A5089610336- Nanoplatforms for cancer theranostics
- Gold and Silver Nanoparticles Synthesis and Applications
- Spectroscopy Techniques in Biomedical and Chemical Research
- Photoacoustic and Ultrasonic Imaging
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Biosensors and Analytical Detection
- Cell Adhesion Molecules Research
- Advanced MRI Techniques and Applications
- Immunotherapy and Immune Responses
- Medical Imaging Techniques and Applications
- Photodynamic Therapy Research Studies
- RNA Interference and Gene Delivery
- Radiopharmaceutical Chemistry and Applications
- Extracellular vesicles in disease
- CAR-T cell therapy research
- Advanced Biosensing Techniques and Applications
- Atomic and Subatomic Physics Research
- Advanced Fluorescence Microscopy Techniques
- T-cell and B-cell Immunology
- Optical Imaging and Spectroscopy Techniques
- Nanocluster Synthesis and Applications
- Appendicitis Diagnosis and Management
- bioluminescence and chemiluminescence research
- Monoclonal and Polyclonal Antibodies Research
Memorial Sloan Kettering Cancer Center
2014-2024
Dana-Farber Cancer Institute
2019-2023
Harvard University
2003-2023
Brigham and Women's Hospital
2008-2023
Cornell University
2014-2022
Kettering University
2018-2022
Stanford University
2012-2014
Hospital for Special Surgery
2012
LI-COR Biosciences (United States)
2012
Beth Israel Deaconess Medical Center
2006-2009
Monocytes participate importantly in the pathogenesis of atherosclerosis, but their spatial and temporal recruitment from circulation remains uncertain. This study tests hypothesis that monocyte accumulation atheroma correlates with extent disease by using a sensitive simple quantitative assay allows tracking highly enriched populations blood monocytes. A two-step isolation method yielded viable functionally intact peripheral (>90%). Recipient mice received syngeneic monocytes labeled two...
Surface-enhanced resonance Raman scattering gold nanostars allow detection of macro- and microscopic foci premalignant cancerous lesions in vivo.
The ability to control the movement of nanoparticles remotely and with high precision would have far-reaching implications in many areas nanotechnology. We designed a unique dynamic magnetic field (DMF) generator that can induce rotational movements superparamagnetic iron oxide (SPIONs). examined whether nanoparticle could be used for remote induction cell death by injuring lysosomal membrane structures. further hypothesized shear forces created generation oscillatory torques (incomplete...
The current difficulty in visualizing the true extent of malignant brain tumors during surgical resection represents one major reasons for poor prognosis tumor patients. Here, we evaluated ability a hand-held Raman scanner, guided by surface-enhanced scattering (SERS) nanoparticles, to identify microscopic genetically engineered RCAS/tv-a glioblastoma mouse model. In simulated intraoperative scenario, tested both static imaging device and mobile, scanner. We show that SERS image-guided is...
A number of quantitative three-dimensional tomographic near-infrared fluorescence imaging techniques have recently been developed and combined with MR to yield highly detailed anatomic molecular information in living organisms (1, 2). Here we describe magnetic nanoparticle based contrast agents that a (NIRF) is activated by certain enzymes. The probes are prepared conjugation arginyl peptides cross-linked iron oxide amine (amino-CLIO), either disulfide linkage or thioether linker, followed...
Surface modification of superparamagnetic contrast agents with HIV-1 tat peptide has emerged as a promising means for intracellular magnetic labeling and noninvasive tracking large number cell types MRI. To achieve efficient delivery the nanoparticles, we investigated effect on cellular uptake iron oxide particles by varying attached peptides. First, report here modified P2T method in measuring numbers surface attachments per particle through disulfide linkage. The was shown to have...
Complete surgical resection is the ideal first-line treatment for most liver malignancies. This goal would be facilitated by an intraoperative imaging method that enables more precise visualization of tumor margins and detection otherwise invisible microscopic lesions. To this end, we synthesized silica-encapsulated surface-enhanced Raman scattering (SERS) nanoparticles (NPs) act as a molecular agent We hypothesized that, after intravenous administration, SERS NPs avidly home to healthy...
Ovarian cancer has a unique pattern of metastatic spread, in that it initially spreads locally within the peritoneal cavity. This is contrast to most other types, which metastasize early on via bloodstream distant sites. behavior opens up an opportunity for local application both therapeutic and imaging agents. Upon initial diagnosis, 75% patients already present with diffuse spread involving abdominal organs. Complete resection all tumor implants been shown be major factor improved...
High sensitivity and specificity are two desirable features in biomedical imaging. Raman imaging has surfaced as a promising optical modality that offers both. Here we report the design synthesis of group near-infrared absorbing 2-thienyl-substituted chalcogenopyrylium dyes tailored to have high affinity for gold. When adsorbed onto gold nanoparticles, these produce biocompatible SERRS nanoprobes with attomolar limits detection amenable ultrasensitive vivo multiplexed tumour disease marker detection.
Research activity in medical and cancer nanotechnology has grown dramatically over the past 15 years. The field become a cradle of multidisciplinary investigations bringing together physicists, chemists, engineers working with clinicians biologists to address paramount problems care treatment. Some have argued that explosion number research papers not been followed by sufficient clinical nanomedicine. However, three new nanodrugs now approved U.S. Food Drug Administration (FDA) years,...
Rationale:The goal of imaging tumors at depth with high sensitivity and specificity represents a significant challenge in the field biomedical optical imaging.'Surface enhanced Raman scattering' (SERS) nanoparticles (NPs) have been employed as image contrast agents can be used to specifically target cells vivo.By tracking their unique "fingerprint" spectra, it becomes possible determine precise location.However, while detection SERS NPs is very sensitive specific, conventional spectroscopy...
Chelator-free nanoparticles for intrinsic radiolabeling are highly desirable whole-body imaging and therapeutic applications. Several reports have successfully demonstrated the principle of radiolabeling. However, work done to date has suffered from much same specificity issues as conventional molecular chelators, insofar there is no singular nanoparticle substrate that proven effective in binding a wide library radiosotopes. Here we present amorphous silica general substrates chelator-free...
A handheld approach to optoacoustic imaging is essential for the clinical translation. The first 2- and 3-dimensional multispectral tomography (MSOT) probes featuring real-time unmixing have recently been developed. Imaging performance of both was determined in vitro a brain melanoma metastasis mouse model vivo. T1-weighted MR images were acquired anatomical reference. limit detection cells significantly lower using 2D than 3D probe. signal decrease more profound relation depth with versus...
Abstract Recently, surface-enhanced Raman scattering nanoprobes have shown tremendous potential in oncological imaging owing to the high sensitivity and specificity of their fingerprint-like spectra. As current scanners rely on a slow, point-by-point spectrum acquisition, there is an unmet need for faster cover clinically relevant area real-time. Herein, we report rational design optimization fluorescence-Raman bimodal nanoparticles (FRNPs) that synergistically combine spectroscopy with...
The dismal prognosis of patients with malignant brain tumors such as glioblastoma multiforme (GBM) is attributed mostly to their diffuse growth pattern and early microscopic tumor spread distant regions the brain.Because foci cannot be visualized current imaging modalities, it remains impossible direct treatments optimally.Here we explored ability integrin-targeted surface-enhanced resonance Raman spectroscopy (SERRS) nanoparticles depict true extent in a GBM mouse model that closely mimics...
Recently, surface‐enhanced Raman scattering (SERS) nanoprobes (NPs) have shown promise in the field of cancer imaging due to their unparalleled signal specificity and high sensitivity. This study reports development a DNA aptamer targeted SERS NP. aptamers are being investigated as viable alternative more traditional antibody targeting low immunogenicity cost production. A strategy is developed functionalize NPs with aptamers, which target Mucin1 (MUC1) human breast (BC). Thorough vitro...