A5101857487- Gut microbiota and health
- MicroRNA in disease regulation
- Cancer, Hypoxia, and Metabolism
- Gastric Cancer Management and Outcomes
- Advanced biosensing and bioanalysis techniques
- RNA modifications and cancer
- Cancer-related molecular mechanisms research
- Epigenetics and DNA Methylation
- Helicobacter pylori-related gastroenterology studies
- Liver Disease Diagnosis and Treatment
- Cancer-related gene regulation
- Mesenchymal stem cell research
- Liver Disease and Transplantation
- Pancreatic function and diabetes
- Cytokine Signaling Pathways and Interactions
- Monoclonal and Polyclonal Antibodies Research
- Autophagy in Disease and Therapy
- Chemokine receptors and signaling
- Genomics, phytochemicals, and oxidative stress
- Metal complexes synthesis and properties
- Probiotics and Fermented Foods
- Cancer Research and Treatments
- Esophageal Cancer Research and Treatment
- Colorectal Cancer Surgical Treatments
- Luminescence and Fluorescent Materials
Shanghai Ninth People's Hospital
2016-2024
Shanghai Jiao Tong University
2013-2024
Affiliated Hospital of Qingdao University
2024
Shanghai Veterinary Research Institute
2022-2024
Qingdao University
2024
Shanghai Sixth People's Hospital
2016
Third Affiliated Hospital of Sun Yat-sen University
2008-2013
Sun Yat-sen University
2008-2013
Shanghai First People's Hospital
2011-2013
Université de Strasbourg
2009-2012
Abstract Pancreatic ductal adenocarcinoma (PDAC) is one of the most lethal human cancers. It thrives in a malnourished environment; however, little known about mechanisms by which PDAC cells actively promote aerobic glycolysis to maintain their metabolic needs. Gene Expression Omnibus (GEO) was used identify differentially expressed miRNAs. The expression pattern miR-30d normal and tissues studied situ hybridization. role miR-30d/RUNX1 vitro vivo evaluated CCK8 assay clonogenic formation as...
Abstract Cisplatin-derived anticancer therapy has been used for three decades despite its side effects. Other types of organometallic complexes, namely, some ruthenium-derived compounds (RDC), which would display cytotoxicity through different modes action, might represent alternative therapeutic agents. We have studied both in vitro and vivo the biological properties RDC11, one most active a new class RDCs that contain covalent bond between ruthenium atom carbon. showed RDC11 inhibited...
Accumulating evidence suggests that the insect and mammalian innate immune response is mediated by homologous regulatory components.Proinflammatory cytokines bacterial lipopolysaccharide stimulate immunity activating transcription factors such as NF-B AP-1.One of responses evoked these stimuli initiation a kinase cascade leads to phosphorylation p38 mitogen-activated protein (MAP) on Thr Tyr within motif Thr-Gly-Tyr, which located subdomain VIII.We have investigated possible involvement MAP...
Developing non-cationic gene carriers and achieving efficient endo/lysosome escape of functional nucleic acids in cytosol are two major challenges faced by the field delivery. Herein, we demonstrate concept self-escape spherical acid (SNA) to achieve light controlled delivery with sufficient capacity. In this system, Bcl-2 antisense oligonucleotides (OSAs) were conjugated onto surface aggregation-induced emission (AIE) photosensitizer (PS) nanoparticles form core-shell SNA. Once SNAs taken...
Abstract The development of chemoresistance remains a major challenge that accounts for colorectal cancer (CRC) lethality. Dichloroacetate (DCA) was originally used as metabolic regulator in the treatment diseases; here, DCA assayed to identify mechanisms underlying CRC. We found markedly enhanced chemosensitivity CRC cells fluorouracil (5-FU), and reduced colony formation due high levels apoptosis. Using microarray assay, we noted miR-149-3p involved CRC, which modulated by wild-type p53...
The frequency of p53 mutations in colorectal cancer (CRC) is approximately 40-50%. A variety therapies are being developed to target tumors expressing mutant p53. However, potential therapeutic targets for CRC wild-type rare. In this study, we show that METTL14 transcriptionally activated by and suppresses tumor growth only p53-wild-type (p53-WT) cells. deletion promotes both AOM/DSS AOM-induced mouse models with the intestinal epithelial cell-specific knockout METTL14. Additionally,...
Breast cancer (BrCa) is the malignant tumor that most seriously threatens female health; however, molecular mechanism underlying its progression remains unclear. Here, we found conditional deletion of hypermethylated in 1 (HIC1) mouse mammary gland might contribute to premalignant transformation early stage formation. Moreover, chemokine (C-X-C motif) ligand 14 (CXCL14) secreted by HIC1-deleted BrCa cells bound cognate receptor GPR85 on fibroblasts microenvironment and was responsible for...
Liver fibrosis is a key phase that will progress to further injuries such as liver cirrhosis or carcinoma. This study aimed investigate whether transplantation of bone marrow mesenchymal stromal cells (BM-MSCs) can attenuate in mice and the underlying mechanisms based on regulation macrophage subtypes.A model was induced by intraperitoneal (i.p.) injection CCl4 twice per week for 70 days, BM-MSCs were intravenously transplanted 60th 70th days. Immunohistology gene expression subtypes...
Abstract Exploring novel anticancer drugs to optimize the efficacy may provide a benefit for treatment of colorectal cancer (CRC). Disulfiram (DSF), as an antialcoholism drug, is metabolized into diethyldithiocarbamate-copper complex (CuET) in vivo, which has been reported exert effects on various tumors preclinical studies. However, little known about whether CuET plays anti-cancer role CRC. In this study, we found that had marked effect suppressing CRC progression both vitro and vivo by...
Chemoresistance is responsible for most colorectal cancer (CRC) related deaths. In this study, we found that dichloroacetate (DCA), a pyruvate dehydrogenase kinase (PDK) inhibitor, can be used as sensitizer oxaliplatin (L-OHP) chemoresistant CRC cells. The aim of study was to explore the ability DCA overcome L-OHP resistance in cells and identify underlying molecular mechanisms. We sensitizes L-OHP-induced cytotoxic effects by inhibiting clone formation capacity promoting cell apoptosis. A...
Abstract Background Chemoresistance remains a critical event that accounts for colorectal cancer (CRC) lethality. The aim of this study is to explore the ability dichloroacetate (DCA) increase chemosensitivity in CRC and molecular mechanisms involved. Methods effects combination treatment DCA oxaliplatin (L-OHP) were analysed both vitro vivo. DCA-responsive proteins AMPK pathway enriched using proteomic profiling technology. effect on CAB39–AMPK signal was analysed. In addition, miRNA...
KRASG12C inhibitors (KRASG12Ci) AMG510 and MRTX849 have shown promising efficacy in clinical trials been approved for the treatment of KRASG12C-mutant cancers. However, emergence therapy-related drug resistance limits their long-term potential. This study aimed to identify critical mediators develop overcoming strategies.By using RNA sequencing, RT-qPCR immunoblotting, we identified validated upregulation c-Myc activity amplification long noncoding ST8SIA6-AS1 KRASG12Ci-resistant cells. The...
Protein lysine acetylation and succinylation play important regulatory roles in cells, both of which or each other has a close relationship. Dichloroacetate (DCA), well-known pyruvate dehydrogenase kinase (PDK) inhibitor, the potential to be used as anti-cancer drugs for several tumors including colorectal cancer. However, little is known about mechanism DCA-based cancer therapy by protein posttranslational modifications (PTM) global proteome, acetylome succinylome. Here combinations with...
New-onset diabetes mellitus has a rough correlation with pancreatic cancer (PaC), but the underlying mechanism remains unclear.This study aimed to explore exosomal microRNAs and their potential role in PaC-induced β-cell dysfunction.The β cells were treated isolated exosomes from PaC cell lines, SW1990 BxPC-3, before measuring glucose-stimulated insulin secretion (GSIS), validating that BxPC-3 might disrupt GSIS of both line MIN6 primary mouse islets.The difference expression profiles...
ABSTRACT The initiation of mesoderm differentiation in the Drosophila embryo requires gene products twist and snail. In either mutant, ventral cell invagination during gastrulation is blocked no mesoderm-derived tissue formed. One functions Snail to repress neuroectodermal genes restrict their expressions lateral regions. derepression into region snail mutant a possible cause defects differentiation. To investigate such possibility, we analysed series alleles. We found that different respond...
Background & Aims. Severe acute pancreatitis (SAP) remains a high-mortality disease. Bone marrow (BM) mesenchymal stem cells (MSCs) have been demonstrated to plasticity of transdifferentiation and immunomodulatory functions. In the present study, we assessed roles MSCs in SAP therapeutic effects MSC on after transplantation. Methods. A rat model was induced by injection taurocholic acid (TCA) into pancreatic duct. After isolation characterization from BM, transplantation conducted 24 hrs...
WHSC1 is a histone methyltransferase that facilitates H3 lysine 36 dimethylation (H3K36me2), which permissive mark associated with active transcription. In this study, we revealed how regulates tumorigenesis and chemosensitivity of colorectal cancer (CRC). Our data showed as well H3K36me2 were highly expressed in clinical CRC samples, high expression poorer prognosis OS patients. reduction promoted colon cell apoptosis both vivo vitro. We found B lymphoma-2 (BCL2) expression, an...