyanine (BHcy) 808 nm light‐mediated photodynamic therapy/photothermal therapy (PDT/PTT) anticancer by leveraging the acceptor engineering strategy reported. This endows BHcy with more planar and larger π‐conjugated structure, resulting long absorption/emission at 770/915–1200 as well enhanced singlet oxygen ( 1 O 2 )...
Conventional cyanine dyes with a symmetric structure are "always-on", which can easily accumulate in the liver and display high background fluorescence, inevitably interfering accurate diagnosis therapy extrahepatic diseases. We herein report platform of NIR-II non-symmetric (NSCyanine) by harnessing strategy, extremely sensitive to pH/viscosity be activated via "dual-key-and-lock" strategy. These NSCyanine low pKa (<4.0) only show weak fluorescence at lysosome pH (key1), however, completely...
Although activation of calcium-activated neutral protease (calpain) by the NMDA receptor has been suggested to play critical roles in synaptic modulation and neurologic disease, nature its substrates not completely defined. In this study, we examined ability calpain cleave cultured hippocampal neurons. Activation agonist application led rapid calpain-specific proteolysis spectrin decreased levels NR2A/2B subunits. Cleavage subunit created a 115 kDa product that retained bind 125 I-MK-801 is...
CTCF mediates chromatin insulation and long-distance enhancer-promoter (EP) interactions; however, little is known about how these regulatory functions are partitioned among target genes in key biological processes. Here, we show that Ctcf expression progressively increased during induced pluripotency. In this process, first as a insulator responsible for direct silencing of the somatic gene program and, interestingly, elevated next ensures accessibility contributes to EP interactions...
Monoclonal antibodies (mAbs) encoded by IGHV3-53 (VH3-53) targeting the spike receptor-binding domain (RBD) have been isolated from different COVID-19 patients. However, existence and prevalence of shared VH3-53-encoded in antibody repertoires is not clear. Using repertoire sequencing, we found that usage VH3-53 increased after SARS-CoV-2 infection. A highly VH3-53-J6 clonotype was identified 9 out 13 This derived convergent gene rearrangements with few somatic hypermutations evolutionary...
A comprehensive study of the B cell response against SARS-CoV-2 could be significant for understanding immune and developing therapeutical antibodies vaccines. To define dynamics characteristics antibody repertoire following infection, we analyzed mRNA transcripts immunoglobulin heavy chain (IgH) repertoires 24 peripheral blood samples collected between 3 111 days after symptom onset from 10 COVID-19 patients. Massive clonal expansion naive cells with limited somatic hypermutation (SHM) was...
Simultaneous detection of different diseases via a single fluorophore is challenging. We herein report bichromatic named Cy-914 for the simultaneous diagnosis non-alcoholic fatty liver disease (NAFLD) and metastatic intestinal cancer by leveraging its NIR-I/NIR-II dual-color imaging capability. with pKa 6.98 exhibits high sensitivity to pH viscosity, showing turn-on NIR-I fluorescence at 795 nm in an acidic tumor microenvironment, meanwhile displaying intense NIR-II 914/1030 under neutral...
The early detection of nonalcoholic fatty liver disease (NAFLD) through bioluminescent probes is great significance. However, there remains a challenge to apply them in nontransgenic natural animals due the lack exogenous luciferase. To address this issue, we herein report new strategy for situ monitoring endogenous hydrogen sulfide (H
Background Colorectal cancer (CRC) is a highly heterogeneous tumor, with significant variation in malignant cells, posing challenges for treatment and prognosis. However, this heterogeneity offers opportunities personalized therapy. Methods The consensus non-negative matrix factorization algorithm was employed to analyze single-cell transcriptomic data from CRC, which helped identify cell expression programs (MCEPs). Subsequently, crosstalk network linking MCEPs immune/stromal trajectory...
OpenComplex1 is an open-source platform for comprehending and developing biomolecular models. Based on the architectures of AlphaFold2 AlphaFold-Multimer, current version OpenComplex realizes end-to-end predictions three-dimensional (3D) structures including protein, RNA, protein-RNA complexes. makes it able to infer train models complexes in a unified framework supports encoders that use both MSAs protein language (pLM). Through extensive training structure sequence databases such as...
Abstract While protein aggregation’s association with aging and age-related diseases is well-established, the specific proteins involved whether dissolving them could alleviate remain unclear. Our research addresses this gap by uncovering role of PKM2 aggregates in aging. We find that forms senescent cells organs from aged mice, impairing its enzymatic activity glycolytic flux, thereby driving into senescence. Through a rigorous two-step small molecule library screening, we identify two...
ABSTRACT COVID-19, caused by SARS-CoV-2, is an acute self-resolving disease in most of the patients, but some patients can develop a severe illness or even death. To characterize host responses and identify potential biomarkers during progression, we performed longitudinal transcriptome analysis for peripheral blood mononuclear cells (PBMCs) collected from 4 COVID-19 at different time points symptom onset to recovery. We found that PBMCs stages exhibited unique characteristics. SARS-CoV-2...