A5100401271- Seismic Waves and Analysis
- Methane Hydrates and Related Phenomena
- Earthquake Detection and Analysis
- Seismology and Earthquake Studies
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- RNA Interference and Gene Delivery
- Platelet Disorders and Treatments
- Ion Channels and Receptors
- Retinal Diseases and Treatments
- Radio Frequency Integrated Circuit Design
- Caveolin-1 and cellular processes
- Cancer, Hypoxia, and Metabolism
- Advanced biosensing and bioanalysis techniques
- Signaling Pathways in Disease
- RNA modifications and cancer
- Protease and Inhibitor Mechanisms
- Cancer-related molecular mechanisms research
- Multimedia Communication and Technology
- Biochemical Analysis and Sensing Techniques
- MicroRNA in disease regulation
- Peptidase Inhibition and Analysis
- Glaucoma and retinal disorders
- Lymphatic System and Diseases
- Epigenetics and DNA Methylation
Army Medical University
2022-2025
Southwest Hospital
2025
Sun Yat-sen University
1993-2025
Nanjing University
2004-2025
Peking University
2011-2025
Fudan University
2020-2025
Zhongshan Hospital
2020-2025
Microbiology Institute of Shaanxi
2023-2025
Wenzhou Medical University
2024
Quzhou University
2024
Umami is one of the 5 basic taste qualities. The umami L-glutamate can be drastically enhanced by 5' ribonucleotides and synergy a hallmark this quality. receptor heteromeric complex 2 class C G-protein-coupled receptors, T1R1 T1R3. Here we elucidate molecular mechanism using chimeric T1R site-directed mutagenesis, modeling. We propose cooperative ligand-binding model involving Venus flytrap domain T1R1, where binds close to hinge region, bind an adjacent site opening further stabilize...
Zipping up obesity Chylomicrons are specialized particles that carry dietary fats from the intestine to bloodstream for absorption into body. Lacteals lymphatic vessels act as highway chylomicron transport, but it is unclear how passage occurs. Zhang et al. report two endothelial cell receptors, neuropilin-1 (NRP1) and vascular growth factor receptor 1 (VEGFR1, also known FLT1), required convert entry spaces between lacteals open junctions closed, zipped structures (see Perspective by...
Abstract Peroxisome proliferator‐activated receptor gamma (PPAR‐γ) is a nuclear membrane‐associated transcription factor that governs the expression of various inflammatory genes. PPAR‐γ agonists protect peripheral organs from ischemic injury. In present study, we investigated whether agonist rosiglitazone neuroprotective against focal brain C57/B6 mice underwent 1.5‐h middle cerebral artery occlusion, and received either vehicle or treatment 0.75, 1.5, 3, 6 12 mg/kg ( n = 9 per group)....
Background and Purpose— Leptin is the major adipose hormone that regulates body weight energy expenditure by activating leptin receptors in hypothalamus. are also present other cell types, a potent antiapoptotic effect for has recently been reported. We investigated whether was neuroprotective against ischemic brain injury. Methods— In vitro injury induced rat primary neuronal culture oxygen-glucose deprivation 90 minutes. vivo middle cerebral artery occlusion mice 60 Results— were detected...
Positive allosteric modulators of the human sweet taste receptor have been developed as a new way reducing dietary sugar intake. Besides their potential health benefit, enhancers are also valuable tool molecules to study general mechanism positive modulations T1R receptors. Using chimeric receptors, mutagenesis, and molecular modeling, we reveal how these work at level. Our data argue that follow similar natural umami enhancer molecules. Whereas sweeteners bind hinge region induce closure...
Activin receptor-like kinase 1 (ALK1) is an endothelial serine-threonine receptor for bone morphogenetic proteins (BMPs) 9 and 10. Inactivating mutations in the ALK1 gene cause hereditary haemorrhagic telangiectasia type 2 (HHT2), a disabling disease characterized by excessive angiogenesis with arteriovenous malformations (AVMs). Here we show that inducible, endothelial-specific homozygous Alk1 inactivation BMP9/10 ligand blockade both lead to AVM formation postnatal retinal vessels internal...
Background: Hereditary hemorrhagic telangiectasia (HHT) is an inherited vascular disorder that causes arteriovenous malformations (AVMs). Mutations in the genes encoding Endoglin ( ENG ) and activin-receptor-like kinase 1 AVCRL1 ALK1) cause HHT type 2, respectively. SMAD4 gene are present families with juvenile polyposis-HHT syndrome involves AVMs. a downstream effector of transforming growth factor-β (TGFβ)/bone morphogenetic protein (BMP) family ligands signal via activin-like receptors...
Abstract Endothelial cell migration, proliferation and survival are triggered by VEGF-A activation of VEGFR2. However, how these behaviors regulated individually is still unknown. Here we identify Endophilin-A2 (ENDOA2), a BAR-domain protein that orchestrates CLATHRIN-independent internalization, as critical mediator endothelial migration sprouting angiogenesis. We show EndoA2 knockout mice exhibit postnatal angiogenesis defects impaired front-rear polarization tip cells. ENDOA2 deficiency...
Pulmonary hypertension (PH) is associated with increased expression of VEGF-A (vascular endothelial growth factor A) and its receptor, VEGFR2 2), but whether how activation signal participates in the pathogenesis PH unclear.VEGF-A/VEGFR2 Y949-dependent vascular leak were investigated lung samples from patients mice exposed to hypoxia. To study their mechanistic roles hypoxic PH, we examined right ventricle systolic pressure, ventricular hypertrophy, pulmonary vasculopathy mutant carrying...
Thiamethoxam (THM) is one of the most widely used insecticides globally, which was designed to selectively target nicotinic acetylcholine receptors (nAChRs) in insect nervous system and generally considered safe for non-targeted organisms. However, increasing evidence has demonstrated its neurotoxicity aquatic organisms, though underlying mechanisms, especially at environmentally relevant concentrations, remain largely unclear. In this study, swimming distance zebrafish significantly...
A design methodology for low power MOS distributed amplifiers (DAs) is presented. The bias point of the devices optimized so that DA can be used as a low-noise amplifier (LNA) in broadband applications. prototype 9-mW LNA with programmable gain was implemented 0.18-/spl mu/m CMOS process. provides flat gain, S/sub 21/, 8 /spl plusmn/ 0.6dB from DC to 6.2 GHz, an input impedance match, 11/, -16 dB and output 22/, -10 over entire band. 3-dB bandwidth 7GHz, IIP3 +3 dBm, noise figure ranges 4.2...
Sprouting angiogenesis is a key process driving blood vessel growth in ischemic tissues and an important drug target number of diseases, including wet macular degeneration wound healing. Endothelial cells forming the sprout must develop front-rear polarity to allow extension. The adaptor proteins Nck1 2 are known regulators cytoskeletal dynamics polarity, but their function poorly understood. Here, we show that Nck adaptors required for endothelial cell migration downstream angiogenic...
Stroke is the third leading cause of death worldwide and a huge perpetrator in adult disability. This pilot clinical study investigates possible benefits transplanting multiple cells chronic stroke. A total 10 consecutive stroke patients were treated by combination cell transplantation on basis an intraparenchymal approach from November 2003 to April 2011. There six males four females. Their age ranged 42 87 years, course disease varied 6 months 20 years. Six suffered cerebral infarction,...
Lymphatic vessels are responsible for tissue drainage, and their malfunction is associated with chronic diseases. Lymph uptake occurs via specialized open cell-cell junctions between capillary lymphatic endothelial cells (LECs), whereas closed in collecting LECs prevent lymph leakage. LEC known to dynamically remodel development disease, but how permeability regulated remains poorly understood.
The integrin alpha3beta1 mediates cellular adhesion to the matrix ligand laminin-5. A second ligand, urokinase receptor (uPAR), associates with via a surface loop within alpha3 beta-propeller (residues 242-246) but outside laminin binding region, suggesting that uPAR-integrin interactions could signal differently from engagement. To explore this, alpha3-/- epithelial cells were reconstituted wild-type (wt) or Ala mutations uPAR-interacting (H245A R244A). Wt mutant-bearing showed comparable...
Up-regulation of urokinase receptors is common during tumor progression and thought to promote invasion metastasis. Urokinase bind a set beta1 integrins, but it remains unclear what degree receptor/integrin binding important integrin signaling. Using site-directed mutagenesis, single amino acid mutants the receptor were identified that fail associate with either alpha3beta1 (D262A) or alpha5beta1 (H249A) normally urokinase. To study effects these mutations on function, endogenous first...
The TRPV1 channel is a detector of noxious stimuli, including heat, acidosis, vanilloid compounds and lipids. gating mechanisms the related TRPV2 are poorly understood because selective high affinity ligands not available, threshold for heat activation extremely (>50°C). Cryo-EM structures reveal that they adopt similar structures, identify putative binding pocket near internal side TRPV1. Here we use biochemical electrophysiological approaches to investigate resiniferatoxin(RTx) site in...
Bis(diphenylphorothioyl)amide (Stpip) containing phosphor–sulfur (P═S) bonds was used as an ancillary ligand for three pure green iridium(III) emitters Ir1, Ir2, and Ir3, which were synthesized in few minutes at room temperature with high reaction yields above 70%. All these complexes show good thermal stability excellent sublimation of around 80–90%, are considered beneficial industry practical production organic light-emitting diode (OLED) fabrication. The emission profiles meet the...
Abstract Vascular permeability and neovascularization are implicated in many diseases including retinopathies diabetic wound healing. Robo4 is an endothelial-specific transmembrane receptor that stabilizes the vasculature, as shown −/− mice develop hyperpermeability, but how signals remained unclear. Here we show deletion enhances revascularization oxygen-induced retinopathy (OIR) accelerates cutaneous To determine signalling pathways, generated transgenic expressing a truncated lacking...
A design methodology is presented for low power distributed amplifiers and used the of a 9 mW LNA with programmable gain implemented in 0.18 /spl mu/m CMOS process. The provides 8 plusmn/ 0.6 dB from DC to 6.2 GHz, an input match -16 output -10 over entire band. IIP3 +1.8 dBm, NF ranges 4.2 dB. tunable +8 while flatness matching are maintained.