A5069258877- Photosynthetic Processes and Mechanisms
- Protist diversity and phylogeny
- Lipid Membrane Structure and Behavior
- Algal biology and biofuel production
- Caveolin-1 and cellular processes
- Bacterial Genetics and Biotechnology
- Nanoparticles: synthesis and applications
- Microbial Community Ecology and Physiology
- Microbial bioremediation and biosurfactants
- Erythrocyte Function and Pathophysiology
- Microbial metabolism and enzyme function
- Blood Coagulation and Thrombosis Mechanisms
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Protease and Inhibitor Mechanisms
- Surfactants and Colloidal Systems
- Complement system in diseases
- Cellular transport and secretion
- Antifungal resistance and susceptibility
- Neuroscience and Neuropharmacology Research
- Cell Adhesion Molecules Research
- Bacterial biofilms and quorum sensing
- Extracellular vesicles in disease
- Biocrusts and Microbial Ecology
- Bacteriophages and microbial interactions
- Streptococcal Infections and Treatments
Jena University Hospital
2016-2025
Friedrich Schiller University Jena
2014-2023
Institute of Human Genetics
2014
Schiller International University
2006-2007
Philipps University of Marburg
1993-1995
The formation of extracellular amyloid plaques is a common patho-biochemical event underlying several debilitating human conditions, including Alzheimer’s disease (AD). Considerable evidence implies that AD damage arises primarily from small oligomeric forms Aβ peptide, but the precise mechanism pathogenicity remains to be established. Using cell culture system reproducibly leads plaques, we show here single plaque represents template-dependent process critically involves presence...
Bacteria frequently exchange metabolites by diffusion through the extracellular environment, yet it remains generally unclear whether bacteria can also use cell–cell connections to directly nutrients. Here we address this question engineering cross-feeding interactions within and between Acinetobacter baylyi Escherichia coli, in which two distant bacterial species reciprocally essential amino acids. We establish that a well-mixed environment E. but likely not A. baylyi, connect other cells...
Background The gut of most insects harbours nonpathogenic microorganisms. Recent work suggests that microbiota not only provide nutrients, but also involve in the development and maintenance host immune system. However, complexity, dynamics types interactions between insect hosts their are far from being well understood. Methods/Principal Findings To determine composition two lepidopteran pests, Spodoptera littoralis Helicoverpa armigera, we applied cultivation-independent techniques based...
Experimental studies in a rat model of fecal peritonitis conducted by Michael Bauer and colleagues show that this model, changes liver function occur early the development sepsis, with potential implications for prognosis new therapeutic approaches.
Summary Filamentous fungi represent classical examples for environmentally acquired human pathogens whose major virulence mechanisms are likely to have emerged long before the appearance of innate immune systems. In natural habitats, amoeba predation could impose a selection pressure towards acquisition attributes. To test this hypothesis, we exploited Dictyostelium discoideum study its interaction with A spergillus fumigatus , two abundant soil inhabitants which found co‐occurrence in...
Summary Most bacterial proteins destined to leave the cytoplasm are exported extracellular compartments or imported into cytoplasmic membrane via highly conserved SecA‐YEG pathway. In present studies, subcellular distributions of core components this pathway, SecA and SecY, secretory protein pre‐AmyQ, were analysed using green fluorescent fusions, immunostaining and/or immunogold labelling techniques. It is shown that SecA, SecY (pre‐)AmyQ located at specific sites near in Bacillus subtilis....
Low density Triton X-100-insoluble plasma membrane microdomains can be isolated from different mammalian cell types and are proposed to involved in trafficking, morphogenesis signal transduction. Heterotrimeric G-proteins their receptors often associated with such domains, suggesting that these structures G-protein-coupled signaling. Here we report detergent-insoluble also exist higher plants contain about 15% of membrane-bound heterotrimeric G-protein beta-subunit (Gbeta). Plasma were...
Axonopathies are a group of clinically diverse disorders characterized by the progressive degeneration axons specific neurons. In hereditary spastic paraplegia (HSP), cortical motor neurons degenerate and cause movement disorder. HSP is linked to mutations in several loci known collectively as genes (SPGs). We identified heterozygous receptor accessory protein 1 (REEP1) exon 2 deletion patient suffering from autosomal dominantly inherited variant SPG31. generated corresponding mouse model...
Upon systemic infection with human pathogenic yeast Candida albicans (C. albicans), monocytes and polymorph nuclear neutrophilic granulocytes are the first immune cells to respond come into contact C. albicans. Monocytes exert immediate candidacidal activity inhibit germination, mediate phagocytosis, kill fungal cells. Here, we show that spontaneously via decondensation of DNA, release this decondensed DNA in form extracellular traps (called monocytic traps: MoETs). Both subtypes...
ABSTRACT The intestine is the primary reservoir of Candida albicans that can cause systemic infections in immunocompromised patients. In this reservoir, fungus exists as a harmless commensal. However, antibiotic treatment disturb bacterial microbiota, facilitating fungal overgrowth and favoring pathogenicity. current vitro gut models are used to study pathogenesis C. investigate state which behaves pathogen rather than We present novel model pathogenicity reduced minimum by increasing...
Abstract Hydrogen-producing bacteria are of environmental importance, since hydrogen is a major electron donor for prokaryotes in anoxic ecosystems. Epsilonproteobacteria currently considered to be hydrogen-oxidizing exclusively. Here, we report production upon pyruvate fermentation free-living Epsilonproteobacteria, Sulfurospirillum spp. The amount produced different two subgroups spp., represented by S. cavolei and multivorans . former produces more excretes acetate as sole organic acid,...
Establishment of multicellularity represents a major transition in eukaryote evolution. A subgroup Amoebozoa, the dictyosteliids, has evolved relatively simple aggregative multicellular stage resulting fruiting body supported by stalk. Protosteloid amoeba, which are scattered throughout amoebozoan tree, differ producing only one or few single stalked spores. Thus, obvious difference developmental cycle protosteliids and dictyosteliids seems to be establishment multicellularity. To separate...
Background: Reduced exercise capacity in patients with heart failure (HF) could be partially explained by skeletal muscle dysfunction. We compared function, structure, and metabolism among clinically stable outpatients HF preserved ejection fraction, reduced healthy controls (HC). Furthermore, the molecular, metabolic, clinical profile of endurance was described. Methods: Fifty-five participants were recruited prospectively at University Hospital Jena (17 18 20 HC). All underwent...
The processes taking place during routine chromosome preparation are not well understood. In this study, the morphological changes in amniotic fluid cells, blood lymphocytes, and bone marrow cells metaphase stage were examined under an inverted microscope preparation. putative that occur simulated suspension, treated with different mixtures of hypotonic solution, fixative, methanol, acetic acid, water. Evaporation fixative was performed normal atmospheric conditions vacuum at levels...
On the one hand, neuronal activity can cause changes in pH; on other pH modulate activity. Consequently, of brain is regulated at various levels. Here we show that steady-state and acid extrusion were diminished cultured hippocampal neurons mice with a targeted disruption Na + -driven Cl − /HCO 3 exchanger Slc4a8. Because Slc4a8 was found to predominantly localize presynaptic nerve endings, hypothesize key regulator pH. Supporting this hypothesis, spontaneous glutamate release CA1 pyramidal...
Abstract Biofilms are social entities where bacteria live in tightly packed agglomerations, surrounded by self-secreted exopolymers. Since production of exopolymers is costly and potentially exploitable non-producers, mechanisms that prevent invasion non-producing mutants hypothesized. Here we study long-term dynamics evolution Bacillus subtilis biofilm populations consisting wild-type (WT) matrix producers mutant non-producers. We show non-producers initially fail to incorporate into...
Several human diseases are associated with a lack of caveolae. Yet, the functions caveolae and molecular mechanisms critical for shaping them still debated. We show that muscle cells syndapin III KO mice severe reductions reminiscent caveolinopathies. different from other mouse models, levels plasma membrane-associated caveolar coat proteins caveolin3 cavin1 were both not reduced upon KO. This allowed dissecting bona fide those supported by mere caveolin presence also demonstrated neither...
The opportunistic fungal pathogen Aspergillus fumigatus can cause life-threatening infections, particularly in immunocompromised patients. Most pathogenic microbes control host innate immune responses at the earliest time, already before infiltrating cells arrive site of infection. Here, we identify Aspf2 as first A. Factor H binding protein. recruits several human plasma regulators, H, FHL-1, FHR1, and plasminogen. contacts via two regions located SCRs6–7 SCR20. FHL-1 binds SCRs6–7, FHR1...
Abstract Extracellular vesicles have an important function in cellular communication. Here, we show that human and mouse monocytes release TGF-β1-transporting response to the pathogenic fungus Candida albicans . Soluble β-glucan from C. binds complement receptor 3 (CR3, also known as CD11b/CD18) on induces of vesicles. CR3-dependence is demonstrated using CR3-deficient (CD11b knockout) generated by CRISPR-CAS9 genome editing isolated mice. These reduce pro-inflammatory M1-macrophages well...
Microbial communities involving dehalogenating bacteria assist in bioremediation of areas contaminated with halocarbons. To understand molecular interactions between bacteria, we co-cultured Sulfurospirillum multivorans, dechlorinating tetrachloroethene (PCE) to cis-1,2-dichloroethene (cDCE), and Dehalococcoides mccartyi strains BTF08 or 195, PCE ethene. The co-cultures were cultivated lactate as electron donor. In co-cultures, the bacterial cells formed aggregates D. established an unusual,...
Abstract Proteinase‐activated receptor‐1 (PAR 1 ), a thrombin receptor and the prototype of newly discovered G‐protein‐coupled subfamily, plays an important role in tumor development progression. In this study, we documented expression receptors PAR , 3 4 permanent hepatocellular carcinoma (HCC) cell lines primary HCC cultures. Stimulation cells with ‐selective activating peptide, TFLLRN‐NH 2 increased transmembrane migration across collagen barrier. This effect was blocked by antagonist SCH...
Insights into mechanisms coordinating membrane remodeling, local actin nucleation, and postsynaptic scaffolding during postsynapse formation are important for understanding vertebrate brain function. Gene knockout RNAi in individual neurons reveal that the F-BAR protein syndapin I is a crucial coordinator of excitatory synapses. Syndapin deficiency caused significant reductions synapse dendritic spine densities. These functions reflected direct, SH3 domain-mediated associations functional...