A5031275732- Genetic and phenotypic traits in livestock
- Genetic Mapping and Diversity in Plants and Animals
- Animal Genetics and Reproduction
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- Plant Reproductive Biology
- Skin and Cellular Biology Research
- Reproductive Biology and Fertility
- T-cell and B-cell Immunology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant and fungal interactions
- Genomics and Phylogenetic Studies
- Liver Disease Diagnosis and Treatment
- Sperm and Testicular Function
- Hepatitis B Virus Studies
- Molecular Biology Techniques and Applications
- Milk Quality and Mastitis in Dairy Cows
- Hepatitis C virus research
- Renal and related cancers
- CAR-T cell therapy research
- Vector-Borne Animal Diseases
- Genetic Syndromes and Imprinting
- RNA Interference and Gene Delivery
- Genetics and Plant Breeding
- Mycotoxins in Agriculture and Food
Technical University of Munich
2015-2025
Institute of Ethnology
2024
Institute of Animal Physiology of the Slovak Academy of Sciences
2024
Universität Hamburg
2024
University Medical Center Hamburg-Eppendorf
2024
Weihenstephan-Triesdorf University of Applied Sciences
2019
ABSTRACT Extracellular vesicles (EVs) are intercellular communicators with key functions in physiological and pathological processes have recently garnered interest because of their diagnostic therapeutic potential. The past decade has brought about the development commercialization a wide array methods to isolate EVs from serum. Which subpopulations captured strongly depends on isolation method, which turn determines how suitable resulting samples for various downstream applications. To...
We carried out whole genome resequencing of 127 chicken including red jungle fowl and multiple populations commercial broilers layers to perform a systematic screening adaptive changes in modern (Gallus gallus domesticus). uncovered >21 million high quality SNPs which 34% are newly detected variants. This panel comprises >115,000 predicted amino-acid altering substitutions as well 1,100 be stop-gain or -loss, several reach frequencies. Signatures selection were investigated both through...
Genetic variants underlying reduced male reproductive performance have been identified in humans and model organisms, most of them compromising semen quality. Occasionally, fertility is severely compromised although analysis remains without any apparent pathological findings (i.e., idiopathic subfertility). Artificial insemination (AI) cattle populations requires close examination all ejaculates before insemination. Although anomalous are rejected, success varies considerably among AI bulls....
Milk composition traits exhibit a complex genetic architecture with small number of major quantitative trait loci (QTL) explaining large fraction the variation and numerous QTL minor effects. In order to identify for milk fat percentage (FP) in German Holstein-Friesian (HF) population, genome-wide association study (GWAS) was performed. The population consisted 2327 progeny-tested bulls. Genotypes were available 44,280 SNPs. Phenotypes form estimated breeding values (EBVs) FP used as highly...
Cattle breeding populations are susceptible to the propagation of recessive diseases. Individual sires generate tens thousands progeny via artificial insemination. The frequency deleterious alleles carried by such may increase considerably within few generations. Deleterious manifest themselves often missing homozygosity resulting from embryonic/fetal, perinatal or juvenile lethality homozygotes. A scan for homozygous haplotype deficiency in 25,544 Fleckvieh cattle uncovered four haplotypes...
In humans, the clinical and molecular characterization of sporadic syndromes is often hindered by small number patients difficulty in developing animal models for severe dominant conditions. Here we show that availability large data sets whole-genome sequences, high-density SNP chip genotypes extensive recording phenotype offers an unprecedented opportunity to quickly dissect genetic architecture conditions livestock. We report on identification seven de novo mutations CHD7, COL1A1, COL2A1,...
Resident T lymphocytes (T RM ) protect tissues during pathogen reexposure. Although phenotype and restricted migratory pattern are established, we have a limited understanding of their response kinetics, stability, turnover reinfections. Such characterizations been by the absence in vivo fate-mapping systems. We generated two mouse models, one to stably mark CD103 + cells (a marker cells) other specifically deplete − cells. Using these observed that intestinal became activated viral or...
Haplotypes with reduced or missing homozygosity may harbor deleterious alleles that compromise juvenile survival. A scan for homozygous haplotype deficiency revealed a short segment on bovine chromosome 19 (Braunvieh 2, BH2) was associated high mortality in Braunvieh cattle. However, the molecular genetic underpinnings and pathophysiology of BH2 remain to be elucidated.The frequency 6.5 % 8,446 animals from national genome databases. Both perinatal calves were higher than average cattle...
T cell maintenance in chronic infection and cancer follows a hierarchical order. Short-lived effector CD8 cells are constitutively replaced from proliferation-competent Tcf1-expressing progenitor population. This occurs spontaneously at low levels increases magnitude upon blocking PD-1 signaling. We explore how CD4 help controls transition survival of the progenitors their progeny by utilizing single-cell RNA sequencing. Unexpectedly, absence caused reductions numbers only among terminally...
Background: Artificial insemination is widely used in many cattle breeding programs.Semen samples of bulls are collected and closely examined immediately after collection at artificial centers.Only ejaculates without anomalous findings retained for insemination.Although morphological aberrations the spermatozoa a frequent reason discarding ejaculates, genetic determinants underlying poor semen quality scarcely understood.Results: A tail stump sperm defect was observed three Swedish Red...
Inherited developmental diseases can cause severe animal welfare and economic problems in dairy cattle. The use of a small number bulls for artificial insemination (AI) carries risk that recessive defects rapidly enrich the population. In recent years, an increasing Finnish Ayrshire calves have been identified with signs ptosis, intellectual disability, retarded growth mortality, which constitute inherited disorder classified as PIRM syndrome. We established cohort nine PIRM-affected 38...
Genotyping of sequence variants typically involves, as a first step, the alignment sequencing reads to linear reference genome. Because genome represents only small fraction all DNA variation within species, allele bias may occur at highly polymorphic or divergent regions Graph-based methods facilitate comparison variation-aware graph, which incorporates collection non-redundant sequences that segregate species. We compared accuracy and sensitivity graph-based variant genotyping using...
The characterization of T cells in chickens has proven to be challenging, primarily due the lack specific markers differentiate classical Th1, Th2, and Th17 subsets. Furthermore, possess a notably high proportion γδ cells, making them unique model for investigating poorly understood role these not only but also mammals. To gain deeper insights into functions characteristics αβ cell subsets chickens, whole transcriptome analysis (WTA) on CD3+ single isolated from TCR Cβ knockout (KO), Cγ KO,...
Abstract Anti-viral immunity can vary tremendously from individual to but mechanistic understanding is still scarce. Here, we show that a defined, low complex bacterial community (OMM 12 ) not the general absence of microbes in germ-free mice leads more potent immune response compared microbiome specific-pathogen-free (SPF) after systemic viral infection with LCMV Clone-13. Consequently, gnotobiotic colonized OMM have severe LCMV-induced disease pathology also enhance clearance intestinal...
Abstract Background Hitchhiking mapping and association studies are two popular approaches to map genotypes phenotypes. In this study we combine both complement their specific strengths weaknesses, resulting in a method with higher statistical power fewer false positive signals. We applied our approach dairy cattle as they underwent extremely successful selection for milk production traits since an excellent phenotypic record is available. performed whole genome tests new mixed model account...
Abstract The ongoing progress in primordial germ cell derivation and cultivation is opening new ways reproductive biotechnology. This study tested whether functional sperm cells can be matured from genetically manipulated after transplantation adult testes used to restore fertility. We show that spermatogenesis restored mCherry-expressing or GFP-expressing are transplantated into the of sterilized G 0 roosters mCherry-positive GFP-positive non-chimeric transgenic 1 offspring efficiently...
The widespread use of individual sires for artificial insemination promotes the propagation recessive conditions. Inadvertent matings between unnoticed carriers deleterious alleles may result in manifestation fatal phenotypes their progeny. Breeding consultants and farmers reported on Vorderwald calves with a congenital skin disease. clinical findings affected were compatible epidermolysis bullosa. Pedigree analysis indicated autosomal inheritance bullosa cattle. We genotyped two diseased 41...
Cattle populations are highly amenable to the genetic mapping of male reproductive traits because longitudinal data on ejaculate quality and dense microarray-derived genotypes available for thousands artificial insemination bulls. Two young Nordic Red bulls delivered sperm with low progressive motility (i.e., asthenospermia) during a semen collection period more than four months. The were related through common ancestor both their paternal maternal ancestry. Thus, recessive mode inheritance...
Abstract Plants exhibit impressive genetic and chemical diversity, not just between species but also within species, the importance of plant intraspecific variation for structuring ecological communities is well known. When there at local population level, this can create a spatially heterogeneous habitat specialised herbivores potentially leading to non‐random distribution individuals across host plants. Plant affect directly indirectly via third resulting in variable herbivore growth rates...