A5039186754- Genomics and Phylogenetic Studies
- Identification and Quantification in Food
- Microbial Community Ecology and Physiology
- Neurogenesis and neuroplasticity mechanisms
- Mesenchymal stem cell research
- RNA Interference and Gene Delivery
- Neuroscience and Neuropharmacology Research
- Cytokine Signaling Pathways and Interactions
- Hematopoietic Stem Cell Transplantation
- interferon and immune responses
- RNA Research and Splicing
- Pluripotent Stem Cells Research
- CAR-T cell therapy research
- Functional Brain Connectivity Studies
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- Plant Pathogens and Fungal Diseases
- Monoclonal and Polyclonal Antibodies Research
- Cancer-related molecular mechanisms research
- Bacterial Identification and Susceptibility Testing
- Single-cell and spatial transcriptomics
- Fungal and yeast genetics research
- Neuroendocrine regulation and behavior
- Genetic Associations and Epidemiology
- Nerve injury and regeneration
Yale University
2018-2025
Institute of Microbial Technology
2013-2025
Central University of Punjab
2025
Thermo Fisher Scientific (United States)
2014-2024
Lovely Professional University
2023
SUNY Old Westbury
2022
South Dakota State University
2016-2019
Savitribai Phule Pune University
2010-2018
National Centre for Cell Science
2010-2018
Ames National Laboratory
2016
This article reports on the application of organically modified silica (ORMOSIL) nanoparticles as a nonviral vector for efficient in vivo gene delivery. Highly monodispersed, stable aqueous suspension nanoparticles, surface-functionalized with amino groups binding DNA, were prepared and characterized. Stereotaxic injections complexed plasmid DNA encoding EGFP, into mouse ventral midbrain lateral ventricle, allowed us to fluorescently visualize extensive transfection neuronal-like cells...
This article reports a multidisciplinary approach to produce fluorescently labeled organically modified silica nanoparticles as nonviral vector for gene delivery and biophotonics methods optically monitor intracellular trafficking transfection. Highly monodispersed, stable aqueous suspensions of nanoparticles, encapsulating fluorescent dyes surface functionalized by cationic-amino groups, are produced micellar nanochemistry. Gel-electrophoresis studies reveal that the particles efficiently...
MicroRNAs (miRNAs) are increasingly being recognized as involved in cancer development and progression gliomas.Using a model cell system developed our lab to study glioma comprising human neuroglial culture (HNGC)-1 HNGC-2 cells, we report here that miR-145 is one of the miRNAs significantly downregulated during malignant transformation glioblastoma multiforme (GBM). In using tumor samples derived from various grades, show expression decreased graded manner, with GBM patients showing lowest...
Gene expression levels vary across developmental stage, cell type, and region in the brain. Genomic variants also contribute to variation expression, some neuropsychiatric disorder loci may exert their effects through this mechanism. To investigate these relationships, we present BrainVar, a unique resource of paired whole-genome bulk tissue RNA sequencing from dorsolateral prefrontal cortex 176 individuals prenatal postnatal development. Here identify common that alter gene (expression...
Abstract The ventrolateral pallial (VLp) excitatory neurons in the claustro-amygdalar complex and piriform cortex (PIR; which forms part of palaeocortex) form reciprocal connections with prefrontal (PFC), integrating cognitive sensory information that results adaptive behaviours 1–5 . Early-life disruptions these circuits are linked to neuropsychiatric disorders 4–8 , highlighting importance understanding their development. Here we reveal transcription factors SOX4, SOX11 TFAP2D have a...
Abstract Generation of reactive oxygen species (ROS) with the accumulation oxidative damage has been implicated in neurodegenerative disease and degradation nervous system function age. Here we report that ROS inhibit activity ciliary neurotrophic factor (CNTF) nerve cells. Treatment hydrogen peroxide (H 2 O ) as a generator inhibited CNTF‐mediated Jak/STAT signaling all cultured cells tested, including chick ganglion neurons, neural retina, HMN‐1 motor neuron hybrid cells, SH‐SY5Y BE(2)‐C...
The yeast vacuole is the storage depot for cellular iron. In this report we quantify import-export balance in because of import iron by Ccc1p and to export combined activity Smf3p ferroxidase, permease pair proteins, Fet5p Fth1p. Our data indicate that two efflux pathways are equally efficient trafficking out vacuole. A major focus work was identify ferrireductase(s) supplies FeII whether or Fet5p-Fth1p complex. Using a combination flameless atomic absorption spectrophotometry vacuolar whole...
Background & aimsCell therapies based on mesenchymal stromal cells (MSCs) have gained an increasing therapeutic interest in the context of multiple disorders. Nonetheless, this field still faces important challenges, particularly concerning suitable manufacturing platforms. Here, we aimed at establishing a scalable culture system to expand umbilical cord-derived Wharton's jelly MSC (MSC(WJ)) and their derived extracellular vesicles (EVs) by using dissolvable microcarriers combined with...
The high affinity iron uptake complex in the yeast plasma membrane (PM) consists of ferroxidase, Fet3p, and ferric permease, Ftr1p. We used a combination two-hybrid analysis, confocal fluorescence microscopy, resonance energy transfer (FRET) quantification to delineate motifs two proteins required for assembly maturation into an uptake-competent complex. cytoplasmic, carboxyl-terminal domain each protein contains four-residue motif adjacent cytoplasm-PM interface that supports interaction...
Mammals have evolved a plethora of adaptations that enabled them to thrive in diverse environments. Among the most significant is emergence more complex brain, exemplified by dramatic transformation dorsal cortex from single layer excitatory projection neurons (ExNs) ancestors multilayered cerebral neocortex enriched with intratelencephalic (IT) and extratelencephalic (ET) ExN subtypes. These ExNs established specialized systems, such as corticospinal tract corpus callosum, enhancing brain...
Long noncoding RNAs constitute a major fraction of the eukaryotic transcriptome, and together with proteins, they intricately fine-tune various growth regulatory signals to control cellular homeostasis. Here, we describe functional characterisation novel pair long intergenic (lincRNAs) comprised complementary, fully overlapping sense antisense transcripts Genomic Instability Inducing RNA (Ginir) Ginir (Giniras), respectively, from mouse cells. This transcript is expressed in spatiotemporal...
Augmentor α and β (Augα Augβ) are newly discovered ligands of the receptor tyrosine kinases Alk Ltk. Augα functions as a dimeric ligand that binds with high affinity specificity to However, monomeric fragment Augβ also bind potently stimulate cellular responses. While previous studies demonstrated oncogenic mutants function important drivers variety human cancers, physiological roles poorly understood. Here, we investigate by exploring mice deficient in each or both Aug ligands. Analysis...
Abstract Amycolatopsis regifaucium, a Gram-positive actinomycete, is prolific source of biologically active compounds, including polyphenol antibiotics like kigamicins. This study presents the draft genome regifaucium DSM 45072T (= GY080T), which spans 8.28 Mbp and assembled into 62 contigs, with annotation revealing 312 subsystems, 7,966 coding genes, 52 RNAs, GC content 68.5 mol%. We found significant genomic diversity within genus, variations in core accessory elements across species....
A novel Gram-staining-negative gammaproteobacterium, designated IITR-13T, was isolated from a pesticide-contaminated soil and characterized using polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain showed closest similarity (98.7 %) to Rheinheimera tangshanensis JA3-B52T followed by texasensis A62-14BT (97.7 soli BD-d46T (97.3 %). The other members genus < 97.3 %. However, DNA-DNA hybridization between IITR-13T type strains R. tangshanensis, 47.5 ± 0.6, 42.4 0.4 39.8...
Neurite outgrowth is an important morphological phenotype of neuronal cells that correlates with their function and cell health, yet there are limited methods available for measuring this phenomenon. Current approaches to neurite laborious time-consuming, relying largely upon immunocytochemical staining markers (e.g., beta-III tubulin or MAP2) followed by manual automated microscopy image acquisition analysis. Here we report the development a quick simple dual-color fluorescent dye-based...
The taxonomic position of a Gram-stain positive bacterium isolated from solar saltern sample collected Kanyakumari, coastal region the Bay Bengal, India, was analysed by using polyphasic approach. strain, designated SA2-6T, had phenotypic characteristics that matched those genus Bacillus. 16S rRNA gene sequence (1493 bases) novel strain compared with previously studied Bacillus type strains and confirmed belongs to moderately closely related foraminis at 97.5 % similarity, followed...