A5070457912- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Animal Genetics and Reproduction
- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Veterinary Medicine and Surgery
- Extracellular vesicles in disease
- Urologic and reproductive health conditions
- Ovarian function and disorders
- Reproductive Health and Technologies
- Veterinary Oncology Research
- Intestinal Malrotation and Obstruction Disorders
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- Xenotransplantation and immune response
- Renal and related cancers
- Neuroendocrine regulation and behavior
- Urological Disorders and Treatments
- Veterinary Equine Medical Research
- Veterinary Pharmacology and Anesthesia
- Urinary Bladder and Prostate Research
- Animal Behavior and Welfare Studies
- Bone Metabolism and Diseases
- Esophageal and GI Pathology
Universidad de Murcia
2014-2025
Instituto Murciano de Investigación Biosanitaria
2017-2022
Institut de la Vision
2020
Linköping University
2019
Abstract Background Porcine seminal plasma (SP) is endowed with a heterogeneous population of extracellular vesicles (sEVs). This study evaluated the immunophenotypic profile by high-sensitivity flow cytometry eight sEV subpopulations isolated according to their size (small [S-sEVs] and large [L-sEVs]) from four different SP sources, namely three ejaculate fractions (the first 10 mL sperm rich fraction [SRF-P1], remaining SRF [SRF-P2], post-SRF [PSRF]) entire (EE). Methods Seminal EVs were...
A fibreoptic endoscope procedure for non-surgical deep intrauterine insemination in non-sedated sows has been reported. However, the is an expensive and fragile instrument, unsuitable use under field conditions. The aim of this study was to determine minimum number spermatozoa required maintain optimal fertility using a flexible catheter (1.8 m length, 4 mm diameter) 2-6 parity sows. Crossbred were treated with eCG 24 h after weaning hCG 72 later induce oestrus. Deep performed 36 treatment...
A 100-fold reduction of the standard dose for artificial insemination in pigs (3 x 10(9) spermatozoa 80-100 ml fluid) can be used when are deposited surgically next to uterotubal junction. The present study was performed develop a technique non-surgical deep intrauterine without sedation animal. In Expt 1, sows were weaned, treated induce oestrus and evaluate difficulties involved insertion flexible fibre optic endoscope through cervix along uterine horn. Deep catheterizations on each sow at...
Seminal plasma contains a heterogeneous population of extracellular vesicles (sEVs) that remains poorly characterized. This study aimed to characterize the lipidomic profile two subsets differently sized sEVs, small (S-) and large (L-), isolated from porcine seminal by size-exclusion chromatography characterized an orthogonal approach. High-performance liquid chromatography-high-resolution mass spectrometry was used for analysis. A total 157 lipid species 14 classes 4 major categories...
Abstract Background Oxidative stress, a source of genotoxic damage, is often the underlying mechanism in many functional cell disorders. Extracellular vesicles (EVs) have been shown to be key regulators cellular processes and may involved maintaining redox balance. Herein, we aimed develop method assess effects EVs on DNA oxidation using porcine seminal plasma extracellular (sEVs). Results The technique was set cell-free plasmid avoid bias generated by uptake sEVs sperm cells, employing...
The present study was designed to evaluate the brilliant cresyl blue (BCB) test as a means of selection immature pig oocytes improve performance homologous in vitro penetration (hIVP) assay carried out with assess penetrating ability boar spermatozoa. Immature harvested from abattoir ovaries were incubated 13 µМ BCB phosphate-buffered saline, and selected according their colour (blue colourless oocytes) after 90 min incubation at 39°C 5% CO 2 air. To criteria (Experiment 1), diameter...
Contents This study was performed to characterize the normal blood flow of canine testis and measure peak systolic velocity (PSV), end‐diastolic (EDV), resistive index (RI) pulsatility (PI) testicular arteries weekly during a period 6 months in five healthy Beagle dogs evaluate whether there were any change along this time. The ultrasonographic exams made with an 11 MHz linear transducer. vessels testes subdivided into three categories: supratesticular arteries, marginal artery...
Background: Seminal plasma (SP) promotes sperm survival and fertilizing capacity, potentially affects embryo development, presumably via specific signaling pathways to the internal female genital tract. Objectives: This study evaluated how heterologous SP, infused immediately before postcervical artificial insemination (AI) affected development transcriptional pattern of pig endometria containing embryos. Materials Methods: Postweaning estrus sows (n = 34) received 40-mL intrauterine...
Abstract The coenzyme Q10 (CoQ10) is a potent antioxidant with critical protection role against cell oxidative stress, caused by the mitochondrial dysfunction. This study evaluated effects of CoQ10 supplementation to in vitro maturation (IVM) or embryo culture media on maturation, fertilization and subsequent embryonic development pig oocytes embryos. Maturation (Experiment 1) 2) were supplemented 0 (control), 10, 25, 50 100 μM CoQ10. addition 10–50 IVM medium did not affect percentage MII...
Introduction Pig seminal plasma (SP) is rich in active forms of all three isoforms (1-3) transforming growth factor β (TGF-β), a chemokine modulatory the immune environment female genital tract once semen delivered during mating or artificial insemination (AI). The present study aimed to examine how TGF-βs are secreted by epithelium male reproductive and they transported semen, emphasizing interplay with extracellular vesicles (sEVs). Methods Source was examined immunohistochemistry testis,...
The seminal plasma (SP) modulates the female reproductive immune environment after mating, and microRNAs (miRNAs) could participate in process. Considering that boar ejaculate is built by fractions differing SP-composition, this study evaluated whether exposure of mucosal explants sow internal genital tract (uterus, utero-tubal junction isthmus) to different SP-fractions changed profile explant-secreted miRNAs. Mucosal retrieved from oestrus sows (n = 3) were vitro exposed to: Medium 199...