A5035288415- Neurogenesis and neuroplasticity mechanisms
- Axon Guidance and Neuronal Signaling
- Cerebrospinal fluid and hydrocephalus
- Angiogenesis and VEGF in Cancer
- Lymphatic System and Diseases
- MicroRNA in disease regulation
- Zebrafish Biomedical Research Applications
- Epigenetics and DNA Methylation
- Spinal Dysraphism and Malformations
- Traumatic Brain Injury and Neurovascular Disturbances
- Glioma Diagnosis and Treatment
- Congenital heart defects research
- Neuroinflammation and Neurodegeneration Mechanisms
- Intracerebral and Subarachnoid Hemorrhage Research
- Immune cells in cancer
- Developmental Biology and Gene Regulation
- Fetal and Pediatric Neurological Disorders
- Advanced Neuroimaging Techniques and Applications
- Pluripotent Stem Cells Research
- Neonatal Respiratory Health Research
- Nerve injury and regeneration
- Hippo pathway signaling and YAP/TAZ
- Genetic and Kidney Cyst Diseases
- Long-Term Effects of COVID-19
- SARS-CoV-2 and COVID-19 Research
Sorbonne Université
2016-2025
Institut du Cerveau
2015-2025
Pitié-Salpêtrière Hospital
1997-2024
Yale University
2015-2024
Inserm
2013-2024
Centre National de la Recherche Scientifique
2012-2022
Institut de Psychiatrie et Neurosciences de Paris
2022
Assistance Publique – Hôpitaux de Paris
2011-2021
Allen Institute for Brain Science
2020
University of Manchester
2020
Although COVID-19 is considered to be primarily a respiratory disease, SARS-CoV-2 affects multiple organ systems including the central nervous system (CNS). Yet, there no consensus on consequences of CNS infections. Here, we used three independent approaches probe capacity infect brain. First, using human brain organoids, observed clear evidence infection with accompanying metabolic changes in infected and neighboring neurons. However, for type I interferon responses was detected. We...
The recent discovery of meningeal lymphatic vessels (LVs) has raised interest in their possible involvement neuropathological processes, yet little is known about development or maintenance. We show here that LVs develop postnatally, appearing first around the foramina basal parts skull and spinal canal, sprouting along blood cranial nerves to various meninges surrounding central nervous system (CNS). VEGF-C, expressed mainly vascular smooth muscle cells, VEGFR3 endothelial cells were...
Glioblastoma is one of the most angiogenic human tumours and endothelial proliferation a hallmark disease. A better understanding glioblastoma vasculature needed to optimize anti-angiogenic therapy that has shown high but transient efficacy. We analysed tissues found non-endothelial cell-lined blood vessels were formed by tumour cells (vasculogenic mimicry tubular type). hypothesized CD133+ presenting stem-cell properties may express pro-vascular molecules allowing them form de novo....
Vascular sprouting is a key process-driving development of the vascular system. In this study, we show that neuropilin-2 (Nrp2), transmembrane receptor for lymphangiogenic endothelial growth factor C (VEGF-C), plays an important role in lymphatic vessel sprouting. Blocking VEGF-C binding to Nrp2 using antibodies specifically inhibits developing tip cells vivo. vitro analyses modulates cell extension and prevents stalling retraction during sprout formation. Genetic deletion reproduces defects...
Vascular remodeling under conditions of growth or exercise, during recovery from arterial restriction blockage is essential for health, but mechanisms are poorly understood. It has been proposed that endothelial cells have a preferred level fluid shear stress, ‘set point’, determines remodeling. We show human umbilical vein respond optimally within range stress approximate physiological shear. Lymphatic cells, which experience much lower flow in vivo, similar effects at value stress. VEGFR3...
Summary Although COVID-19 is considered to be primarily a respiratory disease, SARS-CoV-2 affects multiple organ systems including the central nervous system (CNS). Yet, there no consensus whether virus can infect brain, or what consequences of CNS infection are. Here, we used three independent approaches probe capacity brain. First, using human brain organoids, observed clear evidence with accompanying metabolic changes in infected and neighboring neurons. However, for type I interferon...
Meningeal lymphatic vessels (MLVs) were identified in the dorsal and caudobasal regions of dura mater, where they ensure waste product elimination immune surveillance brain tissues. Whether MLVs exist anterior part murine human skull how connect with glymphatic system extracranial lymphatics remained unclear. Here, we used light-sheet fluorescence microscopy (LSFM) imaging mouse whole-head preparations after OVA-A555 tracer injection into cerebrospinal fluid (CSF) performed real-time...
Abstract The eye, an anatomical extension of the central nervous system (CNS), exhibits many molecular and cellular parallels to brain. Emerging research demonstrates that changes in brain are often reflected particularly retina 1 . Still, possibility immunological nexus between posterior eye rest CNS tissues remains unexplored. Here, studying immune responses herpes simplex virus brain, we observed intravitreal immunization protects mice against intracranial viral challenge. This protection...
Meningeal lymphatic vessels (MLVs) promote tissue clearance and immune surveillance in the central nervous system (CNS). Vascular endothelial growth factor-C (VEGF-C) regulates MLV development maintenance has therapeutic potential for treating neurological disorders. Herein, we investigated effects of VEGF-C overexpression on brain fluid drainage ischemic stroke outcomes mice. Intracerebrospinal administration an adeno-associated virus expressing mouse full-length (AAV-mVEGF-C) increased CSF...
The plp gene encodes the proteolipid protein and its alternatively spliced product DM-20, major proteins of CNS myelin. In mouse, plp/dm-20 transcripts are expressed beginning at embryonic day 9.5 (E9.5) by restricted foci germinative neuroepithelial cells. To determine identity neural precursors expressing plp/dm- 20 , a zeomycin resistance fused to lacZ reporter was in transgenic mice under control regulatory sequences. three different lines generated, pattern β-galactosidase expression...
Oligodendrocytes, the myelin-forming cells of CNS, are generated from multiple foci distributed along developing neural tube. Little is known about endogenous guidance cues controlling migration oligodendrocyte precursor (OPCs) their site emergence toward final destination, mainly future white matter tracts. During embryonic development, optic nerve populated by OPCs originating in diencephalon that migrate chiasm retina. Here we show migrating into express semaphorin receptors neuropilin-1...
Netrins are secreted molecules with roles in axonal growth and angiogenesis. The Netrin receptor UNC5B is required during embryonic development for vascular patterning, suggesting that it may also contribute to postnatal pathological Here we show unc5b down-regulated quiescent adult vasculature, but re-expressed sprouting angiogenesis matrigel tumor implants. Stimulation of UNC5B-expressing neovessels an agonist (Netrin-1) inhibits Genetic loss function reduces Netrin-1-mediated inhibition....
The presence of demyelinated plaques in the central nervous system is hallmark multiple sclerosis (MS). Some remyelinate but others do not, leaving permanent damage. reasons for this failure repair are many, one possible reason lack migration oligodendrocyte precursor cells to lesion. guidance molecules Semaphorin 3A and 3F, already known direct oligodendroglial development, may also be active controlling cell MS, hence determine ability remyelinate. Here, MS tissue an experimental model...
Neural stem cells (NSCs) are slowly dividing astrocytes that intimately associated with capillary endothelial in the subventricular zone (SVZ) of brain. Functionally, members vascular growth factor (VEGF) family can stimulate neurogenesis as well angiogenesis, but it has been unclear whether they act directly via VEGF receptors (VEGFRs) expressed by neural cells, or indirectly release factors from angiogenic capillaries. Here, we show VEGFR-3, a receptor required for lymphangiogenesis, is...
Oligodendrocytes are the myelin-forming cells of CNS. They differentiate from oligodendrocyte precursor (OPCs) that produced progenitors throughout life but more actively during neonatal period and in response to demyelinating insults. An accurate regulation oligodendrogenesis is required generate oligodendrocytes these developmental or repair processes. We hypothesized this implicates transcription factors, which expressed by OPCs and/or their progenitors. Ascl1/Mash1 a proneural factor...
Rationale: The lymphatic vasculature plays a major role in fluid homeostasis, absorption of dietary lipids, and immune surveillance. Fluid transport depends on the presence intraluminal valves within collectors. Defective formation leads to lymphedema, progressive debilitating condition for which curative treatments are currently unavailable. How valve is regulated remains largely unknown. Objective: We investigated if repulsive axon guidance molecule Semaphorin3A (Sema3A) formation. Methods...
Gene expression levels vary across developmental stage, cell type, and region in the brain. Genomic variants also contribute to variation expression, some neuropsychiatric disorder loci may exert their effects through this mechanism. To investigate these relationships, we present BrainVar, a unique resource of paired whole-genome bulk tissue RNA sequencing from dorsolateral prefrontal cortex 176 individuals prenatal postnatal development. Here identify common that alter gene (expression...
The blood–brain barrier (BBB) is partially disrupted in brain tumors. Despite the gaps BBB, there an inadequate amount of pharmacological agents delivered into brain. Thus, low delivery efficiency renders many these ineffective treating cancer. In this report, we proposed "autocatalytic" approach for increasing transport nanoparticles strategy, a small number enter via transcytosis or through BBB gaps. After penetrating release modulators, which enables more to be transported, creating...