A5029136297- Nanoparticle-Based Drug Delivery
- Bacteriophages and microbial interactions
- Nanoplatforms for cancer theranostics
- Gold and Silver Nanoparticles Synthesis and Applications
- Radiopharmaceutical Chemistry and Applications
- RNA Interference and Gene Delivery
- Electron and X-Ray Spectroscopy Techniques
- Electrospun Nanofibers in Biomedical Applications
- Photoacoustic and Ultrasonic Imaging
- Immunotherapy and Immune Responses
- Genomics and Phylogenetic Studies
- Barrier Structure and Function Studies
- Radiation Therapy and Dosimetry
- Advanced Electron Microscopy Techniques and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Proteins in Food Systems
- Immune Cell Function and Interaction
- Bacterial Genetics and Biotechnology
- 3D Printing in Biomedical Research
- Hemoglobin structure and function
- Algal biology and biofuel production
- Microbial Community Ecology and Physiology
- Probiotics and Fermented Foods
- Advanced Drug Delivery Systems
- Wound Healing and Treatments
Technical University of Denmark
2016-2025
Ørsted (Denmark)
2021-2024
Lundbeck Foundation
2021
Theranostics (New Zealand)
2020
Stanford University
2009-2015
Materials Science & Engineering
2013
Improved imaging modalities are critically needed for optimizing stem cell therapy. Techniques with real-time content to guide and quantitate implantation especially important in applications such as musculoskeletal regenerative medicine. Here, we report the use of silica-coated gold nanorods a contrast agent photoacoustic quantitation mesenchymal cells rodent muscle tissue. The silica coating increased uptake into more than 5-fold, yet no toxicity or proliferation changes were observed...
Abstract Drug delivery to the brain is hampered by presence of blood-brain barrier, which excludes most molecules from freely diffusing into brain, and tightly regulates active transport mechanisms that ensure sufficient nutrients parenchyma. Harnessing possibility delivering neuroactive drugs way receptors already present on endothelium has been interest for many years. The transferrin receptor special since its expression limited as opposed peripheral endothelium. Here, we investigate...
Increasing cell survival in stem therapy is an important challenge for the field of regenerative medicine.Here, we report theranostic mesoporous silica nanoparticles that can increase through both diagnostic and therapeutic approaches.First, nanoparticle offers ultrasound MRI signal to guide implantation into peri-infarct zone away from most necrotic tissue.Second, serves as a slow release reservoir insulin-like growth factor (IGF)-a protein shown survival.Mesenchymal cells labeled with...
Delivery is one of the most critical obstacles confronting nanoparticle use in cancer diagnosis and therapy. For oncological applications, nanoparticles must extravasate order to reach tumor cells perform their designated task. However, little understanding exists regarding effect shape on extravasation. Herein we real-time intravital microscopic imaging meticulously examine how two different behave across three murine models. The study quantitatively demonstrates that high-aspect ratio...
Gold-core nanoparticles designed for imaging by Raman spectroscopy in patients are generally nontoxic mice, causing only temporary liver inflammation when given intravenously.
Rationale: The ability to treat invalidating neurological diseases is impeded by the presence of blood-brain barrier (BBB), which inhibits transport most blood-borne substances into brain parenchyma. Targeting transferrin receptor (TfR) on surface capillaries has been a popular strategy give preferential accumulation drugs or nanomedicines, but several aspects this targeting remain elusive. Here we report that TfR-targeted gold nanoparticles (AuNPs) can accumulate in and further across BBB...
Soft and electrically active materials are currently being utilized for intelligent systems, including electronic skin, cybernetics, soft robotics, wearable devices. However, fabricating that fulfill the complex requirements of such advanced applications remains a challenge. These attributes include electronic, adhesive, self-healing, flexible, moldable, printable, strong mechanical properties. Inspired by recent interest in transforming monofunctional into multifunctional ones through...
Raman imaging offers unsurpassed sensitivity and multiplexing capabilities. However, its limited depth of light penetration makes direct clinical translation challenging. Therefore, a more suitable way to harness attributes in setting would be couple spectroscopy with endoscopy. The use an accessory endoscope conjunction topically administered tumor-targeting nanoparticles during routine colonoscopy could offer new sensitively detect dysplastic lesions while circumventing Raman's avoiding...
Cell organelles are subcellular structures entrapping a set of enzymes to achieve specific functionality. The incorporation artificial into cells is novel medical paradigm which might contribute the treatment various cell disorders by replacing malfunctioning organelles. In particular, expected be powerful solution in context enzyme replacement therapy since enzymatic malfunction primary cause organelle dysfunction. Although several attempts have been made encapsulate within carrier vehicle,...
Abstract Personalized cancer medicine requires measurement of therapeutic efficacy as early possible, which is optimally achieved by three-dimensional imaging given the heterogeneity cancer. Magnetic resonance (MRI) can obtain images both anatomy and cellular responses, if acquired with a molecular contrast agent. The poor sensitivity MRI has limited development activatable MR agents. To overcome this limitation MRI, novel implementation our caspase-3-sensitive nanoaggregation (C-SNAM) agent...
Bacteria produce diverse specialized metabolites that mediate ecological interactions and serve as a rich source of industrially relevant natural products. Biosynthetic pathways for these are encoded by organized groups genes called biosynthetic gene clusters (BGCs). Understanding the function distribution BGCs provides insight into mechanisms through which microorganisms interact compete. Further, understanding is extremely important biocontrol mining new bioactivities. Here, we...
This study investigates the role of halloysite nanotube as a mineral-based thixotropic admixture to 3D printable geopolymer mortar. The first part this paper focuses on fundamental characterization thermal evolution at 30–1000 °C. In second part, we show how calcination and concentration influence fresh hardened properties 3D-printable It was found that regardless treatment, using only 1–2 wt% can significantly increase rheological buildability mortars without compromising their mechanical...
For three-dimensional (3D) bioprinting to fulfill its promise and enable the automated fabrication of complex tissue-mimicking constructs, there is a need for developing bioinks that are not only printable biocompatible but also have integrated cell-instructive properties. Toward this goal, we here present scalable technique generating nanofiber 3D printing inks with unique tissue-guiding capabilities. Our core methodology relies on tailoring size dispersibility cellulose fibrils through...
Abstract Polyethylene glycol (PEG)ylated Raman‐active gold nanoparticles (PEG‐R‐AuNPs) consist of an interchangeable Raman organic molecule layer held onto a nanocore by silica shell. PEG‐R‐AuNPs have been shown preclinically to increase the sensitivity and specificity spectroscopy, with picomolar multiplexing capabilities. Although clinical trials are being designed use functionalized in various applications (e.g., target dysplastic bowel lesions during colonoscopy), effects these on human...
To determine whether intravenous ferumoxytol can be used to effectively label mesenchymal stem cells (MSCs) in vivo and for tracking of cell transplants.This study was approved by the institutional animal care use committee. Sprague-Dawley rats (6-8 weeks old) were injected with 48 hours prior extraction MSCs from bone marrow. Ferumoxytol uptake these evaluated fluorescence, confocal, electron microscopy compared results traditional ex vivo-labeling procedures. The vivo-labeled subsequently...
By taking advantage of the ability 64Cu to bind nonspecifically gold surfaces, we have developed a methodology embed this radionuclide inside nanoparticles (AuNPs). enables in vivo imaging AuNPs by positron emission tomography (PET). multitude uses within health technology and are useful tools for general nanoparticle research. 64Cu-AuNPs were prepared incubating AuNP seeds with 64Cu2+, followed entrapment grafting on second layer gold. This resulted radiolabeling efficiencies 53 ± 6%. The...
Controlled self-assembly of small molecule gadolinium (Gd) complexes into nanoparticles (GdNPs) is emerging as an effective approach to design activatable magnetic resonance imaging (MRI) probes and amplify the r₁ relaxivity. Herein, we employ a reduction-controlled macrocyclization reaction develop redox activated Gd-based MRI probe for sensing reducing environment. Upon disulfide reduction at physiological conditions, acyclic contrast agent 1 containing dual Gd-chelates undergoes...
While transfusion of donor blood is a reasonably safe and well-established procedure, artificial oxygen carriers offer several advantages over transfusions. These benefits include compatibility with all types, thus avoiding the need for cross matching, availability, lack infection, long-term storage. Hemoglobin (Hb)-based (HBOCs) are being explored as an "oxygen bridge" to replace or complement standard transfusions in extreme, life-threatening situations such trauma remote locations austere...
Despite being an indispensable clinical procedure, the transfusion of donor blood has important limitations including a short shelf-life, limited availability and specific storage requirements. Therefore, lot effort been devoted to developing hemoglobin (Hb)-based oxygen carriers (HBOCs) that are able replace or complement standard transfusions, especially in extreme life-threatening situations. Herein, we employed Hb-loaded poly(lactide- co -glycolide) core which was subsequently coated...
In the marine environment, surface-associated bacteria often produce an array of antimicrobial secondary metabolites, which have predominantly been perceived as competition molecules. However, they may also affect other hallmarks living, such motility and biofilm formation. Here, we investigate ecological significance antibiotic metabolite, tropodithietic acid (TDA), in producing bacterium, Phaeobacter piscinae S26. We constructed a markerless in-frame deletion mutant deficient TDA...