A5100677210- Machine Learning in Bioinformatics
- Image Processing Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- RNA and protein synthesis mechanisms
- Cancer Mechanisms and Therapy
- Immunotherapy and Immune Responses
- Pluripotent Stem Cells Research
- Lung Cancer Treatments and Mutations
- CRISPR and Genetic Engineering
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- T-cell and B-cell Immunology
- Angiogenesis and VEGF in Cancer
- Epigenetics and DNA Methylation
- NF-κB Signaling Pathways
- Cancer-related molecular mechanisms research
- Connective Tissue Growth Factor Research
- Peptidase Inhibition and Analysis
- MicroRNA in disease regulation
- Bone and Joint Diseases
- Iron Metabolism and Disorders
- Inflammasome and immune disorders
- Pulmonary Hypertension Research and Treatments
- Therapeutic Uses of Natural Elements
- FOXO transcription factor regulation
Hainan Medical University
2024-2025
First Affiliated Hospital of Bengbu Medical College
2024-2025
Nanjing Medical University
2021-2025
Hainan Center for Disease Control & Prevention
2024
Ruijin Hospital
2024
Shanghai Jiao Tong University
2024
Shandong University
2019-2024
Air Force Medical University
2017-2024
Lanzhou University Second Hospital
2024
Lanzhou University
2024
KDM5D is a gene implicated in spermatogenic failure and sex-related differences colon cancer progression, though its role spermatogenesis remains unclear. We successfully generated knockout human embryonic stem cells using CRISPR/Cas9 technology. This cell line provides valuable model for studying KDM5D's function influence on sex various diseases.
ABSTRACT Objective Despite growing interest in the gut microbiota and blood metabolome patients with ankylosing spondylitis (AS), its role remains poorly understood. Here, we investigate how microbial metabolic alterations contribute to AS. Methods Fecal microbiome data from 40 AS were compared those healthy controls (HCs) using 16S ribosomal RNA (rRNA) gene sequencing. The plasma profiles analyzed integrated identify biological characteristics specific Results showed significant enrichment...
Lipopolysaccharide/d-galactosamine (LPS/GalN)-induced hepatic injury is an experimental model of fulminant failure in which tumor necrosis factor alpha (TNF-α) plays a pivotal role. Moreover, it was reported from our laboratory that interleukin (IL) 17A enhanced production TNF-α by the Kupffer cell.The purpose this study to determine role IL-17A LPS/GalN-induced mice.LPS/GalN injected into three mouse models: wild-type (WT) mice, knockout (KO) or KO mice treated with recombinant (rm)...
Intrahepatic cholangiocarcinoma (IHCC) is an aggressive cancer with a poor survival rate and the second most common type of primary hepatobiliary system. At present, molecular mechanisms IHCC initiation progression remain unclear. Recent evidence has indicated that long non‑coding RNAs (lncRNAs) serve crucial role in development; however, functional lncRNAs not been investigated detail. In present study, marked overexpression lncRNA colon cancer‑associated transcript 2 (CCAT2) was observed...
Radiotherapy is frequently applied for clinically localized prostate cancer while its efficacy could be significantly hindered by radioresistance.MicroRNAs (miRNAs) are important regulators in mediating cellular responses to ionizing radiation (IR), and strongly associate with radiosensitivity many cancers.In this study, enhancement of miR-29b-3p was demonstrated cell line LNCaP vitro.Results showed that expression upregulated response IR from both X-rays carbon ion irradiations.Knockdown...
Abstract Ongoing chronic fibrosis and hypertrophy of the ligamentum flavum (LF) is an important cause lumbar spinal canal stenosis (LSCS). Our previous work showed that WNT1-inducible signaling pathway protein 1 (WISP-1) a critical driver LF fibrosis. However, potential mechanism has not been explored. Here, we found Gli1 was upregulated in hypertrophic tissues required for fibrogenesis fibroblasts. Moreover, mechanical stretching increased expression WISP-1 Furthermore, induced vitro...
Y chromosome deletion and karyotype abnormalities are commonly associated with congenital non-obstructive azoospermia, impairing spermatogenesis. Specifically, the of Azoospermia factor a (AZFa) has been identified in infertile males severely impaired AZFa, encompassing megabase-scale region, poses challenges modeling AZFa deletion-related male infertility using gene editing tools. Here, we successfully created an AZFa-deleted human embryonic stem cell line utilizing CRISPR/Cas9 tool. Our...
To investigate the role of macrophage colony-stimulating factor (M-CSF) in patients with hepatocellular carcinoma (HCC) after surgery.Expression M-CSF, distribution M2 macrophages (MΦs), and angiogenesis were assessed liver, including tumors peritumoral liver tissues. The prognostic power these factors was assessed. Mouse isolated hepatic MΦs or monocytes cultured media containing M-CSF. concentration vascular endothelial growth (VEGF) Furthermore, M-CSF-matured on proliferation cell (VEC)...
Abstract Objective Alterations in gut microbiota have been implicated the pathogenesis of ankylosing spondylitis (AS), but underlying mechanisms remain elusive. This study aims to investigate changes and metabolites individuals with AS before after treatment secukinumab, identify biological characteristics specific patients potential biomarkers, for optimizing therapeutic strategies more effectively. Methods Fecal microbiome data were collected from 30 secukinumab therapy compared 40 healthy...
Hypertrophy of ligamentum flavum (LF) is a significant contributing factor to lumbar spinal canal stenosis (LSCS). lncRNA plays vital role in organ fibrosis, but its LF fibrosis remains unclear. Our previous findings have demonstrated that Hedgehog-Gli1 signaling critical driver leading hypertrophy. Through the RIP experiment, our group found lnc-RMRP was physically associated with Gli1 and exhibited enrichment Gli1-activated cells. Histological studies revealed elevated expression RMRP...
// Xin Meng 1, * , Jianping Li 2, Minjuan Zheng 1 Lei Zuo Chao Sun Yongsheng Zhu Ling Fang Liwen Liu Xiaodong Zhou Department of Ultrasonography, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, Shaanxi, China 2 Radiation Oncology, These authors contributed equally to this work Correspondence to: Zhou, email: zhouxdfmmu@tom.com Liu, liulwus@163.com Keywords: gold nanorods (AuNRs), hepatosis, stable peptide, LSD1, human mesenchymal stem cell (human MSCs) Received: January...
Background: Forkhead box C1 (FOXC1) is an important cancer-associated gene in tumor. PPAR-γ and C/EBPα are both transcriptional regulators involved tumor development. Objective: We aimed to clarify the function of PPAR-γ, hepatocellular carcinoma (HCC) relationship FOXC1 HCC. Methods: Western blotting, immunofluorescent staining, immunohistochemistry were used evaluate protein expression. qRT-PCR was assess mRNA Co-IP performed detect interaction. And ChIP fluorescent reporter detection...
Abstract Muscle regeneration after damage or during myopathies requires a fine cooperation between myoblast proliferation and myogenic differentiation. A growing body of evidence suggests that microRNAs play critical roles in myocyte differentiation transcriptionally. However, the molecular mechanisms underlying orchestration are not fully understood. Here, we showed miR-130b is able to repress promote via targeting Sp1 transcription factor. Importantly, overexpression capable improving...
The present study aimed to observe the content difference of macrophage migration inhibitory factor [MIF; novoprotein recombinant human MIF (n‑6his) (ch33)], TGFβ1 and MMP13 in patients with without ligamentum flavum (LF) hypertrophy investigate roles LF hypertrophy. concentration MIF, were detected by ELISA a lumbar spinal stenosis (LSS) group disc herniation (LDH) group. Culture primary LFs identification performed for subsequent study. Cell treatments cell proliferation assay CCK‑8 was...
Downregulated DSC2 involved in the metastasis of cancers. Unfortunately, its role on development gastric cancer (GC) and potential mechanisms remain unclear. Bioinformatics analysis, Western blot, qRT-PCR, immunohistochemistry were performed to detect levels human GC normal stomach tissues. The downstream signaling carcinogenesis explored by using specimens, cells with different expression, inhibitors, mouse models. We found that level decreased significantly tissues cells. Recovered...