A5063560779- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Microbial infections and disease research
- Mycobacterium research and diagnosis
- Animal Disease Management and Epidemiology
- Clostridium difficile and Clostridium perfringens research
- Tuberculosis Research and Epidemiology
- Medical Device Sterilization and Disinfection
- Salmonella and Campylobacter epidemiology
- Yersinia bacterium, plague, ectoparasites research
- Vector-borne infectious diseases
- Immune Cell Function and Interaction
- Antimicrobial agents and applications
- Zoonotic diseases and public health
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Animal health and immunology
- Bacillus and Francisella bacterial research
- Veterinary medicine and infectious diseases
- Reproductive tract infections research
- Antibiotic Resistance in Bacteria
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Occupational exposure and asthma
- Rabies epidemiology and control
Friedrich-Loeffler-Institut
2016-2025
Département Santé Animale
2021
Genomics (United Kingdom)
2021
Animal Health Research Institute
2021
Freie Universität Berlin
2017
Robert Koch Institute
2017
Wellcome Sanger Institute
2017
Justus-Liebig-Universität Gießen
2001-2015
Institut für Hygiene und Umwelt
2006-2008
This research was funded by EU FP7 grant ANTIGONE (#278976). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Abstract Background The epidemiological situation of ovine chlamydial infections in continental Europe, especially Germany is poorly characterised. Using the German state Thuringia as a model example, sero- and antigen prevalence was estimated thirty-two randomly selected sheep flocks with an average abortion rate lower than 1%. Seven vaccinated were reviewed separately. Results A wide range samples from 32 examined. Assumption seroprevalence 10% (CI 95%) at flock level, revealed that 94%...
Abstract Background Escherichia coli is an opportunistic pathogen which colonizes various host species. However, to what extent genetic lineages of E. are adapted or restricted specific hosts and the genomic determinants such adaptation restriction poorly understood. Results We randomly sampled isolates from four countries (Germany, UK, Spain, Vietnam), obtained five species (human, pig, cattle, chicken, wild boar) over 16 years, both healthy diseased hosts, construct a collection 1198...
Although cattle develop humoral immune responses to Shiga-toxigenic (Stx+) Escherichia coli O157:H7, infections often result in long-term shedding of these human pathogenic bacteria. The objective this study was compare and cellular Stx+ Stx- E. O157:H7. Three groups calves were inoculated intrarumenally, twice a 3-week interval, with different strains coli: Stx2-producing O157:H7 strain (Stx2+ O157), Shiga toxin-negative (Stx- or nonpathogenic (control). Fecal Stx2+ O157 significantly...
The pestivirus bovine viral diarrhea virus (BVDV) was shown to bind the CD46 molecule, which subsequently promotes entry of virus. To assess receptor usage BVDV type 1 (BVDV-1) and BVDV-2, 30 isolates including clinical samples were assayed for their sensitivity anti-CD46 antibodies. With a single exception infectivity all tested strains BVDV-1 BVDV-2 inhibited by antibodies, indicates general as receptor. Molecular analysis interaction between BVD virion performed mapping binding site on...
ABSRTACT Coxiella burnetii is an obligate intracellular bacterium that causes Query (Q) fever, a zoonotic disease. It requires functional type IV secretion system (T4SS) which translocate bacterial effector proteins into the host cell cytoplasm and thereby facilitates replication. To date, more than 130 have been identified, but their functions remain largely unknown. Recently, we demonstrated one of these proteins, CaeA (CBU1524) localized to nucleus inhibited intrinsic apoptosis HEK293 or...
Antimicrobial resistance (AMR) is considered one of the greatest threats to both human and animal health. Efforts address AMR include implementing antimicrobial stewardship programs introducing alternative treatment options. Nevertheless, effective infectious diseases caused by bacteria will still require identification development new agents. Eight different natural products were tested for activity against seven pathogenic bacterial species (Brachyspira sp., Chlamydia Clostridioides...
ABSTRACT Shiga toxin-producing Escherichia coli (STEC) is widespread in the cattle population, but clinical significance of toxins (Stx’s) for bovine species remains obscure. Since Stx’s exert immunomodulating effects other species, we examined effect purified Stx1 on a B lymphoma cell line (BL-3) and peripheral blood mononuclear cells (PBMC) isolated from adult by viability assays flow cytometry analysis. markedly induced apoptosis stimulated BL-3 cells. The susceptibility this...
Shiga toxin-producing Escherichia coli (STEC) strains can colonize cattle for several months and may, thus, serve as gene reservoirs the genesis of highly virulent zoonotic enterohemorrhagic E. (EHEC). Attempts to reduce human risk acquiring EHEC infections should include strategies control such STEC persisting in cattle. We therefore aimed identify genetic patterns associated with colonization type bovine host. included 88 persistent colonizing (STEC(per)) (shedding ≥4 months) 74...
Avian γδ T lymphocytes are highly abundant in the intestinal mucosa and play a critical role immune defense against infectious diseases chickens. However, their specific contributions to infection control remain poorly understood. To investigate of cells possible compensation, we studied wild-type cell knockout chickens following with Salmonella Enteritidis. Bacterial loads liver, cecal content, wall were quantified. Immune populations blood, spleen, cecum analyzed using flow cytometry. gene...
ABSTRACT Antimicrobial resistance threatens human and animal health, with antimicrobial usage being a key driver of selection, transmission, spread resistant bacteria. Livestock represents potential reservoir for leading authorities to restrict veterinary fluoroquinolones certain cephalosporins. However, growing evidence indicates that the corresponding determinants can be retained even in drugs’ absence. To obtain data on magnitude dynamics this phenomenon pig farming, we quantitatively...
Avian γδ T lymphocytes are highly abundant in the intestinal mucosa and play a critical role immune defense against infectious diseases chickens. However, their specific contributions to infection control remain poorly understood. To investigate of cells possible compensation, we studied wild-type cell knockout chickens following with Salmonella Enteritidis. Bacterial loads liver, cecal content, wall were quantified. Immune populations blood, spleen, cecum analyzed using flow cytometry. gene...
Tuberculosis (TB), caused by Mycobacterium tuberculosis, is the world's leading cause of mortality from a single bacterial pathogen. With increasing frequency, emergence drug-resistant mycobacteria leads to failures standard TB treatment regimens. Therefore, new anti-TB drugs are urgently required. BTZ-043 belongs novel class nitrobenzothiazinones, which inhibit mycobacterial cell wall formation covalent binding an essential cysteine in catalytic pocket decaprenylphosphoryl-β-d-ribose...
ABSTRACT Factors and mechanisms determining the differences in virulence host specificity between zoonotic agents Chlamydia psittaci abortus are still largely unknown. In present study, two strains were compared for their invasiveness, virulence, capability of eliciting an immune response chicken embryos. On breeding day 10, embryonated eggs inoculated with 5 × 10 4 inclusion-forming units. As shown by immunohistochemistry quantitative real-time PCR, C. displayed a significantly better...
ABSTRACT Bovine colonic crypt cells express CD77 molecules that potentially act as receptors for Shiga toxins (Stx). The implication of this finding the intestinal colonization cattle by human pathogenic Stx-producing Escherichia coli (STEC) remains undefined. We used flow cytometric and real-time PCR analyses primary cultures to evaluate cell viability, expression, gene transcription in presence absence purified Stx1. A subset cultured epithelial had Stx which were located mainly...
Ruminants are the main source of human infections with obligate intracellular bacterium Coxiella (C.) burnetii. Infected animals shed high numbers C. burnetii by milk, feces, and birth products. In goats, shedding latter route coincides replication in epithelial (trophoblast) cells placenta, which led us to hypothesize that generally implicated We therefore aimed at analyzing interactions bovine host (1) entry site (lung epithelium) govern immune responses (2) gut, udder placenta decisive...
ABSTRACT Although domestic ruminants have long been recognized as the main source of human Q fever, little is known about lifestyle that obligate intracellular Gram-negative bacterium Coxiella burnetii adopts in its animal host. Because macrophages are considered natural target cells pathogen, we established primary bovine monocyte-derived (MDM) an vitro infection model to study reservoir host-pathogen interactions at cellular level. In addition, alveolar were included take cell type...
Most human Q fever infections originate from small ruminants. By contrast, highly prevalent shedding of Coxiella (C.) burnetii by bovine milk rarely results in disease. We hypothesized that primary and monocyte-derived macrophages (MDM) represent a suitable vitro model for the identification strain-specific virulence properties at cellular level. Twelve different C. strains were selected to host species multiple loci variable number tandem repeat analysis (MLVA) genotypes. Infection...
Abstract In 2011, a severe outbreak of hemolytic-uremic syndrome was caused by an unusual, highly virulent enterohemorrhagic E. coli (EHEC) O104:H4 strain, which possessed EHEC virulence traits in the genetic background human-adapted enteroaggregative . To determine magnitude fecal shedding and site colonization livestock host, 30 (ten/strain) weaned calves were inoculated with 10 CFU O104:H4, O157:H7 (positive control) or strain 123 (negative necropsied (4 28 d.p.i.). recovered until d.p.i....