A5081439485- Bacteriophages and microbial interactions
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Microbial Community Ecology and Physiology
- Microbial infections and disease research
- Evolution and Genetic Dynamics
- Evolutionary Game Theory and Cooperation
- Probiotics and Fermented Foods
- Cell Image Analysis Techniques
- Bacillus and Francisella bacterial research
- Plant-Microbe Interactions and Immunity
- Plant and Biological Electrophysiology Studies
- Plant Parasitism and Resistance
- Mycorrhizal Fungi and Plant Interactions
- Animal Disease Management and Epidemiology
- Vibrio bacteria research studies
- Pickering emulsions and particle stabilization
- Sex work and related issues
- Microbial Metabolism and Applications
- Photoacoustic and Ultrasonic Imaging
- CRISPR and Genetic Engineering
- Oral microbiology and periodontitis research
- Alzheimer's disease research and treatments
University of Ljubljana
2021-2025
Technical University of Denmark
2017-2021
Friedrich Schiller University Jena
2016-2018
Czech Academy of Sciences, Institute of Microbiology
2016
Abstract Biofilms are microbial communities that represent a highly abundant form of life on Earth. Inside biofilms, phenotypic and genotypic variations occur in three-dimensional space time; microscopy quantitative image analysis therefore crucial for elucidating their functions. Here, we present BiofilmQ—a comprehensive cytometry software tool the automated high-throughput quantification, visualization numerous biofilm-internal whole-biofilm properties time.
Abstract Surface superhydrophobicity makes bacterial biofilms very difficult to fight, and it is a combination of their matrix composition complex surface roughness which synergistically protects these biomaterials from wetting. Although trying eradicate with aqueous (antibiotic) solutions common practice, this can be futile approach if the have superhydrophobic properties. To date, there are not many options available reduce liquid repellency or prevent material property developing. Here,...
Bacteria produce diverse specialized metabolites that mediate ecological interactions and serve as a rich source of industrially relevant natural products. Biosynthetic pathways for these are encoded by organized groups genes called biosynthetic gene clusters (BGCs). Understanding the function distribution BGCs provides insight into mechanisms through which microorganisms interact compete. Further, understanding is extremely important biocontrol mining new bioactivities. Here, we...
Abstract Biofilms are social entities where bacteria live in tightly packed agglomerations, surrounded by self-secreted exopolymers. Since production of exopolymers is costly and potentially exploitable non-producers, mechanisms that prevent invasion non-producing mutants hypothesized. Here we study long-term dynamics evolution Bacillus subtilis biofilm populations consisting wild-type (WT) matrix producers mutant non-producers. We show non-producers initially fail to incorporate into...
Microbial biofilms are tightly packed, heterogeneous structures that serve as arenas for social interactions. Studies on Gram negative models reveal during evolution in structured environments like biofilms, isogenic populations commonly diversify into phenotypically and genetically distinct variants. These variants can settle alternative biofilm niches develop new types of interactions greatly influence population productivity. Here, we explore the evolutionary diversification pellicle...
The self-produced biofilm provides beneficial protection for the enclosed cells, but costly production of matrix components makes producer cells susceptible to cheating by nonproducing individuals. Despite detrimental effects nonproducers, biofilms can be heterogeneous, with isogenic nonproducers being a natural consequence phenotypic differentiation processes. For instance, in Bacillus subtilis differ two major components, amyloid fiber protein TasA and exopolysaccharides (EPS),...
Summary The Bacillus phage SPβ has been known for about 50 years, but only a few strains are available. We isolated four new wild‐type of the SPbeta species. Phage vB_BsuS‐Goe14 introduces its prophage into spoVK locus, previously not observed to be used by SPβ‐like phages. Sequence data revealed genome replication strategy and packaging mode extracted 55 prophages from public genomes, thereby discovering three more integration loci one additional type integrase. identified resemble species...
Abstract Microbes commonly display great genetic plasticity, which has allowed them to colonize all ecological niches on Earth. Bacillus subtilis is a soil-dwelling organism that can be isolated from wide variety of environments. An interesting characteristic this bacterium its ability form biofilms complex heterogeneity: individual, clonal cells develop diverse phenotypes in response different environmental conditions within the biofilm. Here, we scrutinized impact number and Rap-Phr family...
Abstract Bacterial quorum sensing (QS) is based on signal molecules (SM), which increase in concentration with cell density. At critical SM concentration, a variety of adaptive genes sharply change their expression from basic level to maximum level. In general, this sharp transition, hallmark true QS, requires an dependent positive feedback loop, where enhances its own production. Some communication systems, like the peptide SM-based ComQXPA system Bacillus subtilis , do not have loop and we...
Rothia is an opportunistic pathogen, particularly life-threatening for the immunocompromised. It associated with pneumonia, endocarditis, peritonitis and many other serious infections, including septicemia. Of note, mucilaginousa produces metabolites that support increase overgrowth of Pseudomonas aeruginosa, one ESKAPE bacteria. Endolysins are considered as antibacterial enzymes derived from bacteriophages selectively efficiently kill susceptible bacteria without harming human cells or...
Highlights•Local Bacillus subtilis isolates carry both common and distinct prophage elements•Phylogeny predicts presence better than geographic origin•Prophages show mutual exclusion co-dependence within host genomes•Most predicted prophages are not stress induced but increase sensitivitySummaryProphages constitute a substantial portion of bacterial genomes, yet their effects on hosts remain poorly understood. We examine the abundance, distribution, activity in using computational laboratory...
<title>Abstract</title> Phage-host interactions that occur in host-associated microbiomes are influenced by a plethora of environmental factors. Mucins glycoproteins representing the largest proportion mucus, found animal digestive tract and on surface certain organs, serving as first line defense against toxins pathogens. Previous studies have shown lytic phages an important influence microbial composition mucosal areas. Our study expands this knowledge to between previously untested...
Abstract Phage-host interactions that occur in host-associated microbiomes are influenced by a plethora of environmental factors. Mucins glycoproteins represent the main component mucus, which is found animal digestive tract and on surface certain organs, serving as first line defense against toxins pathogens. Previous studies have shown lytic phages an important influence microbial composition mucosal areas. Our study expands this knowledge to between previously untested targeting probiotic...
Abstract Biofilms are now considered to be the most abundant form of microbial life on Earth, playing critical roles in biogeochemical cycles, agriculture, and health care. Phenotypic genotypic variations biofilms generally occur three-dimensional space time, therefore often investigated using microscopy. However, quantitative analysis microscopy images presents a key obstacle phenotyping biofilm communities single-cell heterogeneity inside biofilms. Here, we present BiofilmQ, comprehensive...
Genetic competence for the uptake and integration of extracellular DNA is a key process in horizontal gene transfer (HGT), one most powerful forces driving evolution bacteria. In several species, development genetic coupled with cell lysis. Using Bacillus subtilis as model bacterium, we studied role surfactin, biosurfactant antimicrobial lipopeptide, transformation. We showed that surfactin itself promotes lysis release, thereby promoting HGT. These results, therefore, provide evidence...