A5005149073- Immune Cell Function and Interaction
- IL-33, ST2, and ILC Pathways
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- SARS-CoV-2 detection and testing
- Cancer Immunotherapy and Biomarkers
- Long-Term Effects of COVID-19
- Cytomegalovirus and herpesvirus research
- COVID-19 Clinical Research Studies
- Reproductive System and Pregnancy
- Inflammasome and immune disorders
- Biosensors and Analytical Detection
- COVID-19 diagnosis using AI
- Autoimmune and Inflammatory Disorders Research
- Advanced biosensing and bioanalysis techniques
- CAR-T cell therapy research
- Toxoplasma gondii Research Studies
- Immune cells in cancer
- interferon and immune responses
- HIV Research and Treatment
- Dental Research and COVID-19
- DNA Repair Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Telomeres, Telomerase, and Senescence
Memorial Sloan Kettering Cancer Center
2016-2023
Yale University
2020-2023
Kettering University
2019
Hebrew University of Jerusalem
2013
Although COVID-19 is considered to be primarily a respiratory disease, SARS-CoV-2 affects multiple organ systems including the central nervous system (CNS). Yet, there no consensus on consequences of CNS infections. Here, we used three independent approaches probe capacity infect brain. First, using human brain organoids, observed clear evidence infection with accompanying metabolic changes in infected and neighboring neurons. However, for type I interferon responses was detected. We...
Abstract Rapid and accurate SARS-CoV-2 diagnostic testing is essential for controlling the ongoing COVID-19 pandemic. The current gold standard diagnosis real-time RT-PCR detection of from nasopharyngeal swabs. Low sensitivity, exposure risks to healthcare workers, global shortages swabs personal protective equipment, however, necessitate validation new approaches. Saliva a promising candidate diagnostics because (1) collection minimally invasive can reliably be self-administered (2) saliva...
Summary Although COVID-19 is considered to be primarily a respiratory disease, SARS-CoV-2 affects multiple organ systems including the central nervous system (CNS). Yet, there no consensus whether virus can infect brain, or what consequences of CNS infection are. Here, we used three independent approaches probe capacity brain. First, using human brain organoids, observed clear evidence with accompanying metabolic changes in infected and neighboring neurons. However, for type I interferon...
Significance Telomeres protect the ends of eukaryotic chromosomes. shorten with age and serve as a biological clock that limits cell proliferation. Excessive telomere shortening accelerates aging, but elongation may facilitate cancer. We found inherited mutations in regulator helicase 1 (RTEL1), which cause Hoyeraal–Hreidarsson syndrome, fatal disease characterized by accelerated shortening, immunodeficiency, developmental defects. Introducing normal RTEL1 gene into affected cells prevented...
Abstract The recent spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) exemplifies the critical need for accurate and rapid diagnostic assays to prompt clinical public health interventions. Currently, several quantitative reverse-transcription polymerase chain reaction (qRT-PCR) are being used by clinical, research, laboratories. However, it is currently unclear if results from different tests comparable. Our goal was evaluate primer-probe sets in four common available on...
Runx1 and Runx3 play nonredundant roles in promoting the clonal expansion of NK cells during viral infection.
Autophagy is an essential cellular survival mechanism that required for adaptive lymphocyte development; however, its role in innate lymphoid cell (ILC) development remains unknown. Furthermore, the conditions promote autophagy during homeostasis are poorly understood. Here, we demonstrate Atg5, component of machinery, mature natural killer (NK) cells and group 1, 2, 3 ILCs. Although inducible ablation Atg5 was dispensable precursors lymphocytes lymphoreplete mice, found induced both...
Interleukin-18 (IL-18) promotes natural killer (NK) and T cell production of interferon (IFN)-γ, a key factor in resistance to Toxoplasma gondii, but previous work has shown limited role for endogenous IL-18 control this parasite. Although infection with T. gondii results release IL-18, the IFN-γ induces high levels binding protein (IL-18BP). Antagonism IL-18BP "decoy-to-the-decoy" (D2D) construct that does not signal rather binds enhanced innate lymphoid (ILC) responses improved parasite...
Latent viral infections rely on a precise coordination of the immune response to control sporadic reactivation. CD8+ T cells play crucial role in controlling latency by generating diverse memory responses an epitope-specific manner. Among these distinct responses, conventional and inflationary have been described during herpesvirus infections. Using newly generated TCR transgenic mouse strain, we investigated transcriptomic epigenetic remodeling CMV infection across tissues at both...
Despite robust secondary T cell expansion primed by vaccination, the impact on primary immune responses to heterotypic antigens remains undefined. Here we show that of epitope-specific memory CD8+ cells prior infection with recombinant pathogens limits naive specificity new heterologous antigens, dampening protective immunity against subsequent pathogen challenge. The degree repression directly paralleled magnitude recall response. Suppressed priming reflects competition for antigen...
The progression of transformed primary tumors to metastatic colonization is a lethal determinant disease outcome. Although circulating adaptive and innate lymphocyte effector responses are required for effective antimetastatic immunity, whether tissue-resident immune circuits confer initial immunity at sites dissemination remains ill defined. Here we examine the nature local cell during early seeding in lung using intracardiac injection mimic monodispersed spread. Using syngeneic murine...
The ability of immune cells to directly interact with transformed is an essential component surveillance and critical for optimal tissue function. tumor–immune interactome (the collective cellular interactions between oncogenic cells) distinct varied based on the location immunogenicity tumor subtypes. However, comprehensive landscape consequences tumor-interacting in microenvironment are not well understood. Current tools limited their identify record interactors vivo or be utilized...
Abstract Adaptive lymphocyte clones with the greatest receptor affinity tend to dominate primary and secondary immune responses. Strength of signal abundance, along competition for antigen cytokines, drive preferential outgrowth certain clones, shaping effector memory pool in selection processes known as avidity maturation. Natural killer (NK) cells are innate lymphocytes capable “adaptive” responses following infectious challenges. However, whether NK undergo a similar process on basis...