A5090819878- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Reproductive Physiology in Livestock
- Reproductive System and Pregnancy
- Animal Genetics and Reproduction
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Reproductive Health and Technologies
- CRISPR and Genetic Engineering
- MicroRNA in disease regulation
- Animal Behavior and Welfare Studies
- Neuroendocrine regulation and behavior
- Ovarian function and disorders
- Maternal Mental Health During Pregnancy and Postpartum
- Renal and related cancers
- Selenium in Biological Systems
- Genetic and phenotypic traits in livestock
- Effects and risks of endocrine disrupting chemicals
- Circular RNAs in diseases
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Virus-based gene therapy research
- Estrogen and related hormone effects
- Extracellular vesicles in disease
- Bioactive Compounds in Plants
- Stress Responses and Cortisol
Institute of Agrifood Research and Technology
2022-2025
Linköping University
2019-2024
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2024
Okayama University of Science
2023
Hospital de Galdakao
2023
Universidad de Murcia
2014-2022
Instituto Murciano de Investigación Biosanitaria
2017-2021
Abstract Surgical procedures are prevalent in porcine embryo transfer (ET) programs, where the use of vitrified embryos is quasi non-existent. This study compared effectiveness surgical vs nonsurgical deep uterine (NsDU) ET using vitrified, vivo -derived (morulae and blastocysts) on reproductive performance welfare recipients. The recipient sows (n = 122) were randomly assigned to one following groups: with 30 vitrified-warmed (S-30 group, control); NsDU-ET (NsDU-30 group) 40 (NsDU-40...
In the study presented here we identified transcriptomic markers for fertility in cargo of pig ejaculated spermatozoa using porcine-specific micro-arrays (GeneChip® miRNA 4.0 and GeneChip® Porcine Gene 1.0 ST). We report (i) relative abundance ssc-miR-1285, miR-16, miR-4332, miR-92a, miR-671-5p, miR-4334-5p, miR-425-5p, miR-191, miR-92b-5p miR-15b miRNAs, (ii) presence 347 up-regulated 174 down-regulated RNA transcripts high-fertility breeding boars, based on differences farrowing rate (FS)...
Abstract Semen modifies the expression of genes related to immune function along porcine female internal genital tract. Whether other pathways are induced by deposition spermatozoa and/or seminal plasma (SP), is yet undocumented. Here, determine their relative impact on uterine and tubal transcriptomes, microarray analyses were performed endocervix, endometrium endosalpinx collected from pre-ovulatory sows 24 h after either mating or artificial insemination (AI) with specific ejaculate...
In vitro embryo production (IVP) is of great importance to the porcine industry, as well for basic research and biomedical applications. Despite large efforts made in laboratories worldwide address suboptimal culture conditions, IVP remains inefficient. Nobiletin (Nob, 5,6,7,8,3´,4´ hexamethoxyflavone) supplementation (IVC) medium, enhances development various species. However, its impact on quality developmental capacity vitro-produced pig embryos yet be established. This study evaluated...
Background: Seminal plasma (SP) promotes sperm survival and fertilizing capacity, potentially affects embryo development, presumably via specific signaling pathways to the internal female genital tract. Objectives: This study evaluated how heterologous SP, infused immediately before postcervical artificial insemination (AI) affected development transcriptional pattern of pig endometria containing embryos. Materials Methods: Postweaning estrus sows (n = 34) received 40-mL intrauterine...
Pig embryo transfer (ET) is burdened by high mortality, with cytokines playing a significant role in recruitment of immune cells during attachment and placentation. We hereby tested if their levels endometrium placenta from sows carrying hemi-allogeneic (artificially inseminated sows; C+ positive control) or allogeneic embryos (sows subjected to ET; ET) peri-implantation (D18) post-implantation (D24) are suitable mirrors rejection tolerance after ET. Non-pregnant (C−) were used as negative...
The infusion of boar seminal plasma (SP) before artificial insemination (AI) positively alters the expression endometrial genes and pathways involved in embryo development. This study aimed to determine which transcriptome changes occur preimplantation embryos response SP infusions during estrus. Postweaning estrus sows received 40-mL intrauterine either (N = 6) or BTS extender (control group; N 30 min each two post-cervical AIs. On Day 6, were surgically collected analyzed for differential...
The oil overlay micro-drop system is widely used for cultures of mammalian gametes and embryos. We evaluated hereby the effects two unaltered commercial oils- Sigma mineral (S-MO) Nidoil paraffin (N-PO)-on in vitro embryo production (IVP) outcomes using a pig model. results showed that while either apparently did not affect oocyte maturation fertilization rates, S-MO negatively affected cleavage blastocyst formation and, consequently, total efficiency system. No differences oxidation state...
Abstract The use of pH-stable media would simplify embryo vitrification and the warming porcine embryos might facilitate application transfer in practice. In this work, we investigated whether a basal medium constituted Tyrode’s lactate medium, polyvinyl alcohol, HEPES for buffering was suitable place conventional gas-equilibrated media. A high percentage (>90%) survived achieving vitro survival rates similar to vitrified-warmed using protocol their fresh counterparts. did not affect vivo...
Abstract The coenzyme Q10 (CoQ10) is a potent antioxidant with critical protection role against cell oxidative stress, caused by the mitochondrial dysfunction. This study evaluated effects of CoQ10 supplementation to in vitro maturation (IVM) or embryo culture media on maturation, fertilization and subsequent embryonic development pig oocytes embryos. Maturation (Experiment 1) 2) were supplemented 0 (control), 10, 25, 50 100 μM CoQ10. addition 10–50 IVM medium did not affect percentage MII...
This study was designed to investigate the impact of vitrification on transcriptome profile blastocysts using a porcine (Sus scrofa) model and microarray approach. Blastocysts were collected from weaned sows (n = 13). A total 60 vitrified (treatment group). After warming, embryos cultured in vitro for 24 h. Non-vitrified 40) used as controls. culture period, embryo viability morphologically assessed. 30 viable per group (three pools 10 4 different donors each) subjected gene expression...
The epididymis is responsible for peripheral immune tolerance of maturing spermatozoa even though these have xeno-antigens foreign to the male and female system. also produces factors required fertilization serves as a sperm repository until time ejaculation. These reproduction-relevant epididymal functions occur in mesonephros-derived duct-system that composed absorptive secretory epithelial cells with capacity merocrine apocrine secretion proteins, antioxidative- electrolyte/pH-regulating...
Abstract During pregnancy, the immune system is modified to allow developmental tolerance of semi-allogeneic fetus and placenta term. Pregnant women suffering from stress, anxiety, depression show dysfunctions their that may be responsible for fetal and/or newborn disorders, provided placental gene regulation compromised. The present study explored effects maternal chronic self-perceived during pregnancy on expression immune-related genes pathways in term placenta. Pregnancies were...