To investigate the impacts of uterine type and conceptus genotype on development through late gestation, Meishan Yorkshire embryos were co-transferred into uteri either or recipients that subsequently slaughtered Day 90 gestation. At slaughter, regardless genotype, fetuses placentae markedly smaller when recovered from than recipients. Whereas similar in weight, lighter littermate uteri. Because marked differences between fetal weights observed to allowed farrow. Surprisingly, cogestated...

Introduction Numerous factors are known to influence reproductive efficiency in ewes, but few studies have investigated the potential role of vaginal microbiota sheep success. The objective this study was thoroughly characterize ewe throughout course pregnancy. Methods Vaginal samples were collected from 31 pregnant Hampshire and X Suffolk crossbred ewes on a weekly basis pre-breeding pregnancy testing then biweekly until just after lambing. To microbial communities, DNA extracted 16S rRNA...

Investigating the livestock vaginal microbiota is of increasing interest due to its relationship with animal reproductive performance. Recent publications have uncovered a high degree variability microbiota, making it difficult focus functional research on individual microorganisms. To address this variability, we conducted combined analysis publicly available 16S rRNA gene amplicon sequencing datasets reveal core in cattle, sheep, and pigs. The goal was identify bacterial genera that were...

Little is known regarding the vaginal microbiota of sheep that undergo spontaneous abortions. The aim this pilot study was to characterize, using 16S rRNA gene sequencing and shotgun metagenomics, throughout gestation two ewes (Ewe1 Ewe2) spontaneously aborted. Ewe1’s contained high abundances Histophilus, Staphylococcus, Unclassified Pasteurellaceae. Most notable abundance Campylobacter in following abortion. Ewe2’s characterized by Pasteurella gestation. Shotgun metagenomic produced...

Query (Q) fever is a globally distributed zoonotic disease caused by Coxiella burnetii, bacterial agent for which ruminants are the most prevalent natural reservoir. Data regarding Q infection in camels Algeria limited. Therefore, survey to detect seroprevalence of C. burnetii antibodies was conducted among healthy camel populations vast area southeastern determine distribution causative organism and identify risk factors associated with infection. Between January March 2016, blood samples...

Three experiments were conducted to examine the in vitro development of preimplantation embryos from prolific Chinese Meishan pig. Experiment 1 was assess whether would develop and retain their viability, whereas Exp. 2 3 examined developmental pattern embryos. In all three experiments, Yorkshire served as a contemporary comparison. Ovulation embryo recovery rates not different between gilts. cultured for 96 h capable establishing pregnancies. The number cell nuclei present after 144 culture...

The objectives of this research were to determine the effects β-mercaptoethanol (β-ME) and fetal bovine serum (FBS) on in vitro development embryos derived from oocytes matured fertilized examine mechanism through which β-ME may influence embryo development. A 2 × factorial treatment arrangement was used evaluate effect 0 or 100 µM 0% 10% FBS cultured Medium 199 (M199) absence somatic cells. Embryos randomly allocated within stage (< 8 cells 8–16 cells) one four combinations for 6 days. Both...

A reciprocal embryo transfer study with Meishan and Yorkshire pigs was conducted to examine the influence of embryonic genotype uterine environment on preimplantation growth development. Embryos were collected from gilts d 2 a spontaneous estrous cycle (d 0 = onset estrus), transferred synchronous recipients, recipients 12 obtain measurements diameter (size) DNA, protein, estrogen content. No difference detected between donors for number ovulations, embryos recovered, or cells per embryo....

SUMMARYSheep are a valuable livestock species because of their ability to convert forages, as well feedstuffs not suitable for human consumption, into meat and milk that important sources dietary protein. Sheep the most abundant ruminant in Egypt, great opportunity exists enhance productivity though implementation genetic improvement program utilizing advanced reproductive technologies artificial insemination embryo transfer. These two permit production more offspring from genetically...

Reproductive performance is paramount to the success of livestock production enterprises focused on lamb meat production. influenced by various factors, possibly including reproductive tract microbial communities present at time copulation and throughout pregnancy. There are few publications that identify vaginal livestock, even fewer exist for sheep. To compare ewe communities, swabs were taken from 67 Hampshire X Suffolk crossbred ewes Iowa State University sheep farm a pre-breeding point...

The “Dairy Village” concept was designed to overcome the constraints of estrus synchronization and AI particularly associated with eletion breeding animals subsequent management estrous cows in Ethiopia. All necessary inputs, including sexed semen, were acquired before fieldwork. knowledge skill gaps identified addressed active involvement veterinarians technicians who underwent specialized training ensure proper physical gynecological examinations during selection heifers. Accordingly, a...

Day 12 blastocysts from Meishan gilts contain fewer cells than do day Yorkshire gilts. The purpose of this study was to evaluate the effect breed on relative numbers inner cell mass and trophectoderm in embryos at similar stages. Embryos were collected days 5.5–6.5 gestation subjected image analysis differential staining. No differences detected thickness zona pellucida or areas perivitelline space, embryo proper, blastocoel any developmental stages examined (compact morula, early blastocyst...

Preimplantation embryos from cattle, sheep, and goats may be cryopreserved for short- or long-term storage. consist predominantly of water, the avoidance intracellular ice crystal formation during cryopreservation process is paramount importance to maintain embryo viability. Embryos are placed into a hypertonic solution (1.4 – 1.5 M) cryoprotective agent (CPA) such as ethylene glycol (EG) glycerol (GLYC) create an osmotic gradient that facilitates cellular dehydration. After reach...

The objective of this study was to evaluate the impact different management systems on postnatal survival and growth alpaca crias. conducted during calving season in Peruvian Andes. Animals were fed native pastures; day they went out graze, but at night brought into a corral. A total 150 singleton neonates randomly assigned one three cria protection strategies immediately after consuming colostrum. first group consisted 50 crias who slept an open-corral (OC) without shelter. second comprised...

The current study aimed to explore the potential usefulness of liquid or lyophilized egg yolk plasma (EYP) as a substitute for low-density lipoproteins (LDL) cryopreservation canine spermatozoa. In first experiment, total 20 ejaculates harvested from six Beagles were frozen in extenders containing 6% LDL (control) EYP at one three concentrations (20%, 40% 60%). Motility parameters assessed 10 min after thawing using computer-assisted sperm analysis. For both and EYP, concentration yielded...

Surra, caused by Trypanosoma evansi , is a re-emerging animal trypanosomosis, which of special concern for camel-rearing regions Africa and Asia. Surra decreases milk yield, lessens body condition score reduces market value exported animals resulting in substantial economic losses. A cross-sectional seroprevalence study dromedary camels was conducted Algeria, major risk factors associated with infection were identified collecting data on characteristics herd management practices. The T....

Abstract The objective was to evaluate the effects of peripartum supplementation a methionine hydroxy analogue (MHA) primiparous, spring-calving beef females on dam and progeny performance. Angus heifers (n = 60) were blocked by expected parturition date, stratified body weight (BW) condition score (BCS), randomized 1 15 pens. Pens randomly assigned 3 dietary treatments: basal diet supplemented with 0 (M0), (M15), or 30 (M30) g/animal/d MHA (provided as MFP feed supplement, Novus...

The effect of sex on pig conceptus development to day 12 gestation was investigated.On 2 gestation, reciprocal embryo transfers were performed resulting in four groups (Yorkshire\p=n-\Yorkshire,Yorkshire\p=n-\Meishan,Meishan\p=n-\Yorkshireand Meishan\p=n-\Meishan).Conceptuses at recovered from each recipient and diameter, as well DNA, protein oestradiol content determined for individual conceptuses.The conceptuses by amplification a fragment the SRY gene, using polymerase chain...