A5057915994- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Animal Disease Management and Epidemiology
- Herpesvirus Infections and Treatments
- Immune Response and Inflammation
- interferon and immune responses
- Cytomegalovirus and herpesvirus research
- Aquaculture disease management and microbiota
- Cell Adhesion Molecules Research
- Peptidase Inhibition and Analysis
- RNA Interference and Gene Delivery
- Galectins and Cancer Biology
- Influenza Virus Research Studies
Technical University of Munich
2023-2025
Avian γδ T lymphocytes are highly abundant in the intestinal mucosa and play a critical role immune defense against infectious diseases chickens. However, their specific contributions to infection control remain poorly understood. To investigate of cells possible compensation, we studied wild-type cell knockout chickens following with Salmonella Enteritidis. Bacterial loads liver, cecal content, wall were quantified. Immune populations blood, spleen, cecum analyzed using flow cytometry. gene...
The characterization of T cells in chickens has proven to be challenging, primarily due the lack specific markers differentiate classical Th1, Th2, and Th17 subsets. Furthermore, possess a notably high proportion γδ cells, making them unique model for investigating poorly understood role these not only but also mammals. To gain deeper insights into functions characteristics αβ cell subsets chickens, whole transcriptome analysis (WTA) on CD3+ single isolated from TCR Cβ knockout (KO), Cγ KO,...
Abstract The rapid cross-species transmission of highly pathogenic avian influenza presents a significant zoonotic threat. Elucidating the interferon (IFN) system, primary antiviral defense in chickens, is critical for controlling virus at its source and preventing spillover into humans other species. We engineered type I (IFN-α/β) III (IFN-λ) IFN receptor knockout chickens to dissect role IFNs viral infections. Results revealed that predominantly modulates innate immune cell populations, T...
Avian γδ T lymphocytes are highly abundant in the intestinal mucosa and play a critical role immune defense against infectious diseases chickens. However, their specific contributions to infection control remain poorly understood. To investigate of cells possible compensation, we studied wild-type cell knockout chickens following with Salmonella Enteritidis. Bacterial loads liver, cecal content, wall were quantified. Immune populations blood, spleen, cecum analyzed using flow cytometry. gene...
ABSTRACT Marek’s disease virus (MDV) is an oncogenic alphaherpesvirus that causes deadly lymphomas in chickens. In chickens, up to 50% of all peripheral T cells are gamma delta (γδ) cells. Until now, their role MDV pathogenesis and tumor formation remains poorly understood. To investigate the γδ pathogenesis, we infected recently generated cell knockout chickens with very virulent MDV. Strikingly, incidence were highly increased absence cells, indicating play important immune response...
The availability of genetically modified mice has facilitated the study mammalian T cells. No model yet been developed to these cells in chickens, an important livestock species with a high γδ To investigate role and αβ cell populations birds, we generated chickens lacking populations. This was achieved by genomic deletion constant region receptor γ or β chain, leading complete loss either Our results show that but not resulted severe phenotype KO chickens. exhibited granulomas associated...
Abstract Retinoic acid-inducible gene I ( RIG-I ) activates mitochondrial antiviral signaling proteins, initiating the response. and RNF135 , a ubiquitin ligase regulator, are missing in domestic chickens but conserved mallard ducks. The chickens’ loss was long believed to be linked increased avian influenza susceptibility. We reinstated both genes examined their susceptibility infection with an H7N1 virus. Uninfected -expressing exhibited shifts T B cells. At same time, led severe disease,...
The development of functional B lymphocytes during chicken embryogenesis relies on a series tightly regulated processes. Precursor cells migrate from the spleen via blood to bursa Fabricius, where they colonize bursal follicles undergo further maturation and differentiation. To better understand molecular mechanisms underlying early cell migration in embryo, transcriptome analysis isolated spleen, blood, at embryonic days (ED) 12, ED14, ED16 was performed. These findings suggest that...
Abstract The availability of genetically modified mice has facilitated the study mammalian T cells. No model yet been developed to these cells in chicken, an important livestock species with a high γδ To investigate role and αβ cell populations birds, we generated chickens lacking populations. This was achieved by genomic deletion constant region receptor γ or chain, leading complete loss either Our results show that but not resulted severe phenotype knockout chickens. exhibited granulomas...
Abstract Marek’s disease virus (MDV) is an oncogenic alphaherpesvirus that causes deadly T cell lymphomas in chickens. MDV highly associated which allows the to evade antibody-mediated neutralization. Therefore, cell-mediated immune responses are thought be crucial for combating this pathogen. In chickens, gamma delta (γδ) cells represent a major population with up 50% of all peripheral cells. However, their role pathogenesis and tumor formation remains poorly understood. To investigate γδ...