A5090837645- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Pregnancy and preeclampsia studies
- Chronic Obstructive Pulmonary Disease (COPD) Research
- CRISPR and Genetic Engineering
- Reproductive System and Pregnancy
- Epigenetics and DNA Methylation
- Pluripotent Stem Cells Research
- Endometriosis Research and Treatment
- Neonatal Respiratory Health Research
- Estrogen and related hormone effects
- Angiogenesis and VEGF in Cancer
- RNA Research and Splicing
- MicroRNA in disease regulation
- Hemoglobinopathies and Related Disorders
- Cancer, Hypoxia, and Metabolism
- Pancreatic function and diabetes
- Adipokines, Inflammation, and Metabolic Diseases
- Single-cell and spatial transcriptomics
- interferon and immune responses
- Cancer Research and Treatments
- FOXO transcription factor regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Endometrial and Cervical Cancer Treatments
- Cancer, Lipids, and Metabolism
Hainan Medical University
2019-2025
Hainan Center for Disease Control & Prevention
2024
University of Virginia
2020-2024
East China Normal University
2023
Fourth Hospital of Hebei Medical University
2020
Hebei Medical University
2020
Johns Hopkins University
2020
Office of Public Health Genomics
2020
West China Medical Center of Sichuan University
2019
Ocean University of China
2017
Understanding pancreas development can provide clues for better treatments of pancreatic diseases. However, the molecular heterogeneity and developmental trajectory early human are poorly explored. Here, we performed large-scale single-cell RNA sequencing assay transposase accessible chromatin embryonic tissue obtained from first-trimester embryos. We unraveled heterogeneity, trajectories regulatory networks major cell types. The results reveal that dorsal multipotent cells in humans exhibit...
Abstract The lung is the primary respiratory organ in human, which proximal airway and distal alveoli are responsible for air conduction gas exchange, respectively. However, regulation of proximal–distal patterning at embryonic stage human development largely unknown. Here we investigated early embryos weeks 4–8 post fertilization (Carnegie stages 12–21) using single-cell RNA sequencing, obtained a transcriptomic atlas 169,686 cells. We observed discernible gene expression patterns epithelia...
<i>Objective:</i> To establish the transfection method of vascular endothelial growth factor (VEGF) gene into mesenchymal stem cells (MSCs), to investigate effect this gene-transfected MSCs for heart function restoration and angiogenesis after myocardial infarction, compare therapeutic differences among cell therapy, combined therapy. <i>Methods:</i> Ischemic models were constructed in inbred Wistar rats by ligation left anterior descending coronary artery. isolated...
Abstract Chronic obstructive pulmonary disease (COPD), diagnosed by reduced lung function, is a leading cause of morbidity and mortality. We performed whole genome sequence (WGS) analysis function COPD in multi-ethnic sample 11,497 participants from population- family-based studies, 8499 individuals COPD-enriched studies the NHLBI Trans-Omics for Precision Medicine (TOPMed) Program. identify at genome-wide significance 10 known GWAS loci 22 distinct, previously unreported loci, including two...
KDM5D is a gene implicated in spermatogenic failure and sex-related differences colon cancer progression, though its role spermatogenesis remains unclear. We successfully generated knockout human embryonic stem cells using CRISPR/Cas9 technology. This cell line provides valuable model for studying KDM5D's function influence on sex various diseases.
The dynein cytoplasmic two heavy chain 1 (DYNC2H1) gene encodes a subunit. Cytoplasmic dyneins transport cargo towards the minus end of microtubules and are thus termed "retrograde" cellular motor. Mutations in DYNC2H1 main causative mutations short rib-thoracic dysplasia syndrome type III with or without polydactyly (SRTD3). Early diagnosis SRTD3 prenatally by ultrasound alone is difficult. In this case, couple who gave birth to three consecutive babies requested fertility guidance avoid...
RNA-binding proteins (RBPs) are key players of gene expression and perturbations RBP-RNA regulatory network have been observed in various cancer types. Here, we propose a computational method, RBPreg, to identify the RBP regulators by integration single cell RNA-Seq (N = 233,591) binding data. Pan-cancer analyses suggest that exhibit specificity involved hallmark-related functions. We prioritize an oncogenic RBP-HNRNPK, which is highly expressed tumors associated with poor prognosis...
Dysregulated epithelial-mesenchymal transition (EMT) is involved in cervical cancer metastasis and associated with histone acetylation. However, the underlying molecular mechanisms of acetylation EMT are still elusive.We systematically investigated expression patterns genes their correlations pathway cancer. The CSRP2BP among tissues cell lines was detected using Western blotting immunohistochemistry analyses. effects on proliferation tumorigenicity were examined by growth curve, EdU assay,...
Y chromosome deletion and karyotype abnormalities are commonly associated with congenital non-obstructive azoospermia, impairing spermatogenesis. Specifically, the of Azoospermia factor a (AZFa) has been identified in infertile males severely impaired AZFa, encompassing megabase-scale region, poses challenges modeling AZFa deletion-related male infertility using gene editing tools. Here, we successfully created an AZFa-deleted human embryonic stem cell line utilizing CRISPR/Cas9 tool. Our...
Abstract Background Fibrogenesis within ovarian endometrioma (endometrioma), mainly induced by transforming growth factor-β (TGF-β), is characterized myofibroblast over-activation and excessive extracellular matrix (ECM) deposition, contributing to endometrioma-associated symptoms such as infertility impairing reserve oocyte quality. However, the precise molecular mechanisms that underpin endometrioma- associated fibrosis progression TGF-β remain poorly understood. Methods The expression...
Malignant growth and chemotherapy resistance to 5-fluorouracil (5-FU) are the obstacles treatment of Colorectal cancer (CRC). There is need develop effective therapeutic option. Demethylzeylasteral benefits immune anti-tumor function. However, role demethylzeylasteral played in colorectal remains unclear. Here, our study confirmed that could inhibit cell malignant capacity, such as proliferation, migration invasion. And we also found cause cycle arrest apoptosis. Followed verified...
Abstract Early‐onset preeclampsia (PE) is a pregnancy complication that can lead to severe adverse maternal and fetal outcomes. However, the mechanisms underlying development of early‐onset PE are not fully understood. Ribosomal protein L39 (RPL39) member S39E family ribosomal proteins plays an important role in stem cell self‐renewal, cancer metastasis, chemoresistance. In this study, we aimed explore potential function RPL39 placental trophoblast cells. We analyzed expression normal...
Large-scale cancer genome sequencing has enabled the catalogs of somatic mutations; however, mutational impact on intrinsically disordered protein regions (IDRs) not been systematically investigated to date. Here, we comprehensively characterized landscapes IDRs and found that have higher mutation frequencies across diverse cancers. We thus developed a computational method, ROI-Driver, identify putative driver genes enriching IDR domain hotspots in cancer. Numerous well-known cancer-related...
Background The replication of coronaviruses takes place on cytoplasmic double membrane vesicles (DMVs) originating in the endoplasmic reticulum (ER). Three trans-membrane non-structural proteins, nsp3, nsp4 and nsp6, are understood to be anchors coronavirus complex. Nsp4 is localized ER when expressed alone but recruited into complex infected cells. It revealed contain four regions its N- C-termini exposed cytosol. Methodology/Principal Findings We have determined crystal structures...
Abstract β-thalassemia is a condition characterized by reduced or absent synthesis of β-globin resulting from genetic mutations, leading to expanded and ineffective erythropoiesis. Mitoxantrone has been widely used clinically as an antitumor agent considering its ability inhibit cell proliferation. However, therapeutic effect on erythropoiesis in untested. We found that mitoxantrone decreased α-globin precipitates ameliorated anemia, splenomegaly, the HbbTh3/+ mouse model intermedia. The...
Gastric cancer (GC) is one of the major public health problems worldwide with high morbidity and mortality. Nowadays, traditional medicine may hold promise for treatment cancers. Gossypol-acetic acid (GAA) a male contraceptive agent that shows anti-tumor effects on multiple types However, whether GAA would inhibit progression GC remained unclear.The potential targets were predicted by Pharmmapper software GC-related genes obtained from GeneCard database. The "GC-targets-GAA" network was...
Rationale: Chronic obstructive pulmonary disease (COPD) is a complex characterized by airway obstruction and accelerated lung function decline. Our understanding of systemic protein biomarkers associated with COPD remains incomplete. Objectives: To determine what proteins pathways are impaired in diverse population. Methods: We studied 6,722 participants across six cohort studies both aptamer-based proteomic spirometry data (4,566 predominantly White discovery analysis 2,156 African American...
Background: The heterogeneity and dynamic changes of endometrial cells have a significant impact on health as they determine the normal function endometrium during menstrual cycle. Dysfunction can lead to occurrence various gynecological diseases. Therefore, deconvolution immune microenvironment that drives transcriptional programs throughout cycle is key understand regulatory biology endometrium. Methods: Herein, we comprehensively analyzed single-cell transcriptome 59,397 across ten human...
The spoilage of beer by bacteria is great concern to the brewer as this can lead turbidity and abnormal flavors. polymerase chain reaction (PCR) method for detection beer-spoilage highly specific provides results much faster than traditional microbiology techniques. However, one drawbacks inability differentiate between live dead cells. In paper, combination propidium monoazide (PMA) pretreatment conventional PCR had been described. established PMA-PCR identified Lactobacillus brevis based...