A5091279494- RNA modifications and cancer
- Cancer-related gene regulation
- Cancer-related molecular mechanisms research
- HVDC Systems and Fault Protection
- Cerebrovascular and Carotid Artery Diseases
- Ectopic Pregnancy Diagnosis and Management
- Gestational Trophoblastic Disease Studies
- RNA and protein synthesis mechanisms
- Cardiovascular Health and Disease Prevention
- RNA Research and Splicing
- Pelvic and Acetabular Injuries
- Radioactive element chemistry and processing
- Ovarian cancer diagnosis and treatment
- Cardiovascular Function and Risk Factors
- Renal and Vascular Pathologies
- Organ Transplantation Techniques and Outcomes
- Radioactive contamination and transfer
- Vector-borne infectious diseases
- Viral Infections and Vectors
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Pancreatic and Hepatic Oncology Research
- Maternal and Perinatal Health Interventions
- Immune cells in cancer
- Protein Hydrolysis and Bioactive Peptides
- Pelvic floor disorders treatments
National Clinical Research Center for Digestive Diseases
2024
First Affiliated Hospital Zhejiang University
2024
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2024
The First Affiliated Hospital, Sun Yat-sen University
2018-2024
Sun Yat-sen University
2018-2024
Huazhong Agricultural University
2019-2024
Shanghai Ninth People's Hospital
2021-2023
Shanghai Jiao Tong University
2021-2023
Harbin Medical University
2022
Second Affiliated Hospital of Harbin Medical University
2022
N6-methyladenosine (m6A) is the most abundant epigenetic modification in eukaryotic mRNAs and essential for multiple RNA processing events during mammalian development disease control. Here we show that conditional knockout of m6A methyltransferase Mettl3 bone marrow mesenchymal stem cells (MSCs) induces pathological features osteoporosis mice. loss-of-function results impaired formation, incompetent osteogenic differentiation potential increased adiposity. Moreover, overexpression MSCs...
Abstract Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal human cancers. It thrives in a malnourished environment; however, little known about mechanisms by which PDAC cells actively promote aerobic glycolysis to maintain their metabolic needs. Gene Expression Omnibus (GEO) was used identify differentially expressed miRNAs. The expression pattern miR-30d normal and tissues studied situ hybridization. role miR-30d/RUNX1 vitro vivo evaluated CCK8 assay clonogenic formation as...
Abstract Mis-regulated RNA modifications promote the processing and translation of oncogenic mRNAs to facilitate cancer progression, while molecular mechanisms remain unclear. Here we reveal that tRNA m 7 G methyltransferase complex proteins METTL1 WDR4 are significantly up-regulated in esophageal squamous cell carcinoma (ESCC) tissues associated with poor ESCC prognosis. In addition, progression via activity vitro vivo. Mechanistically, or knockdown leads decreased expression G-modified...
The frequency of p53 mutations in colorectal cancer (CRC) is approximately 40-50%. A variety therapies are being developed to target tumors expressing mutant p53. However, potential therapeutic targets for CRC wild-type rare. In this study, we show that METTL14 transcriptionally activated by and suppresses tumor growth only p53-wild-type (p53-WT) cells. deletion promotes both AOM/DSS AOM-induced mouse models with the intestinal epithelial cell-specific knockout METTL14. Additionally,...
Esophageal cancer is a lethal malignancy with high mortality rate, while the molecular mechanisms underlying esophageal pathogenesis are still poorly understood. Here, we found that N6-methyladenosine (m<sup>6</sup>A) methyltransferase-like 3 (METTL3) significantly upregulated in squamous cell carcinoma (ESCC) and associated poor patient prognosis. Depletion of METTL3 results decreased ESCC growth progression <i>in vitro</i> vivo</i>. We further established initiation models using...
The Pax7+ muscle stem cells (MuSCs) are essential for skeletal homeostasis and regeneration upon injury, while the molecular mechanisms underlying cell fate determination still not fully understood. N6-methyladenosine (m6A) RNA modification is catalyzed by METTL3 plays important functions in posttranscriptional gene expression regulation various biological processes. Here, we generated cell-specific conditional knockout mouse model revealed that significantly inhibits proliferation of blocks...
Background CD276 (B7-H3), a pivotal immune checkpoint, facilitates tumorigenicity, invasiveness, and metastasis by escaping surveillance in variety of tumors; however, the underlying mechanisms facilitating escape esophageal squamous cell carcinoma (ESCC) remain enigmatic. Methods We investigated expression ESCC tissues from patients using immunohistochemistry (IHC) assays. In vivo, we established 4-nitroquinoline 1-oxide (4NQO)-induced knockout (CD276 wKO ) K14cre; conditional cKO mouse...
Ribosome RNA (rRNA) accounts for more than 80% of the cell's total RNA, while physiological functions rRNA modifications are poorly understood. Mutations 18S m6A methyltransferase METTL5 cause intellectual disability, microcephaly, and facial dysmorphisms in patients, however, little is known about underlying mechanisms. In this study, we identified protein complex revealed that mainly interacts with binding proteins ribosome proteins. Functionally, found Mettl5 knockout mESCs leads to...
The aim of this study was to obtain egg-derived peptides with facilitating alcohol metabolism (EPs) by enzymolysis, identify their structures, and screen small polypeptides higher activity molecular docking. optimum conditions for preparing EPs were obtained a single factor experiment, adding 2% Protamex performing enzymolysis 3 h liquid–material ratio 35:1. dose–response relationship experiment showed that 800 mg/kg·bw played better role in metabolism. contained 40% hydrophobic amino acids...
To evaluate the feasibility of ultrafast ultrasound pulsed wave velocity (PWV) for carotid stiffness assessment and potential influencing factors.Ultrafast PWV measurements 442 arteries in 162 consecutive patients (patient group) 66 healthy volunteers (control were performed. High- very high-frequency transducers used 110 segments. The PWVs at beginning end systole automatically measured. correlations between intima-media thickness (IMT) equipment factors utility analyzed.Each acquisition...
Abstract Although the function of tRNAs in translational process is well established, it remains controversial whether tRNA abundance tightly associated with efficiency (TE) mammals. Moreover, how critically expression contributes to establishment tissue-specific proteomes mammals has not been addressed. Here, we measured both using demethylase-tRNA sequencing (DM-tRNA-seq) and TE mRNAs ribosome-tagging (RiboTag-seq) brain, heart, testis mice. Remarkable variation isodecoders was observed...
Inositol 1,4,5-Triphosphate Receptor Family (ITPRs) are necessary intracellular Ca2+-release channel encoders and participate in mammalian cell physiological pathological processes. Previous studies have suggested that ITPRs tumorigenesis of multiple cancers. Nevertheless, the diverse expression profiles prognostic significance three pancreatic cancer yet to be uncovered. In this work, we examined levels survival dates patients with cancer. As a result, identified ITPR1 ITPR3 significantly...
Abstract Although the function of tRNA in translational process is well established, it remains controversial whether abundance tightly associated with efficiency (TE) mammals. For example, how critically expression tRNAs contributes to establishment tissue-specific proteomes mammals has not been addressed. Here, we measured both using DM-tRNA-seq and ribosome-associated mRNAs brain, heart, testis RiboTag mice. Remarkable variation isodecoders was observed among different tissues. When...
Background: Hepatocellular carcinoma (HCC) is a common primary malignant tumor and cause of cancer-related death in humans. Increasing evidence indicates that an imbalance N6-methyladenosine (m6A) methylation strongly linked to the occurrence development cancer. We used comprehensive bioinformatics establish potential prognostic model HCC based on m6A methylation-related genes. And case analyses were verify results. Methods: The clinical data gene expressions obtained from Cancer Genome...
Piroplasmosis is a disease of domestic and wild animals caused by tick-borne protozoa the genera Theileria Babesia. leads to substantial economic losses in livestock industry. This has been frequently reported subtropical tropical regions worldwide. However, information regarding prevalence piroplasma ticks collected from dogs cattle lacking most areas. To assess potential threat piroplasmosis South China, 671 were Guangxi Province. Deoxyribonucleic acid (DNA) was extracted these evaluate...
Abstract Background: Ectopic pregnancy can be life-threatening. Most of ectopic located in the fallopian tube. But retroperitoneal pregnancies are extremely rare.Results: We report a 29-year-old Chinese woman with amenorrhea for 50 days presented to emergency department abdominal pain. Her serum β-hCG level was 1675mIU/ml. Transvaginal sonography showed irregular anechoic area uterine cavity without sac echo. And nothing wrong found bilateral adnexa. Abdominal ultrasound mixed echo region...