A5055970940- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Sperm and Testicular Function
- CRISPR and Genetic Engineering
- Neuroendocrine regulation and behavior
- Renal and related cancers
- Reproductive Health and Technologies
- MicroRNA in disease regulation
- Animal Behavior and Welfare Studies
- Coenzyme Q10 studies and effects
- Virus-based gene therapy research
- Cystic Fibrosis Research Advances
- Agriculture Sustainability and Environmental Impact
- Animal Nutrition and Physiology
- Cytokine Signaling Pathways and Interactions
- Rabbits: Nutrition, Reproduction, Health
- Animal Nutrition and Health
- Bioenergy crop production and management
- Immune responses and vaccinations
- Animal Virus Infections Studies
- Neonatal Respiratory Health Research
Technical University of Munich
2023-2025
Institute of Bioinformatics and Systems Biology
2025
Instituto Murciano de Investigación Biosanitaria
2019-2024
Universidad de Murcia
2017-2024
Linköping University
2020
The results obtained using precision feeding in maternities (electronic sow feeders, ESFs) may be affected by the type of farrowing pen, as design pen modify behavior sows. Hence, aim present study was to determine effects and possible interactions electronic systems different types pens (traditional, socialization loose housing pens) on productive economic yields lactating hyperprolific sows under commercial farm conditions. showed an overall lower feed disappearance fed with ESFs and,...
In patients with cystic fibrosis (CF), repeated cycles of infection and inflammation eventually lead to fatal lung damage. Although diminished mucus clearance can be restored by highly effective CFTR modulator therapy, often persist. To elucidate the role innate immune system in CF etiology, we investigated a pig model compared these results those for preschool children CF. newborn pigs, observed changes cell composition before onset that were dominated increased monocyte infiltration,...
The use of electronic sow feeders (ESFs) during lactation has been associated with weaning heavier piglets when compared to traditional feeders, a lower amount feed per kg weaned piglet, improved welfare the sow, and no negative effects on body condition or metabolic traits at subsequent reproductive yields. However, there have studies assessing possible ESF lifelong development progeny. This study reveals that from sows fed ESFs were than those (5.91 ± 1.45 vs. 5.58 1.23 kg, p < 0.005),...
Background: Seminal plasma (SP) promotes sperm survival and fertilizing capacity, potentially affects embryo development, presumably via specific signaling pathways to the internal female genital tract. Objectives: This study evaluated how heterologous SP, infused immediately before postcervical artificial insemination (AI) affected development transcriptional pattern of pig endometria containing embryos. Materials Methods: Postweaning estrus sows (n = 34) received 40-mL intrauterine...
The infusion of boar seminal plasma (SP) before artificial insemination (AI) positively alters the expression endometrial genes and pathways involved in embryo development. This study aimed to determine which transcriptome changes occur preimplantation embryos response SP infusions during estrus. Postweaning estrus sows received 40-mL intrauterine either (N = 6) or BTS extender (control group; N 30 min each two post-cervical AIs. On Day 6, were surgically collected analyzed for differential...
Abstract The coenzyme Q10 (CoQ10) is a potent antioxidant with critical protection role against cell oxidative stress, caused by the mitochondrial dysfunction. This study evaluated effects of CoQ10 supplementation to in vitro maturation (IVM) or embryo culture media on maturation, fertilization and subsequent embryonic development pig oocytes embryos. Maturation (Experiment 1) 2) were supplemented 0 (control), 10, 25, 50 100 μM CoQ10. addition 10–50 IVM medium did not affect percentage MII...
This study was designed to investigate the impact of vitrification on transcriptome profile blastocysts using a porcine (Sus scrofa) model and microarray approach. Blastocysts were collected from weaned sows (n = 13). A total 60 vitrified (treatment group). After warming, embryos cultured in vitro for 24 h. Non-vitrified 40) used as controls. culture period, embryo viability morphologically assessed. 30 viable per group (three pools 10 4 different donors each) subjected gene expression...
Cloned and transgenic pigs are relevant human disease models serve as potential donors for regenerative medicine xenotransplantation. These technologies demand oocytes embryos of good quality. However, the current protocols in vitro production (IVP) pig give reduced blastocyst efficiency embryo quality compared to vivo controls. This is likely due culture conditions jeopardizing embryonic homeostasis including effect reactive oxygen species (ROS) influence. In this study, antioxidant...
The Superfine Open Pulled Straw (SOPS) system is the most commonly used method for vitrification of pig embryos. However, this only allows four to seven embryos per straw. In study, we investigated effectiveness open (OC) and closed (CC) Cryotop ® systems simultaneously vitrify a larger number porcine Morulae, early blastocysts full were vitrified with ( n = 250; 20 device) system, 158; traditional superfine pulled straw (SOPS; 241; 4–7 straw) method. Fresh from each developmental stage...
Abstract Background Proteome characterization of the porcine endometrium and extraembryonic membranes is important to understand mother-embryo cross-communication. In this study, proteome chorioallantoic membrane was characterized in pregnant sows (PS) during early gestation (d 18 24 gestation) non-pregnant (NPS) same days using LC-MS/MS analysis. The UniProtKB database ClueGO were used obtain functional Gene Ontology annotations biological networks, respectively. Results Our analysis...
This study examined how the vitrification of pig blastocysts using either superfine open-pulled straw (SOPS) or Cryotop method affects expression profile embryonic microRNA (miRNA) transcriptomes, as well its relation to changes in target genes (TGs). Surgically collected were vitrified SOPS (n = 60; 4-6 embryos/device) system 20 embryos/device). Embryos cultured vitro for 24 h after warming. Fresh 60) hours served controls. After culture, five pools eight viable from each group prepared...
Despite its advantages for pig breeding, embryo transfer (ET) has a major handicap: high mortality during the pre- and implantation period, probably caused by divergent phenomena of tolerance between immunologically unrelated (i.e., allogeneic) embryos recipient sow. Thus, to reach similar maternal as in conventional breeding artificial insemination (AI) would be key ET-success. For this reason, we studied expression leukemia inhibitory factor (LIF) cytokine receptor endometrium period (days...
Although embryo transfer (ET) is a biotechnology ready for the swine industry, there are factors to be solved, availability of donors as one. Multiparous sows ought considered since weaning natural and efficient method estrus synchronization. In addition, superovulation treatments at effective in increasing efficiency donor production. However, ET programs typically require more than those available from single weaning, imposing grouping several weanings establish batch ET. Since short-term...
The most commonly used technique to vitrify pig embryos is the super open pulled straw (SOPS), where a maximum of 6 can be vitrified simultaneously per device without compromising minimum volume necessary for optimal preservation. Since embryo transfer (ET) demands 20-40 recipient, customary use SOPS complicates warming and ET in field conditions. Such complications could avoided when using Cryotop® (OC) system, which has been proven an effective option vitrifying at least 20 porcine...
The combination of estrus synchronization and superovulation (SS) treatments causes alterations in ovarian endometrial gene expression patterns, resulting abnormal follicle oocyte growth, fertilization, embryo development. However, the impact combined SS on transcriptome surviving embryos remains unidentified. In this study, we examined changes day 6 blastocysts that survived a brief regimen treatment with superovulation. sows were included one three groups: SS7 group (n = 6), administered...
Vitrification is the ideal method for long-lasting storage of porcine embryos. However, both strict airline regulations transport liquid nitrogen dewars and technical problems experienced when vitrified embryos are transferred using non-surgical procedures have led to introduction alternative methods, such as preserving in state. This study evaluated whether a pH-stable medium containing high concentrations either foetal calf serum (FCS; 50%) or BSA (4%) combined with at temperatures 17 °C...
Abstract This study evaluated the effects of different concentrations (1, 10, 25, 50, and 100 µM) antioxidant N -(2-mercaptopropionyl)-glycine (NMPG), during culture in vitro-fertilized porcine oocytes. While highest NMPG (50 were toxic to developing embryos first two days culture, 25 µM achieved cleavage rates that similar those by control but did not sustain blastocyst production Day 7 culture. Compared medium, medium supplemented with 10 increased ( P < 0.05) formation, decreased...
In the context of porcine embryo transfer (ET) technology, understanding tightly regulated local uterine immune environment is crucial to achieve an adequate interaction between transferred embryos and receiving endometrium. However, information limited on status cyclic-recipient sows when during ET. The present study postulated that anti- proinflammatory cytokine profile 6 days after onset estrus differs endometria from uninseminated cyclic blastocyst-bearing sows. On Day cycle, endometrial...
Despite the reported promising farrowing rates after non-surgical and surgical transfers of vitrified porcine morulae blastocysts produced in vivo (range: 70–75%), pregnancy loss is 5–15 fold higher with than fresh embryos. The present study aimed to investigate whether vitrification affects transcriptome morulae, using microarrays RT-qPCR validation. Morulae were obtained surgically from weaned sows ( n = 13) on day 6 (day 0 estrus onset). A total 60 (treatment group). After 1 week storage,...
Seminal plasma (SP) in the female genital tract induces changes that affect multiple reproductive processes. One of active components SP is transforming growth factor β1 (TGF-β1), which has major roles embryo development and pregnancy. Embryo transfer (ET) technology welcomed by pig industry provided quality at collection as well fertility prolificacy recipients after ET increased. This study evaluated different intrauterine infusion treatments estrus (40 mL SP, TGF-β1 cytokine extender, or...