A5045128271- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Reproductive Physiology in Livestock
- Reproductive biology and impacts on aquatic species
- Animal Genetics and Reproduction
- Reproductive System and Pregnancy
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genetic and phenotypic traits in livestock
- Ovarian function and disorders
- Seed Germination and Physiology
- Bee Products Chemical Analysis
- Cancer-related molecular mechanisms research
- Estrogen and related hormone effects
- Ion Transport and Channel Regulation
- Rabbits: Nutrition, Reproduction, Health
- Proteoglycans and glycosaminoglycans research
- Animal Nutrition and Physiology
- MicroRNA in disease regulation
- Urologic and reproductive health conditions
- Livestock and Poultry Management
- Extracellular vesicles in disease
- Epigenetics and DNA Methylation
- Nerve Injury and Rehabilitation
- Silkworms and Sericulture Research
- Peripheral Nerve Disorders
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2022-2025
Institute of Agrifood Research and Technology
2023-2025
Universitat Autònoma de Barcelona
2018-2025
Linköping University
2015-2024
Instituto de Investigación en Ciencias de la Alimentación
2024
Consejo Superior de Investigaciones Científicas
2022-2023
Universidad de León
2010-2023
Chattogram Veterinary and Animal Sciences University
2020
Hospital de León
2019
Mating or cervical deposition of spermatozoa seminal plasma (SP) modifies the expression genes affecting local immune defense processes at oviductal sperm reservoir in animals with internal fertilization, frequently by down-regulation. Such responses may occur alongside transport to even beyond reservoir. Here, immune-related gene was explored cDNA microarrays on porcine cervix-to-infundibulum tissues, pre-/peri-ovulation. Samples were collected 24 h post-mating sperm-peak SP (from fraction...
This study aimed to examine whether the addition of glutathione ethyl ester (GSH-OEt) in vitro maturation (IVM) medium would improve resilience bovine oocytes withstand vitrification. The effects GSH-OEt on spindle morphology, levels reactive oxygen species (ROS), mitochondrial activity and distribution, embryo developmental potential were assessed together with expression genes a role apoptosis (BAX, BCL2), oxidative-stress pathways (GPX1, SOD1), water channels (AQP3), implantation (IFN-τ)...
The aim of our study was to examine the effects crocin (0.5 (C0.5), 1 (C1) and 1.5 (C1.5) mM) naringenin (50 (N50), 100 (N100) 150 (N150) μM) in cryopreservation extender for freezing rooster semen. Sperm motility, viability, abnormalities, membrane functionality, active mitochondria, apoptosis status, lipid peroxidation (LP), GPX, SOD, TAC, mRNA expression pro-apoptotic (CASPASE 3) anti-apoptotic (Bcl-2) genes, fertile eggs, hatched eggs hatching rate were investigated following...
In the study presented here we identified transcriptomic markers for fertility in cargo of pig ejaculated spermatozoa using porcine-specific micro-arrays (GeneChip® miRNA 4.0 and GeneChip® Porcine Gene 1.0 ST). We report (i) relative abundance ssc-miR-1285, miR-16, miR-4332, miR-92a, miR-671-5p, miR-4334-5p, miR-425-5p, miR-191, miR-92b-5p miR-15b miRNAs, (ii) presence 347 up-regulated 174 down-regulated RNA transcripts high-fertility breeding boars, based on differences farrowing rate (FS)...
The protective role of astaxanthin nanoparticles (Ast NPs, 25 mg/kg p.o) against cadmium (Cd, 1 mg/100 g b.w. SC), a known inductor lipid peroxidation and changes in the antioxidant defense system Ross 308 breeder roosters sperm, was examined. Sperm motility (computer-assisted sperm analysis), membrane integrity (hypoosmotic swelling test), viability, total abnormality, enzymatic parameters were assessed after thawing. testis/body weight (mg/kg) ratio HE staining results testis also...
Abstract Semen modifies the expression of genes related to immune function along porcine female internal genital tract. Whether other pathways are induced by deposition spermatozoa and/or seminal plasma (SP), is yet undocumented. Here, determine their relative impact on uterine and tubal transcriptomes, microarray analyses were performed endocervix, endometrium endosalpinx collected from pre-ovulatory sows 24 h after either mating or artificial insemination (AI) with specific ejaculate...
Thawed ram spermatozoa were incubated at 37°C in the presence of dehydroascorbic acid (DHA), TEMPOL (TPL), N-acetyl-cysteine (NAC) and rutin (RUT), 0.1 1 mm, order to test their effects on sperm physiology. Cryopreserved from four rams thawed, pooled, washed TALP-Hepes with mm or each antioxidant, performing a replicate induced oxidative stress (Fe(2+) /ascorbate). Motility (CASA), viability mitochondrial membrane potential (flow cytometry) analysed 2 4 h. Lipoperoxidation (MDA production),...
Spermatozoa are stored in the oviductal functional sperm reservoir animals with internal fertilization, including zoologically distant classes such as pigs or poultry. They held fertile for times ranging from a couple of days (in pigs), to several weeks chickens), before they gradually released fertilize newly ovulated eggs. It is currently unknown whether females these species share conserved mechanisms tolerate lengthy presence immunologically-foreign spermatozoa. Therefore, global gene...
Background: Seminal plasma (SP) promotes sperm survival and fertilizing capacity, potentially affects embryo development, presumably via specific signaling pathways to the internal female genital tract. Objectives: This study evaluated how heterologous SP, infused immediately before postcervical artificial insemination (AI) affected development transcriptional pattern of pig endometria containing embryos. Materials Methods: Postweaning estrus sows (n = 34) received 40-mL intrauterine...
Pig embryo transfer (ET) is burdened by high mortality, with cytokines playing a significant role in recruitment of immune cells during attachment and placentation. We hereby tested if their levels endometrium placenta from sows carrying hemi-allogeneic (artificially inseminated sows; C+ positive control) or allogeneic embryos (sows subjected to ET; ET) peri-implantation (D18) post-implantation (D24) are suitable mirrors rejection tolerance after ET. Non-pregnant (C−) were used as negative...
The infusion of boar seminal plasma (SP) before artificial insemination (AI) positively alters the expression endometrial genes and pathways involved in embryo development. This study aimed to determine which transcriptome changes occur preimplantation embryos response SP infusions during estrus. Postweaning estrus sows received 40-mL intrauterine either (N = 6) or BTS extender (control group; N 30 min each two post-cervical AIs. On Day 6, were surgically collected analyzed for differential...
A combined Genotyping By Sequencing (GBS) and methylated DNA immunoprecipitation (MeDIP) protocol was used to identify—in parallel—genetic variation (Genomic-Wide Association Studies (GWAS) epigenetic differences of Differentially Methylated Regions (DMR) in the genome spermatozoa from porcine animal model. Breeding boars with good semen quality (n = 11) specific well-documented fertility (farrowing rate, FR) prolificacy (litter size, LS) 7) artificial insemination programs, using FR LS,...
Cattle populations are highly amenable to the genetic mapping of male reproductive traits because longitudinal data on ejaculate quality and dense microarray-derived genotypes available for thousands artificial insemination bulls. Two young Nordic Red bulls delivered sperm with low progressive motility (i.e., asthenospermia) during a semen collection period more than four months. The were related through common ancestor both their paternal maternal ancestry. Thus, recessive mode inheritance...