A5066760714- melanin and skin pigmentation
- Cutaneous Melanoma Detection and Management
- Viral Infectious Diseases and Gene Expression in Insects
- Animal Genetics and Reproduction
- Melanoma and MAPK Pathways
- Animal Nutrition and Physiology
- Genetic and phenotypic traits in livestock
- Immunotherapy and Immune Responses
- Molecular Biology Techniques and Applications
- Immune Cell Function and Interaction
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- Microbial infections and disease research
- Animal Disease Management and Epidemiology
- CAR-T cell therapy research
- Advanced Biosensing Techniques and Applications
- Inflammatory Myopathies and Dermatomyositis
- Genetic Mapping and Diversity in Plants and Animals
- Mast cells and histamine
- Mycobacterium research and diagnosis
- Livestock and Poultry Management
- Cell Image Analysis Techniques
- Cancer Cells and Metastasis
- RNA Interference and Gene Delivery
- Peroxisome Proliferator-Activated Receptors
Génétique Animale et Biologie Intégrative
2012-2024
Département Génétique Animale
2003-2024
Institut de Radiobiologie Cellulaire et Moléculaire
2011-2024
Université Paris-Saclay
2015-2023
AgroParisTech
2011-2023
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2002-2023
CEA Paris-Saclay
2017-2022
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2011-2022
Université Paris Cité
2022
Yangzhou University
2020
In humans, the clinical and molecular characterization of sporadic syndromes is often hindered by small number patients difficulty in developing animal models for severe dominant conditions. Here we show that availability large data sets whole-genome sequences, high-density SNP chip genotypes extensive recording phenotype offers an unprecedented opportunity to quickly dissect genetic architecture conditions livestock. We report on identification seven de novo mutations CHD7, COL1A1, COL2A1,...
High-Z metallic nanoparticles (NPs) are new players in the therapeutic arsenal against cancer, especially radioresistant cells. Indeed, presence of these NPs inside malignant cells is believed to enhance effect ionizing radiation by locally increasing dose deposition. In this context, potential platinum (PtNPs) as radiosensitizers was investigated two breast cancer cell lines, T47D and MDA-MB-231, showing a different sensitivity. PtNPs were internalized lines localized lysosomes...
Radio-enhancement is a promising anti-cancer approach based on local radiation dose increase due to the presence of metallic nanoparticles (NPs) within cancer cells. Depending their composition, size and cellular properties, NPs can follow multiple pathways entry routes. We observed that gold, platinum TiO2 are internalized at higher levels in mesenchymal cells compared epithelial breast models. A global survey gene expression between exposed 4 different NP types revealed an involvement...
Androstenone is one of the main compounds responsible for boar taint, and 3beta-hydroxysteroid dehydrogenase (3betaHSD) might be involved in its metabolism. In this study, gene expression 3betaHSD 17beta-hydroxysteroid (17betaHSD) were determined by real-time PCR analysis related to concentrations androstenone, testosterone, estrone sulphate (E1S). The experiments performed on gonadally intact male pigs classified based high or low fat androstenone concentrations, as predetermined HPLC, well...
Abstract Allelic imbalance is a common phenomenon in mammals that plays an important role gene regulation. An Allele Specific Expression (ASE) approach can be used to detect variants with cis -regulatory effect on expression. In cattle, this type of study has only been done once Holstein. our we performed genome-wide analysis ASE 19 Limousine muscle samples. We identified 5,658 SNPs (Single Nucleotide Polymorphisms showing allele specific expression) 13% genes detectable expression the...
Cancer stem cells (CSCs) serve an essential role in failure of conventional antitumor therapy. In breast cancer, CD24‑/low/CD44+ phenotype and high aldehyde dehydrogenase activity are associated with CSC subtypes. Furthermore, pattern is also characteristic mesenchymal generated by epithelial‑mesenchymal transition (EMT). CD24 a surface marker expressed numerous types tumor, however, its biological functions cancer progression treatment resistance remain poorly documented. Loss expression...
Immunological research in pigs benefits from many improvements with a direct impact on the veterinary control of pig husbandry and biomedical models. We compiled available knowledge to develop gating strategies monitor simultaneously all blood immune cell types by multicolor flow cytometry Melanoblastoma-bearing Libechov Minipigs (MeLiM). The MeLiM spontaneously develops cutaneous melanomas that regress few months later. monitored lymphoid myeloid subsets 3 21 week-old pigs. Interestingly,...
Despite major advances, it is estimated that a large part of melanoma predisposing genes remains to be discovered. Animal models spontaneous diseases are valuable tools and experimental crosses can used identify fine-map new susceptibility loci associated with melanoma. We performed Genome-Wide Association Study (GWAS) occurrence progression (clinical ulceration presence metastasis) in porcine model melanoma, the MeLiM pig. Five on chromosomes 2, 5, 7, 8 16 showed genome-wide significant...
Abstract The human cutaneous metastatic melanoma is the deadliest skin cancer. Partial, or less frequently complete spontaneous regressions could be observed, mainly mediated by T cells. Nevertheless, underlying mechanisms are not fully unraveled. We investigated first events of immune response related to cancer regression in Melanoma-bearing Libechov Minipigs (MeLiM), a unique swine model that regresses spontaneously. Using multiparameter flow cytometry strategy and integrating new clinical...
Summary Our aim was to analyse the transcription levels of three non‐classical class Ib genes SLA‐6 , SLA‐7 and SLA‐8 swine major histocompatibility complex in various tissues conditions compare them classical Ia genes. Twenty‐five adult from two pig breeds, renal PK15 cells infected with Pseudorabies virus, peripheral blood mononuclear (PBMCs) stimulated by lipopolysaccharide or a mixture phorbol myristate acetate ionomycin were included our study. Relative quantified quantitative real‐time...
Summary KIT mutations have been detected in different cancer subtypes, including melanoma. The gene also has extensively studied farm animals for its prominent role coat color. present work aimed at detecting variants a porcine model of cutaneous melanoma, the melanoblastoma‐bearing L ibechov M inipig ( e i ). By sequencing exons and intron borders, 36 SNP s one indel were identified. Of 10 coding s, three non‐synonymous mutations, likely to affect protein conformation. A promising variant,...
In pig, very little information is available on the non classical class I (Ib) genes of Major Histocompatibility Complex (MHC) i.e. SLA-6, -7 and -8. Our aim was to focus transcription pattern SLA-7 gene. RT-PCR experiments were carried out with specific primers targeting either full coding sequence (CDS) from exon 1 3 prime untranslated region (3UTR) or a partial CDS 4 3UTR. We show that gene expresses length transcript not yet identified refines annotation eight exons instead seven as...
Abstract In MeLiM minipigs, melanomas develop around birth, can metastasize, and have histopathologic characteristics similar to humans. Interestingly, eventually regress. This favorable outcome raises the question of their malignancy, which we investigated. We clinically followed tens tumors from onset first signs regression. Transcriptome analysis revealed an enrichment all cancer hallmarks in melanomas, although no activating or suppressing somatic mutation were found common driver genes....