A5101515280- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Phytase and its Applications
- Epigenetics and DNA Methylation
- Video Surveillance and Tracking Methods
- Folate and B Vitamins Research
- Cancer-related gene regulation
- Single-cell and spatial transcriptomics
- Data Management and Algorithms
- Face and Expression Recognition
- Congenital heart defects research
- Cancer-related molecular mechanisms research
- Genetics and Neurodevelopmental Disorders
- Neurogenesis and neuroplasticity mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Advanced Image and Video Retrieval Techniques
- Renal and related cancers
- Face recognition and analysis
- Protein Degradation and Inhibitors
- Multimodal Machine Learning Applications
- Oral and gingival health research
- Human Pose and Action Recognition
- Advanced biosensing and bioanalysis techniques
Shanxi University
2011-2025
Dalian Maritime University
2023-2024
Guangzhou Medical University
2017-2023
Guangzhou Institutes of Biomedicine and Health
2013-2023
First Affiliated Hospital of Guangzhou Medical University
2023
State Key Laboratory of Respiratory Disease
2023
First Hospital of China Medical University
2020-2021
Southern California University for Professional Studies
2021
University of Southern California
2021
China Medical University
2020
Unsupervised hashing methods have attracted widespread attention with the explosive growth of large-scale data, which can greatly reduce storage and computation by learning compact binary codes. Existing unsupervised attempt to exploit valuable information from samples, fails take local geometric structure unlabeled samples into consideration. Moreover, based on auto-encoders aims minimize reconstruction loss between input data codes, ignores potential consistency complementarity multiple...
Incomplete multi-view clustering primarily focuses on dividing unlabeled data into corresponding categories with missing instances, and has received intensive attention due to its superiority in real applications. Considering the influence of incomplete data, existing methods mostly attempt recover by adding extra terms. However, for unsupervised methods, a simple recovery strategy will cause errors outlying value accumulations, which affect performance methods. Broadly, previous have not...
Abstract Polycomb repressive complex 1 (PRC1) is an important regulator of gene expression and development. PRC1 contains the E3 ligases RING1A/B, which monoubiquitinate lysine 119 at histone H2A (H2AK119ub1), has been sub-classified into six major complexes based on presence a PCGF subunit. Here, we report that PCGF5, one paralogs, requirement in differentiation mouse embryonic stem cells (mESCs) towards neural cell fate. Although PCGF5 not required for mESC self-renewal, its loss blocks by...
R-loops modulate genome stability and regulate gene expression, but the functions regulatory mechanisms of in stem cell biology are still unclear. Here, we profiled during somatic reprogramming found that dynamic changes essential for occurred before expression. Disrupting homeostasis by depleting RNaseH1 or catalytic inactivation at D209 (RNaseH1D209N) blocks reprogramming. Sox2, not any other factor Yamanaka cocktail, overcomes inhibitory effects activity loss on Sox2 interacts with...
Super-enhancers (SEs) are cis-regulatory elements enriching lineage specific key transcription factors (TFs) to form hotspots. A paucity of identification and functional dissection promoted us investigate SEs during myoblast differentiation. ChIP-seq analysis histone marks leads the uncovering which remodel progressively course Further analyses TF enable definition SE hotspots co-bound by master TF, MyoD other TFs, among we perform in-depth for MyoD/FoxO3 interaction in driving formation...
Mutations in the Crumbs homolog 2 (CRB2) gene cause various autosomal recessive genetic diseases, such as leber congenital amaurosis, retinitis pigmentosa and ventriculomegaly with cystic kidney disease. However, precise roles of CRB2 cell fate determination remains unknown. Here, we generated a homozygous knockout (CRB2-/-) H1 human embryonic stem cells (hESCs) using CRISPR/Cas9 system. This line maintained normal morphology karyotype, expressed pluripotency makers. Importantly, has ability...
CCCTC-binding factor (CTCF) is a ubiquitously expressed "master weaver" and plays multiple functions in the genome, including transcriptional activation/repression, chromatin insulation, imprinting, X chromosome inactivation, high-order organization. It has been shown that CTCF facilitates recruitment of upstream binding onto ribosomal DNA (rDNA) regulates local epigenetic state rDNA repeats. However, mechanism by which modulates rRNA gene transcription not well understood. Here we found...
High-throughput genetic screening is useful for discovering critical genes or gene sequences that trigger specific cell functions and/or phenotypes. Loss-of-function mainly achieved through RNA interference (RNAi), CRISPR knock-out (CRISPRko), and (CRISPRi) technologies. Gain-of-function depends on the overexpression of a cDNA library activation (CRISPRa). Base editing can perform both gain- loss-of-function screening. This review discusses techniques based Cas9 nuclease, including...
Diabetic wounds that do not heal for a long time challenge global healthcare. Mesenchymal stem cell (MSC) therapy has positive significance in promoting diabetic wound healing. However, traditional MSC involves exogenous MSCs, which brings many limitations and unsatisfactory treatment. Moreover, the maintenance of viability function is difficult because high level reactive oxygen species (ROS) wounds. Therefore, we developed nanofibrous dressing to recruit protect endogenous MSCs while...
Polycomb repressive complex 1 (PRC1) comprises two different complexes: CBX-containing canonical PRC1 (cPRC1) and RYBP/YAF2-containing variant (vPRC1). RYBP-vPRC1 or YAF2-vPRC1 catalyzes H2AK119ub through a positive-feedback model; however, whether RYBP YAF2 have regulatory functions is still unclear. Here, we show that the expression of decreases increases, respectively, during neural differentiation embryonic stem cells (ESCs). Rybp knockout impairs by activating Wnt signaling derepressing...
Abstract Nucleosome positioning and histone modification play a critical role in gene regulation, but their during reprogramming has not been fully elucidated. Here, we determined the genome-wide nucleosome coverage methylation occupancy mouse embryonic fibroblasts (MEFs), induced pluripotent stem cells (iPSCs) pre-iPSCs. We found that increases promoter regions decreases intergenic pre-iPSCs, then recovers to an intermediate level iPSCs. also nucleosomes pre-iPSCs are much more phased than...
Purpose . In our study, we aimed to screen the risk factors that affect overall survival (OS) and cancer-specific (CSS) in adult glioma patients develop evaluate nomograms. Methods Primary high-grade gliomas being retrieved from surveillance, epidemiology end results (SEER) database, between 2004 2015, then they randomly assigned a training group validation group. Univariate multivariate Cox analysis models were used choose variables significantly correlated with prognosis of patients. And...
Abstract Induced pluripotent stem cells can be derived from somatic through ectopic expression of transcription factors or chemical cocktails. Chemical iPSCs (C-iPSCs) and OSKM-iPSCs (4F-iPSCs) have been suggested to similar characteristics mouse embryonic (mESCs). However, their epigenetic equivalence remains incompletely understood throughout the genome. In this study, we generated C-iPSCs 4F-iPSCs, further compared genome-wide DNA methylomes C-iPSCs, mESCs that were maintained in 2i LIF....
A total of 31 differentially expressed genes in the mammary glands were identified our previous study using RNA sequencing (RNA-Seq), for lactating cows with extremely high and low milk protein fat percentages. To determine regulation composition traits, we herein investigated expression profiles microRNA (miRNA) small based on same samples as RNA-Seq experiment. 497 known miRNAs (miRBase, release 22.1) 49 novel among reads identified. Among these miRNAs, 71 found between groups ( p <...