A5024089474- Neuroscience and Neuropharmacology Research
- Cell death mechanisms and regulation
- Mitochondrial Function and Pathology
- Neuroinflammation and Neurodegeneration Mechanisms
- RNA regulation and disease
- Sirtuins and Resveratrol in Medicine
- RNA Interference and Gene Delivery
- Autophagy in Disease and Therapy
- Hydrogen Storage and Materials
- Telomeres, Telomerase, and Senescence
- Neurological Disease Mechanisms and Treatments
- Heme Oxygenase-1 and Carbon Monoxide
- Hemoglobinopathies and Related Disorders
- Ammonia Synthesis and Nitrogen Reduction
- Genomics and Chromatin Dynamics
- DNA Repair Mechanisms
- Adipose Tissue and Metabolism
- Epigenetics and DNA Methylation
- Blood properties and coagulation
- Immune Response and Inflammation
- Cancer-related gene regulation
- ATP Synthase and ATPases Research
- Cancer-related molecular mechanisms research
- Neurological Disorders and Treatments
- Ion channel regulation and function
Peking University
2014-2024
Weatherford College
2023
Shenzhen University
2023
Jilin University
2022
Air Force Medical University
2022
Zhejiang Chinese Medical University
2022
Yangtze University
2022
Peking University People's Hospital
2022
Guangdong Pharmaceutical University
2022
Sun Yat-sen Memorial Hospital
2022
Abstract: The observation that delayed death of CA1 neurons after global ischemia is inhibited by protein synthesis inhibitors suggests the these an active process requires new gene expression. Delayed in has some characteristics apoptotic death; however, candidate proapoptotic proteins have not been identified ischemia. We studied expression Bax and mRNA, a member bcl‐2 family effector cell death, four‐vessel model rat compared results with antiapoptotic . mRNA are both expressed before...
Abstract Peroxisome proliferator‐activated receptor gamma (PPAR‐γ) is a nuclear membrane‐associated transcription factor that governs the expression of various inflammatory genes. PPAR‐γ agonists protect peripheral organs from ischemic injury. In present study, we investigated whether agonist rosiglitazone neuroprotective against focal brain C57/B6 mice underwent 1.5‐h middle cerebral artery occlusion, and received either vehicle or treatment 0.75, 1.5, 3, 6 12 mg/kg ( n = 9 per group)....
Background and Purpose— Leptin is the major adipose hormone that regulates body weight energy expenditure by activating leptin receptors in hypothalamus. are also present other cell types, a potent antiapoptotic effect for has recently been reported. We investigated whether was neuroprotective against ischemic brain injury. Methods— In vitro injury induced rat primary neuronal culture oxygen-glucose deprivation 90 minutes. vivo middle cerebral artery occlusion mice 60 Results— were detected...
Abstract Targeted activation of highly ordered and distributed metal sites in nanoporous frameworks is a generic strategy to develop high‐performance catalysts. The key challenge achieve such without damaging the frameworks. Here it demonstrated that atmospheric‐pressure low‐temperature plasma generated air improve catalytic properties an Fe/Co bimetallic cyanide framework through specific “soft” incorporation reactive oxygen species affecting structure. bonding oxidative states high‐density...
Excitatory amino acids (EAAs) are important mediators of ischemic injury in stroke. N-Methyl-d-aspartate (NMDA) receptor antagonists have been shown to be very effective neuroprotective agents animal models stroke, but may unacceptable toxicity for human use. An alternative approach is inhibit the release EAAs during BW1003C87 [5-(2,3,5-trichlorophenyl)-2,4-diaminopyrimidine], a drug that inhibits veratrine-induced EAA glutamate vitro, was tested rat model proximal middle cerebral artery...
Hypoxic preconditioning (PC) confers robust neuroprotection against neonatal hypoxic-ischemic brain injury (H-I), yet the underlying mechanism is poorly understood. In adult brain, neuronal survival after ischemia associated with activation of phosphatidylinositol 3-kinase (PI3-K)/Akt signaling pathway. Suppression inflammation a newly identified direct consequence PI3-K/Akt signaling. We therefore investigated whether suppresses and contributes to PC-induced neuroprotection.Postnatal day 7...
Heme oxygenase-1 (HO-1) is an inducible Phase 2 enzyme that degrades toxic heme; its role in cerebral ischemia not fully understood. We hypothesize chemically induced HO-1 upregulation with the novel triterpenoid CDDO-Im (2-cyano-3,12 dioxooleana-1,9 dien-28-oyl imidazoline), a robust inducer of genes, protects neurons against ischemic injury.Using 3 different models ischemia, including oxygen-glucose deprivation neuronal cultures, global rats, and focal mice, we determined (1) whether...
Mitochondrial dysfunctions play major roles in many diseases. However, how mitochondrial stresses are relayed to downstream responses remains unclear. Here we show that the RNA component of mammalian telomerase TERC is imported into mitochondria, processed a shorter form TERC-53, and then exported back cytosol. We found import regulated by PNPASE, processing controlled mitochondrion-localized RNASET2. Cytosolic TERC-53 levels respond changes functions but have no direct effect on these...
Pentazole anion, the best candidate for full-nitrogen energetic materials, can be isolated only from acidic solution unclear reasons, which hinders high-yield realization of a substance with higher energy density. Herein, we report first time discovery dual aromaticity (π and σ) cyclo-N5-, makes anion unstable in nature but confers additional stability surroundings. In addition to usual π-aromaticity, similar that prototypical benzene, five lone pairs are delocalized equatorial plane forming...
Delayed hippocampal neurodegeneration after transient global ischemia is mediated, at least in part, through the activation of terminal caspases, particularly caspase-3, and subsequent proteolytic degradation critical cellular proteins. Caspase-3 may be activated by membrane receptor-initiated caspase-8–dependent extrinsic pathway mitochondria-initiated caspase-9–dependent intrinsic pathway; however, precise role these deduced apoptosis-signaling pathways activating caspase-3 ischemic...
Senescent cells display a senescence-associated secretory phenotype (SASP) which contributes to tumor suppression, aging, and cancer. However, the underlying mechanisms for SASP regulation are not fully elucidated. SIRT1, nicotinamide adenosine dinucleotide-dependent deacetylase, plays multiple roles in metabolism, inflammatory response, longevity, etc. its posttranscriptional cellular senescence still elusive. Here, we identify RNA-binding protein hnRNP A1 as regulator of well cell...
Abstract While protein aggregation’s association with aging and age-related diseases is well-established, the specific proteins involved whether dissolving them could alleviate remain unclear. Our research addresses this gap by uncovering role of PKM2 aggregates in aging. We find that forms senescent cells organs from aged mice, impairing its enzymatic activity glycolytic flux, thereby driving into senescence. Through a rigorous two-step small molecule library screening, we identify two...
Previous studies have shown that overexpression of bcl-2 in transgenic mice or by viral vectors protects the brain against cerebral ischemia. However, it is not known whether bcl-2, which endogenously expressed response to ischemia, exerts a protective effect. To address this question, authors blocked endogenous expression after ischemia using antisense oligodeoxynucleotides (ODN). Antisense, sense, scrambled ODN, vehicles were infused lateral ventricle rat for 24 hours 30 minutes temporary...
The synthesis and characterization of a series selective, orally bioavailable 1-(chroman-4-yl)urea TRPV1 antagonists is described. Whereas first-generation that inhibit all modes activation can elicit hyperthermia, the compounds disclosed herein do not elevate core body temperature in preclinical models only partially block acid TRPV1. Advancing SAR this led to eventual identification (R)-1-(7-chloro-2,2-bis(fluoromethyl)chroman-4-yl)-3-(3-methylisoquinolin-5-yl)urea (A-1165442, 52), an...
Sirtuin6(SIRT6) has been implicated as a key factor in aging and aging-related diseases. However, the role of SIRT6 cellular senescence not fully understood. Here, we show that repressed expression p27Kip1 (p27) senescence. The was reduced during senescence, whereas enforced promoted cell proliferation antagonized In addition, demonstrated p27 degradation by proteasome decreased acetylation level protein half-life p27. increased its stability. Furthermore, directly interacted with...
Abstract Human telomerase RNA component hTERC comprises multiple motifs that contribute to biogenesis, holoenzyme activity, and enzyme recruitment telomeres. contains several guanine tracts (G-tracts) at its 5′-end, but associated proteins potential roles in function are still poorly understood. The heterogeneous nuclear ribonucleoproteins F, H1, H2 (hnRNP F/H) splicing factors preferentially bind poly(G)-rich sequences RNA. Here, we demonstrate hnRNP F/H associate with both regulate...
In this study the relation between lysophosphatidic acid (LPA) and myocardial infarction was investigated, typical simplified methods for measuring serum LPA concentration by dot immunogold filtration assay (DIFA) based on a polyclonal antibody to were developed, concentrations measured in 31 patients with acute (AMI) 12 controls (blood donors) DIFA. Serum levels raised more than twofold 8 h after onset of AMI. Maximal elevation (10.43 mg/L) found at 48–72 following remained higher control...
Background and Purpose— Emerging evidence suggests that mitochondrial damage–mediated neuronal apoptosis is a major contributor to neonatal hypoxic-ischemic (H-I) brain injury. This study was performed determine whether targeted inhibition of the apoptotic protease activating factor-1 (Apaf-1) signaling pathway downstream damage confers neuroprotection in rodent models H-I. Methods— H-I induced 7-day-old (P7) transgenic mice overexpressing specific Apaf-1–inhibitory protein AIP. Apaf-1 also...
Microglia-involved neuroinflammation is thought to promote brain damage in various neurodegenerative disorders. Thus, inhibition of microglial over-activation may have a therapeutic benefit for the treatment Micheliolide (MCL) sesquiterpene lactone which inhibits inflammatory response. However, whether MCL can inhibit caused by LPS-activated BV2 microglia has not yet been explored. In this study, we demonstrated that cells with significantly repressed LPS-stimulated nitric oxide synthase...
Senescent cells display the senescence-associated secretory phenotype (SASP) which plays important roles in cancer, aging, etc. Cell surface-bound IL-1α is a crucial SASP factor and acts as an upstream regulator to induce NF-κB activity subsequent genes transcription.IL-1α exports cell surface via S100A13 protein-dependent non-classical pathway.However, status of this pathway during cellular senescence its role remain unknown.Here, we show that upregulated various types senescence.S100A13...
✓ Arachidonic acid metabolites are believed to be important mediators of tissue injury during reperfusion after cerebral ischemia. To determine whether inhibiting the oxygen-dependent metabolism arachidonic would reduce injury, we administered mixed cyclooxygenase—lipoxygenase inhibitor BW755C (3-amino-1-[ m (trifluoromethyl) phenyl]-2-pyrazoline) near time in a rat model temporary focal The duration ischemia + was 2 hours 22 hours, 3 or 21 hours. effects drug saline treatment on infarct...