A5100364318- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Video Surveillance and Tracking Methods
- Genomics, phytochemicals, and oxidative stress
- Receptor Mechanisms and Signaling
- Kruppel-like factors research
- Wnt/β-catenin signaling in development and cancer
- MicroRNA in disease regulation
- TGF-β signaling in diseases
- Dental Implant Techniques and Outcomes
- Genetic Syndromes and Imprinting
- interferon and immune responses
- Neuropeptides and Animal Physiology
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Histone Deacetylase Inhibitors Research
- Cancer-related molecular mechanisms research
- Molecular Biology Techniques and Applications
- Electrochemical Analysis and Applications
- Dental Radiography and Imaging
- Electrochemical sensors and biosensors
- Telomeres, Telomerase, and Senescence
- UAV Applications and Optimization
Shandong University
2023-2024
Xuzhou Medical College
2023-2024
Hunan Children's Hospital
2024
Central South University
2024
Yangzhou University
2024
Jiangxi Normal University
2024
State Key Laboratory of Natural Medicine
2019-2023
Anhui University of Science and Technology
2023
Sichuan University
2013-2023
State Key Laboratory of Oral Diseases
2016-2023
DNA methylation at the C-5 position of cytosine (5mC) is one best-studied epigenetic modifications and plays important roles in diverse biological processes. Iterative oxidation 5mC by ten-eleven translocation (Tet) family proteins generates 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxylcytosine (5caC). 5fC 5caC are selectively recognized excised thymine glycosylase (TDG), leading to demethylation. Functional characterization Tet has been complicated redundancy between...
Modern crowd counting methods usually employ deep neural networks (DNN) to estimate counts via density regression. Despite their significant improvements, the regression-based are incapable of providing detection individuals in crowds. The detection-based methods, on other hand, have not been largely explored recent trends due needs for expensive bounding box annotations. In this work, we instead propose a new network with only point supervision required. It can simultaneously detect size...
Abstract Age-related osteoporosis is characterized by the deterioration in bone volume and strength, partly due to dysfunction of marrow mesenchymal stromal/stem cells (MSCs) during aging. Alpha-ketoglutarate (αKG) an essential intermediate tricarboxylic acid (TCA) cycle. Studies have revealed that αKG extends lifespan worms maintains pluripotency embryonic stem (ESCs). Here, we show administration increases mass aged mice, attenuates age-related loss, accelerates regeneration rodents....
The Keap1-Nrf2-ARE pathway regulates the constitutive and inducible transcription of various genes that encode detoxification enzymes, antioxidant proteins anti-inflammatory has pivotal roles in defence against cellular oxidative stress. In this study, we investigated therapeutic potential CPUY192018, a potent small-molecule inhibitor Keap1-Nrf2 protein-protein interaction (PPI), renal inflammation. human proximal tubular epithelial HK-2 cells, CPUY192018 treatment significantly increased...
ALKBH1 was recently discovered as a demethylase for DNA N6-methyladenine (N6-mA), new epigenetic modification, and interacts with the core transcriptional pluripotency network of embryonic stem cells. However, role N6-mA in regulating osteogenic differentiation is largely unknown. In this study, we demonstrated that expression human mesenchymal cells (MSCs) upregulated during induction. Knockdown increased genomic levels significantly reduced osteogenic-related genes, alkaline phosphatase...
Abstract Objectives Growth differentiation factor 11 (GDF11), an emerging secreted member of the TGF‐beta superfamily, plays essential roles in development, physiology and multiple diseases; however, its role during adipogenic underlying mechanisms remains poorly understood. Materials methods Bone marrow‐derived human mesenchymal stem cells (hMSCs) 3T3‐L1 pre‐adipocytes were induced with culture medium supplementing different concentrations recombinant GDF11 (rGDF11 0, 10, 50, 100 ng mL −1...
Polycomb repressive complex 1 (PRC1) plays essential roles in cell-fate determination. Recent studies have found that the composition of mammalian PRC1 is particularly varied and complex; however, little known about functional consequences these variant complexes on Here, we show Kdm2b promotes Oct4-induced somatic reprogramming through recruitment a (PRC1.1) to CpG islands (CGIs). Furthermore, find bone morphogenetic protein (BMP) represses Oct4/Kdm2b-induced selectively. Mechanistically,...
Dysregulated lineage commitment of mesenchymal stem cells (MSCs) contributes to impaired bone formation and an imbalance between adipogenesis osteogenesis during skeletal aging osteoporosis. The intrinsic cellular mechanism that regulates MSC remains unclear. Here, we identified Cullin 4B (CUL4B) as a critical regulator commitment. CUL4B is expressed in marrow MSCs (BMSCs) downregulated with mice humans. Conditional knockout Cul4b resulted postnatal development low mass reduced formation....
Unmanned aerial vehicle (UAV)-based tracking is attracting increasing attention and developing rapidly in applications such as agriculture, aviation, navigation, transportation public security. Recently, discriminative correlation filters (DCF)-based trackers have stood out UAV community for their high efficiency appealing robustness on a single CPU. However, due to limited onboard computation resources other challenges the accuracy of existing DCF-based approaches still not satisfying. In...
Family B G protein-coupled receptors (GPCRs) play vital roles in hormone-regulated homeostasis. They are drug targets for metabolic diseases, including type 2 diabetes and osteoporosis. Despite their importance, the signaling mechanisms family GPCRs at molecular level remain largely unexplored due to challenges purification of functional sufficient amount biophysical characterization. Here, we purified GPCR human glucagon-like peptide-1 (GLP-1) receptor (GLP1R), whose agonists, e.g....
DNA N6-adenine methylation (6mA), as a novel adenine modification existing in eukaryotes, shows essential functions embryogenesis and mitochondrial transcriptions. ALKBH1 is demethylase of 6mA plays critical roles osteogenesis, tumorigenesis, adaptation to stress. However, the integrated biological still require further exploration. Here, we demonstrate that knockdown inhibits adipogenic differentiation both human mesenchymal stem cells (hMSCs) 3T3-L1 preadipocytes, while overexpression...
It was reported that circular RNAs (circRNAs) and microRNAs (miRNAs) were related to non-small cell lung cancer (NSCLC) development. However, the detailed mechanisms of circ_0020123 miR-384 in NSCLC are elusive.QRT-PCR Western blot assay performed detect transcription protein levels genes, respectively. Then, functional experiments, including MTT assay, flow cytometry, transwell employed. Besides, interaction between or tripartite motif‑containing 44 (TRIM44) predicted by starbase...
Long noncoding RNAs (LncRNAs) have been shown to play critical roles in the development of diabetic retinopathy (DR), which is often regarded as most frequent cause visual loss world. This study investigated effect and mechanism lncRNA taurine-upregulated gene 1 (TUG1) DR. Quantitative real-time polymerase chain reaction (qRT-PCR) revealed that TUG1 was upregulated streptozotocin (STZ)-induced rat model DR human retinal microvascular endothelial cells (hRMECs) incubated with high glucose...