A5110574889- Ubiquitin and proteasome pathways
- interferon and immune responses
- Histone Deacetylase Inhibitors Research
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- RNA modifications and cancer
- S100 Proteins and Annexins
- TGF-β signaling in diseases
- MicroRNA in disease regulation
- Mesenchymal stem cell research
- Protein Kinase Regulation and GTPase Signaling
- Cancer-related molecular mechanisms research
- Membrane Separation Technologies
- Bone Tissue Engineering Materials
- Bone Metabolism and Diseases
- Peptidase Inhibition and Analysis
- Phytochemical compounds biological activities
- Connective Tissue Growth Factor Research
- Metallic Glasses and Amorphous Alloys
- Cholesterol and Lipid Metabolism
- Traditional and Medicinal Uses of Annonaceae
- Blood properties and coagulation
- GDF15 and Related Biomarkers
- Sphingolipid Metabolism and Signaling
- Nosocomial Infections in ICU
Sichuan University
2016-2024
Dalian Medical University
2024
Second Affiliated Hospital of Dalian Medical University
2024
Martin Luther University Halle-Wittenberg
2024
State Key Laboratory of Oral Diseases
2016-2022
First Affiliated Hospital Zhejiang University
2022
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2022
National Clinical Research Center for Digestive Diseases
2022
Xuzhou Medical College
2019-2020
Chinese Academy of Medical Sciences & Peking Union Medical College
2020
N6-methyladenosine (m6A) is the most abundant epigenetic modification in eukaryotic mRNAs and essential for multiple RNA processing events during mammalian development disease control. Here we show that conditional knockout of m6A methyltransferase Mettl3 bone marrow mesenchymal stem cells (MSCs) induces pathological features osteoporosis mice. loss-of-function results impaired formation, incompetent osteogenic differentiation potential increased adiposity. Moreover, overexpression MSCs...
Abstract Age-related osteoporosis is characterized by the deterioration in bone volume and strength, partly due to dysfunction of marrow mesenchymal stromal/stem cells (MSCs) during aging. Alpha-ketoglutarate (αKG) an essential intermediate tricarboxylic acid (TCA) cycle. Studies have revealed that αKG extends lifespan worms maintains pluripotency embryonic stem (ESCs). Here, we show administration increases mass aged mice, attenuates age-related loss, accelerates regeneration rodents....
Abstract Alveolar bone is the thickened ridge of jaw that supports teeth. It subject to constant occlusal force and pathogens invasion, therefore under active remodeling immunomodulation. holds a distinct niche from long considering their different developmental origin postnatal pattern. However, systematic explanation alveolar at single-cell level still lacking. Here, we construct atlas mouse mandibular through RNA sequencing (scRNA-seq). A more immune microenvironment identified in bone,...
Abstract Objectives Growth differentiation factor 11 (GDF11), an emerging secreted member of the TGF‐beta superfamily, plays essential roles in development, physiology and multiple diseases; however, its role during adipogenic underlying mechanisms remains poorly understood. Materials methods Bone marrow‐derived human mesenchymal stem cells (hMSCs) 3T3‐L1 pre‐adipocytes were induced with culture medium supplementing different concentrations recombinant GDF11 (rGDF11 0, 10, 50, 100 ng mL −1...
Facile fabrication of novel functional membranes with excellent dual thermo- and pH-responsive characteristics has been achieved by simply designing dual-layer composite membranes. pH-Responsive poly(styrene)-block-poly(4-vinylpyridine) (PS-b-P4VP) block copolymers polystyrene blended thermoresponsive poly(N-isopropylacrylamide) (PNIPAM) nanogels are respectively used to construct the top layer bottom The stretching/coiling conformation changes P4VP chains around pKa (∼3.5–4.5) provide...
ABSTRACT N6-methyladenosine (m6A), as a eukaryotic mRNA modification catalyzed by methyltransferase METTL3, is involved in various processes of development or diseases via regulating RNA metabolism. However, the effect METTL3-mediated m6A tooth has remained elusive. Here we show that METTL3 prevalently expressed odontoblasts, dental pulp cells, follicle and epithelial cells Hertwig's root sheath during formation. Depletion human (hDPCs) impairs proliferation, migration, odontogenic...
Prospero-related homeobox protein (Prox1) plays essential roles in the development of many tissues and organs. In present study, we show that Prox1 is modified by small ubiquitin-like SUMO-1 cultured cells. Mutation analysis identified at least four potential sumoylation sites within repression domain Prox1. Our data indicate reduces its interaction with HDAC3 as a result downregulates corepressor activity. These findings suggest may serve novel mechanism for regulation Prox1's
Immunosuppressive agents have been recognized as a factor affecting bone metabolism. The aim of the present study was to evaluate influence FK ‐506 administration on healing around titanium implants. Thirty‐two male mice were randomly allocated two groups: group ( n = 16 mice), which received subcutaneous (1 mg kg −1 d ); and control saline solution by same route administration. After 2 wk, one implant with sandblasted/acid‐etched surface placed in femur. therapy continued until killed 4 wk...
AF4/FMR2 family member 1 (AFF1), known as a central scaffolding protein of super elongation complex (SEC), regulates gene transcription. We previously reported that AFF1 inhibited osteogenic differentiation human mesenchymal stromal/stem cells (hMSCs). However, its role in adipogenic has not been elucidated.hMSCs and 3T3-L1 pre-adipocytes were cultured induced for differentiation. Small interfering RNAs (siRNAs) applied to deplete while lentiviruses expressing HA-Aff1 used overexpression....
Activation of cell surface receptors transduces extracellular signals into cellular responses such as proliferation, differentiation and survival. However, important the activation these is their appropriate spatial temporal down-regulation for normal development tissue homeostasis. The Cbl family E3-ubiquitin ligases plays a major role ligand-dependent inactivation receptor tyrosine kinases (RTKs), most notably Epidermal Growth Factor Receptor (EGFR) through ubiquitin-mediated endocytosis...
Cross-talk between G protein-coupled receptors and protein tyrosine kinases is well established, but the phenotypic consequences of these signaling interactions are not completely understood. To investigate role Src family in mitogenic by receptors, we used genetic pharmacological inhibition to study cell growth response endothelin-1. We found that dominant-negative COOH-terminal kinase blocked mesangial endothelin-1, whereas induced v-Ras was unaffected. Endothelin-1-induced antagonist...
Abstract Growth differentiation factor 11 (GDF11), a secreted member of the transforming growth factor‐β (TGF‐β) superfamily, has been reported to have capacity reverse age‐related pathologic changes and regulate organ regeneration after injury; however, role GDF11 in fracture healing bone repair is still unclear. Here, we established model 12‐week‐old male mice observe two states: cartilaginous callus bony formation phases. Our results showed that recombinant (rGDF11) injection inhibits...
Abstract The ubiquitin‐proteasome system is the primary cellular pathway for protein degradation, mediating 80% of intracellular degradation. Because widespread presence ubiquitin‐modified substrates, ubiquitination can regulate a variety activities including cell proliferation, apoptosis, autophagy, endocytosis, DNA damage repair, and immune responses. With continuous generation genomics data in recent years it has become particularly important to analyze these effectively reasonably....